Isolation and Characterization of Some Phytochemicals from Indian Traditional Plants

Abstract

The present study was designed to evaluate relative contribution of different polyphenols (total phenolics, flavonoids, flavonols) and their antioxidants activities in aqueous extracts of different parts of some plants; Argemone mexicana, Datura metel, Calotropis procera, Thevetia peruviana, and Cannabis sativa. The antioxidants (total phenolics, flavonoids, flavones) were determined by chemical methods. The antioxidant capacities of these extracts were evaluated by FRAP assay. The results demonstrated that phenolic content was maximally present in leaves of T. peruviana. This plant exhibited minimum phenolic content in its flower as compared to other plants. The flower of D. metel contained maximum phenolic content. The flavonoids were present in highest quantity in leaves of C. procera while T. peruviana flowers showed maximum flavonoid content. The fruits of C. sativa contained maximum quantity of flavonoid as compared to other plants tested. The flower extract of C. sativa possessed highest FRAP value followed by A. mexicana and fruit of C. procera. The values of ratios of different polyphenolic compounds present in plant extracts indicated that flower of D. metel contained maximum total flavonoids and minimum phenolics. These results suggested that levels of total phenolics, flavonoids and their FRAP indices exhibited specificity to different plants and their parts.

Antifungal activity of Datura metel L. organic and aqueous extracts on some pathogenic and antagonistic fungi

“…Saponin has been reported to have antifungal capacities (Sparg et al, 2004). Also, the amount of phenolic and flavonoids content on aqueous extracts of D. metel aerial parts was determined by Srivastava et al (2012). The results showed that the maximum phenolic content was present in flower (19.75 mg/g) and the highest flavonoids content was found in leaves (2 mg/g).…”

Pure Cannabidiol versus Cannabidiol-Containing Extracts: Distinctly Different Multi-Target Modulators

“…In plants, they are essential pigments and are found in flowers, leaves and stems giving them the typical color but add also to the smell and flavor of a particular Cannabis strain. Flowers of hemp rank among the plants with the highest flavonoid content and antioxidant power; the total content in the Cannabis leaves and flowers can reach 2.5% of its dry weight (green tea, for comparison: 0.5% -1.5%); [45,46]. In an epidemiologic study, the daily intake of 25.9 mg flavonoids (quercetin, kaempferol, myricetin, apigenin and luteolin) was related to a significant decrease of cancer symptoms [47].…”

Antioxidant Activity of Some Nigerian Medicinal Plants Used in Malaria Treatment

“…Total flavonols in each extract was determined using the method of Srivastava et al, 21 Briefly, 1.0 mL of 2% AlCl3 in methanol and 1.5 mL of 5% sodium acetate solutions were added to 12.5 µl of each extract (5 mg/mL in methanol) solution. Each mixture was incubated in water bath at 37˚C for 30 min and absorbance taken at 440 nm.…”