Isolation and Characterization of Soluble Polysaccharides and Insoluble Cell Wall Material of the Pulp from Four Mango (Mangifera indica L.) Cultivars

Abstract

Mature green fruits from monoembryonic (Amélie and Palmer) and polyembryonic (M'Bingue and Tête de Chat) mango cultivars were initiated to ripen with ethylene (10 ppm, 24 h) and then left to reach full ripeness (6 days). After elimination of skin and kernel, pulp was added with HEPES (1/5, w/w) and centrifuged. Soluble polysaccharides were obtained from the supernatant by precipitation with ethanol and freeze-drying. Cell wall material (CWM) was isolated from the pellet by the buffered phenol procedure and further enzymatically destarched. Soluble polysaccharides (∼0.5−0.8%/pulp fresh weight) were essentially highly esterified pectic substances (uronic acids content ∼50−60%; degree of methyl esterification ∼89−97%) and their molecular weights were higher in the polyembryonic cvs. CWM, ∼1%/pulp fresh weight, was mainly built of cellulose (∼20%) and highly esterified pectic substances (uronic acids ∼13−24%; degree of esterification ∼63−73%). Hemicellulosic glucans were more abundant in the monoembryonic (∼9%) than in the polyembryonic (∼4%) cultivars. Keywords: Mango; Mangifera indica L.; cultivars; pulp; soluble polysaccharides; cell wall material

The mango in French-speaking West Africa: varieties and varietal composition of the orchards

“…Its brix rate is higher than Amélie's: 17.2 °Brix for Palmer as opposed to 13.1°Brix for Amélie [10].…”

“…The flesh is a beautiful bright orange. The sugar and acidity levels vary according to the farming conditions, but are generally below those of Floridian cultivars [10]. The taste and smell are easily recognisable.…”

“…The ventral shoulder is short and the apex long, hence the names Bouche longue (Long Mouth) or Nez long (Long nose) (Dadiani, Nundiani). In the publications of Ollé et al [9,10], it is known by the name of Mbengué, the city where it was harvested for these trials. The apex part of the fruit is straight or slightly curved, hence the name 'Sabre' which it is given in certain collections.…”

La mangue en Afrique de l’Ouest francophone : variétés et composition variétale des vergers

“…L'épaule ventrale est courte et l'apex long, d'où les noms de Bouche Longue ou Nez Long (Dadiani, Nundiani). Dans les publications de Ollé et al [9,10], elle est désignée sous le nom de Mbengué, ville où elle avait été récoltée pour ces essais. La partie apicale du fruit est droite ou légèrement recourbée, d'où l'appellation « Sabre » qui lui est donnée dans certaines collections.…”

“…La chair est d'un bel orange vif. Les taux de sucre et d'acidité sont variables selon les conditions de culture mais généralement inférieurs à ceux des cultivars floridiens [10]. Le goût et l'odeur sont aisément reconnaissables.…”