Isolation and Characterization of Sexual Sporulation Mutants of Aspergillus nidulans

Swart, K.; Heemst, Diana van; Slakhorst, M.; Debets, Fons; Heyting, C.

doi:10.1006/fgbi.2001.1266

Cited by 15 publications

(16 citation statements)

References 40 publications

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“…The developmental phenotype strongly resembles that of A. nidulans strains starved for amino acids, which correlates with a reversible block in cleistothecium formation at the so-called micro cleistothecium stage due to derepression of the cross-pathway control (cpc) system Hoffmann et al 2000;Busch et al 2001). This is in agreement with the findings of Swart et al (2001), who isolated mutants with defects in ascospore formation by screening the content of ripe fruit bodies. This represents the first description of a cpc-independent gene of fungal amino acid biosynthesis (Krappmann et al 1999) that influences the developmental program which directs fruit body formation in A. nidulans, probably via crosspathway control.…”

Section: Deletion Of Aroc the Gene Encoding Chorismate Mutase In A supporting

confidence: 83%

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue

Krappmann

Braus

2003

Mol Gen Genomics

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Blaster cassettes are of significant value in functional genomics, as they represent tools with which to inactivate duplicated or homologous genes in an individual organism. We have constructed a novel blaster module which allows repeated gene deletion in the filamentous fungus Aspergillus nidulans. Because bacterial resistance marker cassettes are employed as flanking repeats in direct orientation, the blaster cassette is suited for recombinogenic engineering by ET cloning in Escherichia coli. The functionality of the blaster module was demonstrated by deleting the chorismate mutaseencoding gene aroC of A. nidulans, followed by marker rescue based on mitotic recombination. The resulting aroCD strains are auxotrophic for phenylalanine but not tyrosine, and display a limited capacity for fruit body formation and ascosporogenesis, which depends on the phenylalanine/tyrosine supply. The data support the notion that amino acid status has a strong impact on cleistothecium development in A. nidulans.

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Section: Deletion Of Aroc the Gene Encoding Chorismate Mutase In A supporting

confidence: 83%

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue

Krappmann

Braus

2003

Mol Gen Genomics

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“…Diploid and disomic isolates of N. crassa , originating from nondisjunction at meiosis I, are highly unstable and do not differ in rates and mechanisms of haploidization and mitotic crossing over [51]. Similarly, disomic strains in A. nidulans that resulted from nondisjunction in meiotic metaphase I also were vegetatively unstable [52], [53].…”

Section: Discussionmentioning

confidence: 99%

Meiosis Drives Extraordinary Genome Plasticity in the Haploid Fungal Plant Pathogen Mycosphaerella graminicola

et al. 2009

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Meiosis in the haploid plant-pathogenic fungus Mycosphaerella graminicola results in eight ascospores due to a mitotic division following the two meiotic divisions. The transient diploid phase allows for recombination among homologous chromosomes. However, some chromosomes of M. graminicola lack homologs and do not pair during meiosis. Because these chromosomes are not present universally in the genome of the organism they can be considered to be dispensable. To analyze the meiotic transmission of unequal chromosome numbers, two segregating populations were generated by crossing genetically unrelated parent isolates originating from Algeria and The Netherlands that had pathogenicity towards durum or bread wheat, respectively. Detailed genetic analyses of these progenies using high-density mapping (1793 DArT, 258 AFLP and 25 SSR markers) and graphical genotyping revealed that M. graminicola has up to eight dispensable chromosomes, the highest number reported in filamentous fungi. These chromosomes vary from 0.39 to 0.77 Mb in size, and represent up to 38% of the chromosomal complement. Chromosome numbers among progeny isolates varied widely, with some progeny missing up to three chromosomes, while other strains were disomic for one or more chromosomes. Between 15–20% of the progeny isolates lacked one or more chromosomes that were present in both parents. The two high-density maps showed no recombination of dispensable chromosomes and hence, their meiotic processing may require distributive disjunction, a phenomenon that is rarely observed in fungi. The maps also enabled the identification of individual twin isolates from a single ascus that shared the same missing or doubled chromosomes indicating that the chromosomal polymorphisms were mitotically stable and originated from nondisjunction during the second division and, less frequently, during the first division of fungal meiosis. High genome plasticity could be among the strategies enabling this versatile pathogen to quickly overcome adverse biotic and abiotic conditions in wheat fields.

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“…Genes involved in regulation of cleisothecia formation were mainly identified through A. nidulans mutants defective in distinct stages of sexual development: acleistothecial strains such as Δ nsdD and Δ stuA (Wu and Miller, 1997; Han et al ., 2001), strains that stop the propagation programme after production of Hülle cells such as Δ steA or Δ medA (Clutterbuck, 1969; Vallim et al ., 2000) and strains that are blocked in the maturation of immature cleistothecia (microcleistothecia) such as poorly supplemented amino acid auxotrophs or CPCA overproduction strains (Eckert et al ., 2000; Hoffmann et al ., 2000; Busch et al ., 2001). Additionally, several mutant strains can build cleistothecial shells which are empty (Swart et al ., 2001).…”

Section: Introductionmentioning

confidence: 99%

The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans

Busch

Eckert

Krappmann

et al. 2003

Molecular Microbiology

145

185

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Isolation and Characterization of Sexual Sporulation Mutants of Aspergillus nidulans

Cited by 15 publications

References 40 publications

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue

Meiosis Drives Extraordinary Genome Plasticity in the Haploid Fungal Plant Pathogen Mycosphaerella graminicola

The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans

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