Isolation and characterization of Salmonella spp. from food and food contact surfaces in a chicken processing factory

Ashrafudoulla, Md.; Na, Kyung Won; Byun, Kye-Hwan; Kim, Duk Hyun; Yoon, Jang Won; Mizan, Md. Furkanur Rahaman; Kang, I.; Ha, Sang‐Do

doi:10.1016/j.psj.2021.101234

Cited by 19 publications

(10 citation statements)

References 28 publications

(32 reference statements)

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“…Finally, we found several genes related to the production of biofilms and fimbria, essential abilities needed to persist in the production line. Both biofilms and fimbriae are necessary for attachment and protection against microbicide compounds, like NaOCl, and allow persistence on surfaces [110,111]. Another defense mechanism present in these strains is the CRISPR-Cas system, that consists of space and repeat sequences that vary in size from 21-72 base pairs (bp) and 23-47 bp, respectively, and differ in terms of genes, number, occurrence, and size across genomes and sequences [112].…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

et al. 2021

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Salmonella comprises over 2500 serotypes and foodborne contamination associated with this pathogen remains an important health concern worldwide. During the last decade, a shift in serotype prevalence has occurred as traditionally less prevalent serotypes are increasing in frequency of infections, especially those related to poultry meat contamination. S. Infantis is one of the major emerging serotypes, and these strains commonly display antimicrobial resistance and can persist despite cleaning protocols. Thus, this work aimed to isolate S. Infantis strains from a poultry meat farm in Santiago, Chile and to characterize genetic variations present in them. We determined their genomic and phenotypic profiles at different points along the production line. The results indicate that the strains encompass 853 polymorphic sites (core-SNPs) with isolates differing from one another by 0–347 core SNPs, suggesting variation among them; however, we found discrete correlations with the source of the sample in the production line. Furthermore, the pan-genome was composed of 4854 total gene clusters of which 2618 (53.9%) corresponds to the core-genome and only 181 (3.7%) are unique genes (those present in one particular strain). This preliminary analysis will enrich the surveillance of Salmonella, yet further studies are required to assess their evolution and phylogeny.

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Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

et al. 2021

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“…Motility assays in this study were performed as previously described, with slight modifications [ 4 , 38 ]. This experiment was performed to confirm the effect of quercetin on the two types of motility (swimming and swarming) of S .…”

Section: Methodsmentioning

confidence: 99%

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Roy

Song

Park

2022

Foods

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Quercetin is an active nutraceutical element that is found in a variety of foods, vegetables, fruits, and other products. Due to its antioxidant properties, quercetin is a flexible functional food that has broad protective effects against a wide range of infectious and degenerative disorders. As a result, research is required on food-contact surfaces (rubber (R) and hand gloves (HG)) that can lead to cross-contamination. In this investigation, the inhibitory effects of quercetin, an antioxidant and antibacterial molecule, were investigated at sub-MIC (125; 1/2, 62.5; 1/4, and 31.25; 1/8 MIC, μg/mL) against Salmonella Typhimurium on surfaces. When quercetin (0–125 μg/mL) was observed on R and HG surfaces, the inhibitory effects were 0.09–2.49 and 0.20–2.43 log CFU/cm2, respectively (p < 0.05). The results were confirmed by field emission scanning electron microscopy (FE-SEM), because quercetin inhibited the biofilms by disturbing cell-to-cell connections and inducing cell lysis, resulting in the loss of normal cell morphology, and the motility (swimming and swarming) was significantly different at 1/4 and 1/2 MIC compared to the control. Quercetin significantly (p < 0.05) suppressed the expression levels of virulence and stress response (rpoS, avrA, and hilA) and quorum-sensing (luxS) genes. Our findings imply that plant-derived quercetin could be used as an antibiofilm agent in the food industry to prevent S. Typhimurium biofilm formation.

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“…Biofilm formation in food processing environments can be a source of various kinds of microorganisms, including pathogens [94,95] and toxigenic bacteria [96]. Campylobacteriosis was the most prevalent zoonosis in the EU in 2017, representing nearly 70% of all reported cases [97].…”

Section: Health Aspectsmentioning

confidence: 99%

Electrochemical Control of Biofilm Formation and Approaches to Biofilm Removal

et al. 2022

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This review deals with microbial adhesion to metal-based surfaces and the subsequent biofilm formation, showing that both processes are a serious problem in the food industry, where pathogenic microorganisms released from the biofilm structure may pollute food and related material during their production. Biofilm exhibits an increased resistance toward sanitizers and disinfectants, which complicates the removal or inactivation of microorganisms in these products. In the existing traditional techniques and modern approaches for clean-in-place, electrochemical biofilm control offers promising technology, where surface properties or the reactions taking place on the surface are controlled to delay or prevent cell attachment or to remove microbial cells from the surface. In this overview, biofilm characterization, the classification of bacteria-forming biofilms, the influence of environmental conditions for bacterial attachment to material surfaces, and the evaluation of the role of biofilm morphology are described in detail. Health aspects, biofilm control methods in the food industry, and conventional approaches to biofilm removal are included as well, in order to consider the possibilities and limitations of various electrochemical approaches to biofilm control with respect to potential applications in the food industry.

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Isolation and characterization of Salmonella spp. from food and food contact surfaces in a chicken processing factory

Cited by 19 publications

References 28 publications

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Electrochemical Control of Biofilm Formation and Approaches to Biofilm Removal

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