Isolation and Characterization of Rifampin-Resistant and Streptolydigin-Resistant Mutants of
            <i>Bacillus subtilis</i>
            with Altered Sporulation Properties

Sonenshein, Abraham L.; Cami, B.; Brevet, Jean; Côté, Richard J.

doi:10.1128/jb.120.1.253-265.1974

Cited by 126 publications

(47 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of these cultures then showed that oligosporogenous (Spo'l-) organisms had arisen at a mean frequency of 1.8 x Following mutagenesis of suspensions of SBA52 by treatment with M N N G or EMS as described by Bowring & Morris (1985), attempts were made to select mutant strains displaying all possible combinations of loss of the three phenotypic traits of Spo, Gran and Soh. Sporulation mutants were isolated both by screening visually for their characteristic colony morphology and by selecting for rifampicin resistance (Sonenshein et al 1974). Colonies of mutants defective in granulose synthesis were identified by their non-reaction with I, vapour (Robson et al 1974) and colonies of non-solventogenic mutants were identified by their colour reaction in the tetrazolium blue test procedure.…”

Section: P R O P E R T I E S O F M U T a N T S D E R I V E D From S Tmentioning

confidence: 99%

Stability of solvent production by Clostridium acetobutylicum in continuous culture: strain differences

Woolley

Morris

1990

Journal of Applied Bacteriology

View full text Add to dashboard Cite

Iridium acetohutylicum in continuous culture: strain differences. Journal o/' Applied Bacteriology 69, 71 8-728.Several strains of Clostridium acetobutylicum, including strains ATCC 824 and DSM I73 I, continue to produce solvents during prolonged periods of chemostat culture. In such cultures, dominance is established by asporogenous mutant(s) that retain the ability to produce solvents. Strain NCIB 8052 (which is not identical with ATCC 824) behaved differently in that its chemostat cultures invariably became acidogenic due to ultimate selection of asporogenous mutant(s) unable to produce solvents, incapable of synthesizing granulose, and demonstrating enhanced sensitivity to environmental stresses of various types. These mutants spontaneously reverted. at a low but measurable frequency, to the parental phenotype, indicating that their multiple loss of capacities was the pleiotropic consequence of a lesion in some global regulatory gene. Their resemblance to previously described cls mutants of strain P262 and the possible nature of the affected regulatory gene are discussed. A simple tetrazolium blue plate assay procedure is described which allows visual discrimination between solvent-producing and non-solventogenic colonies of CI. acetobutylicum When Clostridiurn acetobutylicurn NCIB 8052 was grown in chemostat culture under conditions that were unsuitable for solvent production, asporogenous mutant strain@) incapable of producing acetone and butan-1-01 in normal batch culture were quickly selected (Gottschal & Morris 1981). Even when chemostat culture was performed under conditions that were propitious for solvent formation, it was impossible to establish a steady state, and ultimately, irrespective of the nature of the limiting nutrient, solvent production ceased (Stephens et al. 1985). Other strains of CI. acetobutylicurn, e.g. ATCC 824 and DSM 1731, sustained their solvent productivity during prolonged chemostat culture despite the emergence and eventual preponderance of asporogenous mutants (Meinecke et a/. 1984). In contrast, when strain

show abstract

Section: P R O P E R T I E S O F M U T a N T S D E R I V E D From S Tmentioning

confidence: 99%

Stability of solvent production by Clostridium acetobutylicum in continuous culture: strain differences

Woolley

Morris

1990

Journal of Applied Bacteriology

View full text Add to dashboard Cite

show abstract

“…Mutants blocked after stage 0, i.e., stages II, m, and IV, produced a modified RNA polymerase and secreted normal quantities of serine protease and esterase. Sonensheim et al (299) isolated a large number of rifampin and streptolydigin-resistant mutants of B. subtilis, many of which were blocked at stage 0. The authors concluded that only those strains that sporulated less well than their parents were deranged for protease synthesis.…”

Section: Regulation Of Synthesismentioning

confidence: 99%

Extracellular enzyme synthesis in the genus Bacillus.

