Isolation and characterization of rainbow trout C-reactive protein

Murai, Tetsuya; Kodama, Hiroshi; Naiki, Masaharu; Mikami, Takeshi; Izawa, Hisao

doi:10.1016/0145-305x(90)90007-2

Cited by 51 publications

(16 citation statements)

References 29 publications

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“…In contrast to other methods [17][18][19][20], our approach displayed several APR-related proteins on 2-D gels and it was possible to identify these spots quickly by MALDI-TOF/MS and ESI-MS/MS. The approach demonstrates that comparative proteomics can greatly improve the chances of APP identification.…”

Section: Discussionmentioning

confidence: 94%

Serum acute phase response (APR)-related proteome of loach to trauma

Wang

Peng

2004

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 94%

Serum acute phase response (APR)-related proteome of loach to trauma

Wang

Peng

2004

Fish & Shellfish Immunology

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“…Elevations of levels of C-reactive protein, ceruplasmin, and lysozynle after immunization have been reported in carp and rainbow trout (Siwicki & Studnicka 1986, Murai et al 1990, Rainger & Rowley 1993.…”

Section: Discussionmentioning

confidence: 99%

Leucocyte numbers and phagocytic activity in turbot Scophthalmus maximus following immunization with Vibrio damsela and Pasteurella piscicida O-antigen bacterins

Mm¹,

Figueras²

1995

Dis. Aquat. Org.

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The effects of 2 injected bacterins, Vibrio damsela and Pasteurella piscicida 0-antigen bacterins, on the cell type composition of the peripheral blood and head kidney and on the phagocytic activity of the head kidney leucocytes were studied in turbot Scophthalmus maximus L. Blood and head kidney samples were collected from treated and non-treated turbot at different times in the 168 h period after injection. The number of neutrophils increased markedly in the peripheral blood of P. piscicida 0-antigen injected turbot 24 h after injection, being significantly higher (p < 0.01) than the neutroph~l number in V, damsela 0-antigen lnjected turbot. Forty-eight hours after injection, the numbers of head kidney neutrophils and macrophages in V. damsela and in P. piscicida 0-antigen injected turbot increased significantly (p < 0.05) compared with those in saline-injected turbot while the neutrophils in the peripheral blood decreased in fish treated with each of the vaccines. Turbot neutrophils have peroxidase-positive granules and also showed phagocytic capability. The phagocytic rate of the peripheral blood and head kidney phagocytes was higher in turbot injected with bacterins than in the saline-injected turbot, with the maximum phagocytic activity occurring 4 8 h after injection. Phagocytic activity of phagocytes in peripheral blood, kidney, and spleen was equal. Our data show that the phagocytes of turbot have a low phagocytlc affinity for sheep red blood cells in comparison to what has been reported for rainbow trout. Both V damsela and P. piscicida 0-antigen elicited a similar level of a non-specific immune response.

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“…However, there are still few studies which show this. In rainbow trout, CRP was shown to activate complement and enhance the phagocytosis of V. anguillarum (Nakanishi et al, 1991) and CRP levels increased 3-fold in rainbow trout challenged with this pathogen (Murai et al, 1990).…”

Section: (Iii) Lysozymementioning

confidence: 98%