Isolation and characterization of spontaneous phage-resistant mutants of Lactobacillus paracasei

Sunthornthummas, Sirinthorn; Doi, Kunio; Rangsiruji, Achariya; Krajangsung, Sukhumaporn; Sarawaneeyaruk, Siriruk; Pringsulaka, Onanong

doi:10.1016/j.foodcont.2018.12.037

Cited by 3 publications

(2 citation statements)

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“…We found an SC method isolation efficiency of 57.6%, while the AP method efficiency was much lower (25.0%). The higher efficiency of the SC method has been reported before though with similar, smaller or larger differences ( Guglielmotti et al, 2006 ; Binetti et al, 2007 ; Sunthornthummas et al, 2019 ). The lower AP efficiency (especially at low MOIs) could be explained by a low selection pressure for phage resistance.…”

Section: Discussionsupporting

confidence: 63%

“…Using a combined approach of the SC and AP method resulted in an overall mutant isolation efficiency of ∼41%. Previous studies using this approach found an isolation efficiency of true resistant Lactobacillus paracasei isolates of 56% ( Sunthornthummas et al, 2019 ) and an average isolation efficiency of 36.5% (ranging between 29.5 and 50%) of true resistant Lactobacillus delbrueckii isolates ( Guglielmotti et al, 2006 ). We found an SC method isolation efficiency of 57.6%, while the AP method efficiency was much lower (25.0%).…”

Section: Discussionmentioning

confidence: 99%

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Spontaneous Phage Resistance in Avian Pathogenic Escherichia coli

et al. 2021

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Avian pathogenic Escherichia coli (APEC) is one of the most important bacterial pathogens affecting poultry worldwide. The emergence of multidrug-resistant pathogens has renewed the interest in the therapeutic use of bacteriophages (phages). However, a major concern for the successful implementation of phage therapy is the emergence of phage-resistant mutants. The understanding of the phage-host interactions, as well as underlying mechanisms of resistance, have shown to be essential for the development of a successful phage therapy. Here, we demonstrate that the strictly lytic Escherichia phage vB_EcoM-P10 rapidly selected for resistance in the APEC ST95 O1 strain AM621. Whole-genome sequence analysis of 109 spontaneous phage-resistant mutant strains revealed 41 mutants with single-nucleotide polymorphisms (SNPs) in their core genome. In 32 of these, a single SNP was detected while two SNPs were identified in a total of nine strains. In total, 34 unique SNPs were detected. In 42 strains, including 18 strains with SNP(s), gene losses spanning 17 different genes were detected. Affected by genetic changes were genes known to be involved in phage resistance (outer membrane protein A, lipopolysaccharide-, O- antigen-, or cell wall-related genes) as well as genes not previously linked to phage resistance, including two hypothetical genes. In several strains, we did not detect any genetic changes. Infecting phages were not able to overcome the phage resistance in host strains. However, interestingly the initial infection was shown to have a great fitness cost for several mutant strains, with up to ∼65% decrease in overall growth. In conclusion, this study provides valuable insights into the phage-host interaction and phage resistance in APEC. Although acquired resistance to phages is frequently observed in pathogenic E. coli, it may be associated with loss of fitness, which could be exploited in phage therapy.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Spontaneous Phage Resistance in Avian Pathogenic Escherichia coli

et al. 2021

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RNA-seq reveals the phage-resistant mechanisms displayed by Lactiplantibacillus plantarum ZJU-1 isolated from Chinese traditional sourdough

Yuan

Fan

Zhao

et al. 2022

International Dairy Journal

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Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02

Gao

Zhang

et al. 2023

Foods

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Limosilactobacillus fermentum is a bacterium widely used in food production, medicine, and industrial fermentation. However, fermentation could fail due to phage contamination. L. fermentum bacteriophage LFP02 can be induced from L. fermentum IMAU 32579 using mitomycin C. To better understand the characteristics of this phage, its physiological and genomic characteristics were evaluated. The results showed that its optimal multiplicity of infection was 0.01, and the burst size was 148.03 ± 2.65 pfu/infective center. Compared to temperature, pH had a more obvious influence on phage viability, although its adsorption capacity was not affected by the divalent cations (Ca2+ and Mg2+) or chloramphenicol. Its genome size was 43,789 bp and the GC content was 46.06%, including 53 functional proteins. Compared to other L. fermentum phages, phage LFP02 had chromosome deletion, insertion, and inversion, which demonstrated that it was a novel phage. This study could expand the knowledge of the biological characteristics of L. fermentum bacteriophages and provide some theoretical basis for bacteriophage prevention during fermentation.

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Isolation and characterization of spontaneous phage-resistant mutants of Lactobacillus paracasei

Cited by 3 publications

References 42 publications

Spontaneous Phage Resistance in Avian Pathogenic Escherichia coli

Spontaneous Phage Resistance in Avian Pathogenic Escherichia coli

RNA-seq reveals the phage-resistant mechanisms displayed by Lactiplantibacillus plantarum ZJU-1 isolated from Chinese traditional sourdough

Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02

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