Priest¹

1977

Bacteriological Reviews

482

164

View full text Add to dashboard Cite

“…Bacteria and phage. B. subtilis 3610 (ATCC 6051) (23) and M021, a rifampin-resistant mutant of strain 168 (3,22), and wild-type phage 0e (23) have been described previously.…”

Section: Methodsmentioning

confidence: 99%

“…Subtilis initiation medium (SIM) was a slight modification of a medium described by Torriani et al (27) and contained: tris(hydroxymethyl)aminomethane-hydrochloride (pH 7.4), 0.1 M; KCl, 10 mg/ml; L-alanine, 0.34 mg/ml; and glucose, 2 mg/ml. Nutrient sporulation medium DSM (22), L broth, L agar, top agar, and media 121A and 121B (23) have been described previously.…”

Section: Methodsmentioning

confidence: 99%

Nucleic acid synthesis and ribonucleic acid polymerase specificity in germinating and outgrowing spores of Bacillus subtilis

Buu¹,

Sonenshein²

1975

J Bacteriol

View full text Add to dashboard Cite

Nucleic acid synthesis was studied during germination and outgrowth of normal spores of Bacillus subtilis, as well as of spores carrying the genome of phage oe. In a system in which development was restricted to the spore-darkening phase, synthesis of ribonucleic acid (RNA), but not deoxyribonucleic acid (DNA), was detected. The extent of RNA synthesis and turnover during this phase was similar for the two types of spores. In a partially darkened population of spores of either type, there was little RNA degradation, whereas there was considerable turnover in a fully darkened population. The DNA-dependent RNA polymerase of dormant or dark spores was not active in vitro with the DNA as template, although a sigma-like factor could be separated from the polymerizing activity by zone centrifugation. Within 40 min after resuspension of dark spores in a medium that allows outgrowth, the enzyme acquired the ability to transcribe the phage DNA efficiently. During outgrowth, both normal and carrier spores synthesized DNA, but in carrier spores this DNA was almost entirely phage specific. The pattern of RNA accumulation in normal spores was in two distinct phases (0 to 60 min and 90 to 180 min). The second phase was absent in outgrowing carrier spores. The burst of phage in carrier spores occurred at 160 to 180 min.Among prokaryotic developmental phenomena, the process of spore germination and outgrowth is particularly interesting to study since it is naturally synchronous and characterized by sequential changes in cell structure and gene expression (7). To study the control of gene expression during outgrowth in a simplified system, we have taken advantage of the finding that infection of sporulating cells of Bacillus subtilis by the virulent bacteriophage oe results not only in lack of phage reproduction but also in trapping of the phage genome within the developing spore (23). The resultant carrier spores can produce phage only during outgrowth. An analogous system has been described for Bacillus cereus (5).Control at the level of ribonucleic acid (RNA) synthesis during outgrowth is suggested by the finding of Armstrong et al. (1, 2) that transcription of the B. subtilis chromosome is ordered in time. They observed the synthesis of 16S and 23S ribosomal RNA before RNA of heterogeneous size (taken to be messenger RNA) began to be synthesized. In addition, others have shown that RNA synthesis is required for successful outgrowth (11), that different RNA species are found in outgrowing spores than in either vegetative or sporulating cells (8), that the appearance of certain bacterial proteins is ordered (9,24,28), and that deoxyribonucleic acid (DNA) replication is delayed during outgrowth (12,20,25,29). As a result, we have placed particular emphasis on comparing nucleic acid synthesis in germinating and outgrowing normal and carrier spores in the hope of finding when the carried phage genome begins to be expressed.Since Oe-induces little or no detectable synthesis of phage-specific proteins or DNA in infected sporulating ...

show abstract

Isolation and Characterization of Rifampin-Resistant and Streptolydigin-Resistant Mutants of Bacillus subtilis with Altered Sporulation Properties

Cited by 126 publications

References 25 publications

Stability of solvent production by Clostridium acetobutylicum in continuous culture: strain differences

Stability of solvent production by Clostridium acetobutylicum in continuous culture: strain differences

Extracellular enzyme synthesis in the genus Bacillus.

Nucleic acid synthesis and ribonucleic acid polymerase specificity in germinating and outgrowing spores of Bacillus subtilis

Contact Info

Product

Resources

About