Abstract:In heat stress conditions, E. coli will produce EF-Tu protein abundantly under the regulation of str promoter. The str promoter from E. coli is known to have sequences that are recognized by two sigma factors (σ70 and σS). The aim of this research was to isolate and characterize str promoter from E. coli DH5a using reporter gene AmilCP. The str promoter from E. coli DH5α (289 bp) had been isolated by Colony PCR using specific primers. Bioinformatics analysis of str promoter showed high conserved areas (100%) f… Show more
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