Isolation and characterization of riboflavin-binding protein from pregnant-rat serum

Muniyappa, K.; Adiga, P. Radhakantha

doi:10.1042/bj1870537

Cited by 30 publications

(6 citation statements)

References 18 publications

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“…The presence of a mammalian carrier protein that facilitates RF uptake across membrane barriers has been alluded to previously (Merrill et al, 1979;Muniyappa and Adiga, 1980a;Visweswariah and Adiga, 1987a,b;Natraj et al, 1988), although its identity remains inconclusive. Given the suggested interspecies homology of this binding protein, human placental trophoblasts (BeWo) were treated with rabbit anti-chicken riboflavin carrier protein (anti-cRCP) to evaluate incidence and dependence of RF internalization on a specific and homologous protein recognized by the antisera.…”

Section: Resultsmentioning

confidence: 99%

Recognition, Cointernalization, and Recycling of an Avian Riboflavin Carrier Protein in Human Placental Trophoblasts

Mason¹,

D'Souza²,

Bareford³

et al. 2006

J Pharmacol Exp Ther

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Absorption of riboflavin (RF) across membrane barriers is essential to cellular oxidation reduction processes. Riboflavin carrier protein (RCP), a 37-kDa secretory protein, is proposed to play an important role in RF absorption, although information on the mammalian ortholog remains unclear. This study alludes to the existence of a mammalian RF carrier protein and further characterizes its carrier role and fate using avian RCP in human placental trophoblast (BeWo), another mammalian cell line, monkey kidney (COS-1), and the avian control, chicken hepatic (LMH/2A) cells. The presence of RCP and its involvement in RF internalization was analyzed by immunofluorescence and immunobinding assays using chicken RCP (cRCP) antibodies. In the presence of anti-cRCP, cellular RF uptake is significantly decreased (5% of control) in BeWo cells.

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Section: Resultsmentioning

confidence: 99%

Recognition, Cointernalization, and Recycling of an Avian Riboflavin Carrier Protein in Human Placental Trophoblasts

Mason¹,

D'Souza²,

Bareford³

et al. 2006

J Pharmacol Exp Ther

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“…There are probably several less-abundant RNA species induced by oestrogen in vertebrate liver, including those coding for the vitamin-binding proteins (Murthy & Adiga, 1978;Muniyappa & Adiga, 1980), which are induced at approx. 1% of the extent of vitellogenin in male chickens after oestrogen stimulation.…”

Section: Rna-excess Hybridization Analysismentioning

confidence: 99%

The preparation and characterization of vitellogenin messenger RNA from rainbow trout (Salmo gairdneri)

Valotaire¹,

Tenniswood²,

Guellec³

et al. 1984

Biochemical Journal

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Agarose-gel electrophoresis of polyadenylated RNA from livers of oestrogen-treated male rainbow trout revealed a major high-Mr species (7200 nucleotides), which is absent from the polyadenylated RNA isolated from hormonally unstimulated male trout liver. Translation in vitro of the RNA from oestrogen-treated males in a mRNA-dependent rabbit reticulocyte lysate produced a protein (Mr 200 000) that could be immunoprecipitated with antibodies against trout serum vitellogenin, but no immunoprecipitable protein was synthesized with RNA from control animals. DNA complementary to the RNA from oestrogen-stimulated and control male trout liver was synthesized and back-hybridized, with R0t1/2 of 3.8 X 10(-2) and 1 X 10(-1) mol X litre-1 X s for RNA from hormone-treated and control animals respectively. The 9% increase in the abundant mRNA after oestrogen stimulation is due to the induction of vitellogenin mRNA.

