ISOLATION AND CHARACTERIZATION OF STIGMASTEROL AND -SITOSTEROL FROM WOOD BARK EXTRACT OF Baccaurea macrocarpa Miq. Mull. Arg

Erwin, Erwin; Pusparohmana, Widar R.; Safitry, Rizka D.; Marliana, Eva; Usman, Usman; Kusuma, Irawan Wijaya

doi:10.31788/rjc.2020.1345652

Cited by 9 publications

(3 citation statements)

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“…The fractions were also found to be non-toxic. 49 The highest radical DPPH activity of soybean may be due to gamma-tocopherol, 50 which is similar to our GC-MS findings that revealed the presence of gamma-tocopherol in soybean oil. Also, the presence of isoflavones in soybean is associated with significant antioxidant activity.…”

Section: Percentage Radical Scavenging Activity Of Soybean Oilsupporting

confidence: 87%

Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization

Khan¹,

Arshad

Arif

et al. 2022

Dose-Response

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The current study aims to isolate, purify, and characterize the trypsin inhibitor protein from seeds of soya beans, scientifically known as Glycine max. Its seeds were ground, and the powder was soaked several times using n-hexane. It was added to phosphate buffer saline (PBS) followed by filtration and centrifugation of the PBS dissolved extract. The supernatant was subjected to ammonium sulfate precipitation and about six fractions, 30% to 80% were prepared. The centrifuged pellets obtained from each fraction were dialyzed and run on SDS-PAGE. The trypsin inhibitor protein was precipitated and characterized in 30% pellet and molecular weight was 21.5 kDa compared to protein ladder (ThermoFisher 10-170 kDa). GC-MS analysis revealed the steroid derivatives such as stigmasterol, campesterol, beta-sitosterol, and gamma-tocopherol. Glycine max trypsin inhibitor could be used as a plant-derived drug to overcome the over-activation of trypsin without its real substrate (proteins) becoming activated and start auto digestion leading to pancreatitis.

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Section: Percentage Radical Scavenging Activity Of Soybean Oilsupporting

confidence: 87%

Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization

Khan¹,

Arshad

Arif

et al. 2022

Dose-Response

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“…13 Campesterol and β-sitosterol were isolated from the extract after chromatographic separation, and their structures ( Figure 2 , Figure 3 ) were identified with spectroscopic investigations and comparison with data from the literature. 18 , 19 , 20 , 21 , 22 , 23 These compounds are present in a variety of plant species; their biological activities have been thoroughly investigated, and their pharmaceutical effects have been established. The antifungal activity of campesterol and β-sitosterol obtained from Dendrocalamus asper against some fungal pathogens has been determined, and they have been found to have exceptional antifungal properties.…”

Section: Resultsmentioning

confidence: 99%

Isolation, characterization, and docking studies of campesterol and β-sitosterol from Strychnos innocua (Delile) root bark

Uttu¹,

Sallau

Ibrahim

et al. 2023

Journal of Taibah University Medical Sciences

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“…The EtOH extract of the stem bark of T. monadelpha was separated into four fractions with liquid–liquid partition using EtOAc and n-BuOH, respectively. The EtOAc fraction was subjected to repeated silica gel and Sephadex LH-20 column chromatography to afford thirteen compounds, including 24-acetoxy-21,25-dihydroxy-21,23-epoxytirucall-7-en-3-one ( 1 ), (6 R )-1- O -deacetylkhayanolide E ( 2 ), melianodiol ( 3 ) [ 27 ], (+)-21 R *,23 R *-epoxy-21 α -methoxy-24,25-dihydroxyapotirucall-7-en-3-one ( 4 ) [ 28 ], khayanolide D ( 5 ) [ 29 ], grandifotane A ( 6 ) [ 30 ], betulin ( 7 ), betulinic acid ( 8 ) [ 31 ], epicatechin ( 9 ) [ 32 ], stigmastane-3,6-dione ( 10 ) [ 33 ], tetracosanoic acid ( 11 ) [ 34 ], the mixture of β -sitosterol ( 12a ) and stigmasterol ( 12b ) [ 35 ], and β -sitosterol 3-O -β -D-glucopyranoside ( 13 ) [ 36 ] ( Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

Constituents of the Stem Bark of Trichilia monadelpha (Thonn.) J. J. De Wilde (Meliaceae) and Their Antibacterial and Antiplasmodial Activities

et al. 2023

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The chemical investigation of the EtOH extract from the stem bark of Trichilia monadelpha (Thonn.) J. J. De Wilde afforded two new limonoids (1 and 2): 24-acetoxy-21,25-dihydroxy-21,23-epoxytirucall-7-en-3-one (1) and (6R)-1-O-deacetylkhayanolide E (2), together with eleven known compounds (3–13), including additional limonoids, flavonoids, triterpenoids, steroids, and fatty acid. Their structures were determined using 1D- and 2D-NMR experiments, ESI mass spectrometry, and single crystal X-ray diffraction analysis. The antibacterial and antiplasmodial activities of the extracts, sub-extracts, fractions, and some of the isolated compounds were evaluated in known pathogenic strains, including Staphylococcus aureus and Plasmodium falciparum. Fraction E (n-Hex/EtOAc 30:70, v/v) showed significant activity against S. aureus ATCC 25923 with a MIC value of 3.90 µg/mL, while one of its constituents (epicatechin (9)) exhibited significant activity with MIC values of 7.80 µg/mL. Interestingly, grandifotane A (6) (IC50 = 1.37 µM) and khayanolide D (5) (IC50 = 1.68 µM) were highly active against the chloroquine-sensitive/sulfadoxine-resistant plasmodium falciparum 3D7 strain, unlike their corresponding plant extract and fractions.

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ISOLATION AND CHARACTERIZATION OF STIGMASTEROL AND -SITOSTEROL FROM WOOD BARK EXTRACT OF Baccaurea macrocarpa Miq. Mull. Arg

Cited by 9 publications

References 0 publications

Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization

Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization

Isolation, characterization, and docking studies of campesterol and β-sitosterol from Strychnos innocua (Delile) root bark

Constituents of the Stem Bark of Trichilia monadelpha (Thonn.) J. J. De Wilde (Meliaceae) and Their Antibacterial and Antiplasmodial Activities

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