1990
DOI: 10.1271/bbb1961.54.2429
|View full text |Cite
|
Sign up to set email alerts
|

Isolation and characterization of sucrose:sucrose 1F-.BETA.-D-fructosyltransferase from tubers of Helianthus tuberosus L.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

3
7
1

Year Published

1993
1993
2014
2014

Publication Types

Select...
4
3

Relationship

0
7

Authors

Journals

citations
Cited by 13 publications
(11 citation statements)
references
References 1 publication
3
7
1
Order By: Relevance
“…Gel filtration predicted a molecular mass of 69 kD for this protein, which corresponds well with 1-SST from fructan-accumulating tubers (gel filtration data in this report). However, the temperature optimum (34"C), the pH optimum (5.4), and the activity profile as a function of the [SUC] ( K , of 42 mM) of the enzyme from dormant tubers (Praznik et al, 1990) differ from those of the enzyme described in the present paper. The enzyme purified from dormant tubers may therefore represent an SST isoform that is not active or present in fructan-accumulating tubers.…”
Section: Discussioncontrasting
confidence: 70%
See 2 more Smart Citations
“…Gel filtration predicted a molecular mass of 69 kD for this protein, which corresponds well with 1-SST from fructan-accumulating tubers (gel filtration data in this report). However, the temperature optimum (34"C), the pH optimum (5.4), and the activity profile as a function of the [SUC] ( K , of 42 mM) of the enzyme from dormant tubers (Praznik et al, 1990) differ from those of the enzyme described in the present paper. The enzyme purified from dormant tubers may therefore represent an SST isoform that is not active or present in fructan-accumulating tubers.…”
Section: Discussioncontrasting
confidence: 70%
“…The most recent study on the purification of 1-SST from Asteraceae describes a procedure to obtain the enzyme from dormant tubers of H. tuberosus in two chromatographic steps (Praznik et al, 1990). Gel filtration predicted a molecular mass of 69 kD for this protein, which corresponds well with 1-SST from fructan-accumulating tubers (gel filtration data in this report).…”
Section: Discussionsupporting
confidence: 59%
See 1 more Smart Citation
“…A further study on the purification of SST from roots oi Helianthus tuberosus (Praznik, Beck & Spies, 1990) provided no evidence for in vitro synthesis of trisaccharide or larger fructan. It is concluded that, with the exception of trisaccharide, there is no evidence for the de novo enzymatic synthesis of inulin from sucrose by enzymes from roots of Helianthus tuberosus.…”
Section: Internal Contradiction Within the Modelmentioning
confidence: 91%
“…This step is catalyzed by sucrose:sucrose fructosyl transferase (1-SST; EC 2.4.1.99), a vacuolar enzyme isolated and purified from a wide variety of tissues (Pollock and Suzuki, 1986;Praznik et al, 1990;Angenent et al, 1993;Koops and Jonker, 1996;. Although demonstrated both in vivo (Cairns et al, 1989;Hellwege et al, 1997) and in vitro (Bancal and Gaudillere, 1993;Cairns, 1993;Penson and Cairns, 1994;Lu¨scher et al, 1996;Itaya et al, 1997;Vijn et al, 1998), synthesis of 1-kestose by 1-SST has remained ambiguous in light of the enzyme's unusually high K m for sucrose which varies from 75 mM up to over 500 mM depending on the tissue investigated and assay conditions (Chatterton et al, 1988;Cairns and Ashton, 1994).…”
Section: Introductionmentioning
confidence: 99%