Isolation and Characterization of Subviral Structures from Tomato Spotted Wilt Virus

Mohamed, N. A.

doi:10.1099/0022-1317-53-2-197

Cited by 40 publications

(25 citation statements)

References 6 publications

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“…The virus contains three ssRNA segments, S (2.9 kb), M (5.0 kb) and L (8.9 kb). Each RNA segment is associated with nucleocapsid (N) proteins to form pseudo-circular nucleocapsid structures (van den Hurk et al, 1977;Mohamed, 1981;de Haan et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments

Kormelink¹,

Haan²,

Meurs³

et al. 1992

Journal of General Virology

200

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The complete sequence of the tomato spotted wilt virus (TSWV) M RNA segment has been determined. The RNA is 4821 nucleotides long and has an ambisense coding strategy similar to that of the S RNA segment. The M RNA segment contains two open reading frames (ORFs), one in the viral sense which encodes a protein with a predicted size of 33-6K, and one in the viral complementary sense which encodes the precursor to the G 1 and G2 glycoproteins, with a predicted size of 127.4K. Both ORFs are expressed via the synthesis of subgenomic mRNAs that possibly terminate at a stable hairpin structure, located in the intergenic region. The precursor for the glycoproteins contains a sequence motif (RGD) which is characteristic of cellular attachment domains. Significant sequence homology was found between the G1 glycoproteins of members of the genus Bunyavirus and a corresponding region in the glycoprotein precursor of TSWV, indicating a close evolutionary relationship between these viruses. With the elucidation of the M RNA sequence, the complete nucleotide sequence of TSWV has been determined. TSWV represents the first member of the Bunyaviridae shown to contain two ambisense RNA segments.

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Section: Introductionmentioning

confidence: 99%

The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments

Kormelink¹,

Haan²,

Meurs³

et al. 1992

Journal of General Virology

200

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“…The genome consists of three linear ssRNA molecules approximately 3100 (S RNA), 5400 (M RNA) and 8200 (L RNA) nucleotides long, each complexed with N proteins to form circular nucleocapsids (van den Hurk et al, 1977;Mohamed, 1981;de Haan et al, 1989a). Based on this and other properties it has been suggested that TSWV should be considered as a member of the arthropod-borne Bunyaviridae (Milne & Francki, 1984;de Haan et al, 1989 a).…”

Section: Introductionmentioning

confidence: 99%

The S RNA Segment of Tomato Spotted Wilt Virus has an Ambisense Character

Haan

Wagemakers

Peters

et al. 1990

Journal of General Virology

277

130

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The complete nucleotide sequence of the S RNA of tomato spotted wilt virus (TSWV) was determined. The RNA is 2916 nucleotides long and has an ambisense coding strategy. The sequence contains two open reading frames (ORFs), one in the viral sense which encodes a protein with a predicted Mr of 52"4K and one in the viral complementary sense which encodes the viral nucleocapsid protein of Mr 28"8K. Both proteins are expressed by translation of two subgenomic RNA species that possibly terminate at a long stable hairpin structure, located at the intergenic region. The structure of this RNA segment resembles that of the arthropod-borne phleboviruses (family Bunyaviridae). The absence of significant sequence homology between TSWV and bunyaviruses infecting animals suggests that TSWV should be considered as a representative of a new genus within the Bunyaviridae.

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“…The internal pseudo-circular nucleocapsids consist of three species of ssRNA, denoted S RNA (2916 nucleotides), M RNA (approximately 5000 nucleotides) or L RNA (approximately 8000 nucleotides), which are tightly encapsidated with the nucleocapsid (N) protein (Mr 28.8K) (de Haan et al, 1989 b). In addition a few copies of a large (L) protein (approximately 200K) are present in the virus particle, and may represent the viral polymerase (Mohamed et al, 1973;Mohamed, 1981;Tas et al, 1977).…”

Section: Introductionmentioning

confidence: 99%