Isolation and characterization of Staphylococcus aureus starvat ion4 nduced, stationary-phase mutants defective in survival or recovery

Watson, Steve P.; Antonio, Martín; Foster, Simon J.

doi:10.1099/00221287-144-11-3159

Cited by 51 publications

(75 citation statements)

References 54 publications

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“…The other mutants lacked SodA, which has been discussed above; RpoE, which seems to play a regulatory role in stationary phase; a protein of unknown function; and UmuC. UmuC plays a role in the SOS response in B. subtilis, i.e., the response induced in response to DNA damage (242), again demonstrating the importance of DNA repair systems in the response to low pH (245).…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

Cotter

Hill

2003

Microbiol Mol Biol Rev

1,032

828

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Gram-positive bacteria possess a myriad of acid resistance systems that can help them to overcome the challenge posed by different acidic environments. In this review the most common mechanisms are described: i.e., the use of proton pumps, the protection or repair of macromolecules, cell membrane changes, production of alkali, induction of pathways by transcriptional regulators, alteration of metabolism, and the role of cell density and cell signaling. We also discuss the reponses of Listeria monocytogenes, Rhodococcus, Mycobacterium, Clostridium perfringens, Staphylococcus aureus, Bacillus cereus, oral streptococci, and lactic acid bacteria to acidic environments and outline ways in which this knowledge has been or may be used to either aid or prevent bacterial survival in low-pH environments

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Section: Staphylococcus Aureusmentioning

confidence: 99%

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

Cotter

Hill

2003

Microbiol Mol Biol Rev

1,032

828

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“…Starvation survival experiments were carried out in glucose-limiting CDM medium, with shaking at 250 rpm at 37°C (55). Desiccation tolerance testing was performed on stationary-phase cells grown in CT-BHI, washed, and resuspended in PBS to an OD 600 of 0.05.…”

Section: Methodsmentioning

confidence: 99%

Catalase (KatA) and Alkyl Hydroperoxide Reductase (AhpC) Have Compensatory Roles in Peroxide Stress Resistance and Are Required for Survival, Persistence, and Nasal Colonization inStaphylococcus aureus

et al. 2007

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Oxidative-stress resistance in Staphylococcus aureus is linked to metal ion homeostasis via several interacting regulators. In particular, PerR controls the expression of a regulon of genes, many of which encode antioxidants. Two PerR regulon members, ahpC (alkylhydroperoxide reductase) and katA (catalase), show compensatory regulation, with independent and linked functions. An ahpC mutation leads to increased H 2 O 2 resistance due to greater katA expression via relief of PerR repression. Moreover, AhpC provides residual catalase activity present in a katA mutant. Mutation of both katA and ahpC leads to a severe growth defect under aerobic conditions in defined media (attributable to lack of catalase activity). This results in the inability to scavenge exogenous or endogenously produced H 2 O 2 , resulting in accumulation of H 2 O 2 in the medium. This leads to DNA damage, the likely cause of the growth defect. Surprisingly, the katA ahpC mutant is not attenuated in two independent models of infection, which implies reduced oxygen availability during infection. In contrast, both AhpC and KatA are required for environmental persistence (desiccation) and nasal colonization. Thus, oxidative-stress resistance is an important factor in the ability of S. aureus to persist in the hospital environment and so contribute to the spread of human disease.

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“…To measure growth in the presence of high-salt concentrations, cultures were inoculated from overnight BHI cultures to an OD 600 value of 0?08 into either BHI or BHI containing 2 M NaCl at 37 uC. Hydrogen peroxide resistance assays were carried out as described previously (Watson et al, 1998a) with the following modifications. Cells were grown in amino-acid-limiting CDM (1 %, w/v, glucose) to exponential phase (OD 600 0?1).…”

Section: Methodsmentioning

confidence: 99%

“…The Tn917 insertion mutant SPW20 was isolated in a previous screen for starvation survival mutants of S. aureus (Watson et al, 1998a). In this strain, the transposon is inserted in an ORF of 185 aa that shares 59 % identity with the putative hypoxanthine-guanine phosphoribosyltransferase (HprT) enzyme from B. subtilis.…”

Section: Identification and Mutagenesis Of Hprt And Ftshmentioning

confidence: 99%

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Role of the hprT–ftsH locus in Staphylococcus aureus

2004

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The roles of two adjacent genes in the Staphylococcus aureus chromosome with functions in starvation survival and the response to stressful conditions have been characterized. One of these, hprT, encoding a hypoxanthine-guanine phosphoribosyltransferase homologue, was initially identified in a transposon mutagenesis screen. Mutation of hprT affects starvation survival in amino-acid-limiting conditions and the ability of S. aureus to grow in high-salt concentrations. Downstream of hprT is ftsH, which encodes a membrane-bound, ATP-and Zn 2+ -dependent 'AAA'-type protease. Mutation of ftsH in S. aureus leads to pleiotropic defects including slower growth, sensitivity to salt, acid, methyl viologen and potassium tellurite stresses, and reduced survival in amino-acid-or phosphate-limiting conditions. Both hprT-lacZ and ftsH-lacZ gene fusions are expressed maximally in the post-exponential phase of growth. Although secretion of exoproteins is not affected, an ftsH mutant is attenuated in a murine skin lesion model of pathogenicity.

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Isolation and characterization of Staphylococcus aureus starvat ion4 nduced, stationary-phase mutants defective in survival or recovery

Cited by 51 publications

References 54 publications

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

Catalase (KatA) and Alkyl Hydroperoxide Reductase (AhpC) Have Compensatory Roles in Peroxide Stress Resistance and Are Required for Survival, Persistence, and Nasal Colonization inStaphylococcus aureus

Role of the hprT–ftsH locus in Staphylococcus aureus

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