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“…Esto nos permite deducir que la riboflavina está asociada a la cantidad y calidad de odontoblastos, relacionán-dose directamente con la capacidad que tienen las células ectomesenquimales en diferenciarse en odontoblastos. Durante el embarazo se induce la formación de proteínas transportadoras de riboflavina en varias especies, incluyendo la rata (24) . Al realizar el análisis histológico del efecto de la carencia (0% de vitamina B 2 ), o deficiencia (25% de vitamina B 2 ) sobre el odontoblasto, se encontró que estas células se encuentran muy afectadas con evidentes áreas vacuoladas.…”

Section: Discussionunclassified

Efectos de la deficiencia de riboflavina sobre el desarrollo del tejido dentoalveolar, en ratas*

Delgadillo

Ayala

2012

An Fac med

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ResumenObjetivo: Determinar los cambios histomorfológicos que ocurren en el tejido dentoalveolar de la mandíbula de una progenie de ratas albinas sometidas a dietas con diferentes concentraciones de riboflavina (B 2 ) e iguales concentraciones de los demás nutrientes, durante el periodo de gestación y lactancia, hasta los 28 días de edad. Diseño: Estudio experimental y analítico. Lugar: Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Crías de ratas albinas Holtzman. Intervenciones: Se utilizó 60 crías de ratas, que fueron progenie de 16 ratas albinas Holtzman, conformándose 4 grupos con capacidad de reproducción y con diferentes requerimientos de riboflavina: Grupo A (100% de requerimiento de B 2 ), Grupo B (50% de B 2 ) Grupo C (25% de B 2 ) y Grupo D (0% de B 2 ). Las crías de cada grupo fueron sacrificadas a los 0, 7, 14, 21 y 28 días de nacimiento, y se sometió a estudios histológicos los tejidos dentoalveolares de 60 crías. Principales medidas de resultados: Alteraciones en la talla, peso corporal y el tejido dentoalveolar de las ratas crías. Resultados: Microscópicamente, el estudio reveló que la carencia total de riboflavina en la dieta materna (0% de B 2 ) ocasiona en el tejido dentoalveolar de las crías cambios histomorfológicos, como pérdida de la polaridad nuclear, presencia de microvacuolas y áreas de pseudo-estratificación en ameloblastos y odontoblastos, disminución del número de células del tejido dentoalveolar, aparición de edema en predentina, disminución de las células activas en el caso de fibroblastos y cementoblastos, notable disminución y adelgazamiento de las fibras colágenas y reducción del grosor de las trabeculas óseas. Alteraciones similares pero en menor cuantía se halló en ratas con deficiencia de B 2 . Conclusiones: La carencia total de riboflavina (0%) en la dieta materna de las ratas durante la gestación y lactación provoca en el tejido dentoalveolar de las crías una cantidad y calidad menor de ameloblastos, odontoblastos, fibroblastos y cementoblastos; escasa cantidad de fibras colágenas y adelgazamiento del hueso alveolar. Palabras clave: Manejo nutricional; deficiencia de riboflavina; tejido dentoalveolar. AbstractObjective: To determine histomorphological changes in jaw dentoalveolar tissue in a progeny of albino rats fed with diets containing different concentrations of riboflavin (B 2 ) and equivalent concentrations of other nutrients, during gestation and lactation and until 28 days of birth. Design: Experimental, longitudinal and analytical. Setting: Alberto Guzman Barron Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biologic material: Holtzman albino rats. Interventions: We used 20 Holtzman albino rats (16 females and 4 males) with reproduction capacity and conformed four groups, according to the demands of riboflavin: Group A (100% of B 2 ), Group B (50% of B 2 ), Group C (25% of ...

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Isolation and characterization of riboflavin-binding protein from pregnant-rat serum

Cited by 30 publications

References 18 publications

Recognition, Cointernalization, and Recycling of an Avian Riboflavin Carrier Protein in Human Placental Trophoblasts

Recognition, Cointernalization, and Recycling of an Avian Riboflavin Carrier Protein in Human Placental Trophoblasts

The preparation and characterization of vitellogenin messenger RNA from rainbow trout (Salmo gairdneri)

Efectos de la deficiencia de riboflavina sobre el desarrollo del tejido dentoalveolar, en ratas*

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