Isolation and characterization of rhizobia from nodules of &lt;i&gt;Clitoria ternatea&lt;/i&gt; in Thailand

Duangkhet, Mallika; Chikoti, Yamikani; Thepsukhon, Apiraya; Thapanapongworakul, Pilunthana; Chungopast, Sirinapa; Tajima, Shigeyuki; Nomura, Masatoshi

doi:10.5511/plantbiotechnology.18.0402a

Cited by 9 publications

(7 citation statements)

References 32 publications

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“…strains tested (Zaroug and Munns, 1980b). A more recent study reported the isolation and identification of 11 rhizobial strains from C. ternatea grown in Thailand (Duangkhet et al, 2018). The 16s rDNA phylogenetic analysis revealed that ten of these isolates were Bradyrhizobium elkanii strains while the remaining isolate was a Bradyrhizobium japonicum strain.…”

Section: Historical and Current Applicationsmentioning

confidence: 99%

“…The 16s rDNA phylogenetic analysis revealed that ten of these isolates were Bradyrhizobium elkanii strains while the remaining isolate was a Bradyrhizobium japonicum strain. These C. ternatea B. elkanii strains were shown to promote better plant growth and induce higher nitrogen-fixing capacity than B. elkanii strains isolated from soybean (Duangkhet et al, 2018).…”

Section: Historical and Current Applicationsmentioning

confidence: 99%

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Butterfly Pea (Clitoria ternatea), a Cyclotide-Bearing Plant With Applications in Agriculture and Medicine

et al. 2019

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The perennial leguminous herb Clitoria ternatea (butterfly pea) has attracted significant interest based on its agricultural and medical applications, which range from use as a fodder and nitrogen fixing crop, to applications in food coloring and cosmetics, traditional medicine and as a source of an eco-friendly insecticide. In this article we provide a broad multidisciplinary review that includes descriptions of the physical appearance, distribution, taxonomy, habitat, growth and propagation, phytochemical composition and applications of this plant. Notable amongst its repertoire of chemical components are anthocyanins which give C. ternatea flowers their characteristic blue color, and cyclotides, ultra-stable macrocyclic peptides that are present in all tissues of this plant. The latter are potent insecticidal molecules and are implicated as the bioactive agents in a plant extract used commercially as an insecticide. We include a description of the genetic origin of these peptides, which interestingly involve the co-option of an ancestral albumin gene to produce the cyclotide precursor protein. The biosynthesis step in which the cyclic peptide backbone is formed involves an asparaginyl endopeptidase, of which in C. ternatea is known as butelase-1. This enzyme is highly efficient in peptide ligation and has been the focus of many recent studies on peptide ligation and cyclization for biotechnological applications. The article concludes with some suggestions for future studies on this plant, including the need to explore possible synergies between the various peptidic and non-peptidic phytochemicals.

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Section: Historical and Current Applicationsmentioning

confidence: 99%

Section: Historical and Current Applicationsmentioning

confidence: 99%

Butterfly Pea (Clitoria ternatea), a Cyclotide-Bearing Plant With Applications in Agriculture and Medicine

et al. 2019

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“…Later, nodules were washed with SDW 05 times to remove traces of hydrogen peroxide solution (if any). Nodules were subsequently crushed in 02mL SDW and stored in 1.5mL Eppendorf tubes according to sampling location (Chibeba, Kyei-Boahen, de Fátima Guimarães, Nogueira, & Hungria, 2017;Duangkhet et al, 2018;Palaniappan, Chauhan, Saravanan, Anandham, & Sa, 2010).…”

Section: Legume Nodule Preparationmentioning

confidence: 99%

Isolation and characterization of Rhizobium spp. and Bradyrhizobium spp. from legume nodules

Nguyen

Thao

Khanh

et al. 2022

HCMCOUJS - ENGINEERING AND TECHNOLOGY

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Rhizobia topic has been re-focused in recent years because of new findings on their traits not only as nitrogen-fixing bacteria but also as plant growth-promoting rhizobacteria. When combing rhizobial strains with novel biological carriers (e.g., biochar) for inoculant production, it brings great potential for improving soil health in long-term. Appreciating this trend, this study is designed to isolate and characterize local rhizobial strains from legume fields using the conventional method with some modifications to increase efficiency in rhizobial identification. As a result, 17 rhizobial strains were isolated and classified biochemically that genetic identification outcome confirmed 10 strains belong to 07 different Rhizobium species as R. mayense, R. paknamense, R. pusense, R. miluonense, R. tropici, R. phaseoli, and R. multihospitium while the rest belong to 06 various Bradyrhizobium species as B. elkanii, B. centrosematis, B. guangxiense, B. liaoningense, B. yuanmingense, and B. arachidis. Thermal and saline tolerant tests together with seed germination tests also performed on these rhizobial strains to gain data on their responses to abiotic stresses. By comparing rice and mung bean GI values, we can assess the effectiveness of each rhizobial strains to help seeds at their early germination.

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“…Then nodules were treated for 1 min. in 3 % sodium hypochlorite solution and 10 times washed with sterile deionized water 22 . Disinfected nodules were transferred to a tube with 5 mL sterile deionized water and homogenized using a sterile glass rod.…”

Section: Isolation Of Diazotrophic Microorganismsmentioning

confidence: 99%

Paenibacillus Polymyxa MVY-024: Plant Growth Promoting Bacteria Which is Easy to Apply on an Industrial Scale

Kaziūnienė

Mažylytė

Krasauskas

et al. 2021

Preprint

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In this study, thirteen isolates which were possibly expected to fix nitrogen, were isolated from the soil and pea root nodules and identified by gene analysis of 16S rDNA sequences. Two of these isolates which were able to form endospores and growth on nitrogen free media were selected for spring wheat development research. The isolate Paenibacillus sp. S7 identified as Paenibacillus polymyxa was found to significantly increased amount of ammonium and mineral N amounts in the soil. Furthermore, increased nitrogen accumulation in grain and a chlorophyll index were obtained after wheat treatment. Paenibacillus sp. S7 isolate was selected for further studies and accession number MT900581 and strain name MVY-024 in NCBI nucleotide bank for this isolate was assigned. During cultivation of Paenibacillus sp. MVY-024, sugarcane molasses and yeast extract were determined as the most suitable carbon and nitrogen sources, optimal concentrations were 100 gL-1 and 10 gL-1, respectively. The optimal pH range for cells culturing was between 6.5 and 7.0, optimal air flow rate was 0.4 vvm. It was found that air flow has effect for biomass production and cells endospores formation. After Paenibacillus sp. MVY-024 biomass cultivation optimization, cultured cells number was on average 2.2 × 109 cfu mL-1.

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Isolation and characterization of rhizobia from nodules of <i>Clitoria ternatea</i> in Thailand

Cited by 9 publications

References 32 publications

Butterfly Pea (Clitoria ternatea), a Cyclotide-Bearing Plant With Applications in Agriculture and Medicine

Butterfly Pea (Clitoria ternatea), a Cyclotide-Bearing Plant With Applications in Agriculture and Medicine

Isolation and characterization of Rhizobium spp. and Bradyrhizobium spp. from legume nodules

Paenibacillus Polymyxa MVY-024: Plant Growth Promoting Bacteria Which is Easy to Apply on an Industrial Scale

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