Isolation and characterization of Pseudoalteromonas sp. from fermented Korean food, as an antagonist to Vibrio harveyi

Morya, Vivek Kumar; Choi, Wooyoung; Kim, Eunki

doi:10.1007/s00253-013-4937-3

Cited by 28 publications

(16 citation statements)

References 24 publications

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“…These qualities have allowed Pseudoalteromonas spp. to be used probiotically to prevent disease in cultured fish and shellfish (22)(23)(24)(25). Their beneficial effects are likely related, at least in part, to their secretion of inhibitors to competing microflora, including fish and shellfish pathogens.…”

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confidence: 99%

Mechanisms for Pseudoalteromonas piscicida-Induced Killing of Vibrios and Other Bacterial Pathogens

Richards

Watson

Needleman

et al. 2017

Appl Environ Microbiol

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is a Gram-negative gammaproteobacterium found in the marine environment. Three strains of pigmented were isolated from seawater and partially characterized by inhibition studies, electron microscopy, and analysis for proteolytic enzymes. Growth inhibition and death occurred around colonies of on lawns of the naturally occurring marine pathogens ,, ,, and Inhibition also occurred on lawns of but not on O157:H7 or serovar Typhimurium. Inhibition was not pH associated, but it may have been related to the secretion of a cysteine protease with strong activity, as detected with a synthetic fluorogenic substrate. This diffusible enzyme was secreted from all three strains. Direct overlay of the colonies with synthetic fluorogenic substrates demonstrated the activity of two aminopeptidase Bs, a trypsin-like serine protease, and enzymes reactive against substrates for cathepsin G-like and caspase 1-like proteases. In seawater cultures, scanning electron microscopy revealed numerous vesicles tethered to the outer surface of and a novel mechanism of direct transfer of these vesicles to Vesicles digested holes in cells, while the congregated around the vibrios in a predatory fashion. This transfer of vesicles and vesicle-associated digestion of holes were not observed in other bacteria, suggesting that vesicle binding may be mediated by host-specific receptors. In conclusion, we show two mechanisms by which inhibits and/or kills competing bacteria, involving the secretion of antimicrobial substances and the direct transfer of digestive vesicles to competing bacteria. species are widespread in nature and reduce competing microflora by the production of antimicrobial compounds. We isolated three strains of and characterized secreted and cell-associated proteolytic enzymes, which may have antimicrobial properties. We identified a second method by which kills It involves the direct transfer of apparently lytic vesicles from the surface of the strains to the surface of cells, with subsequent digestion of holes in the cell walls. Enzymes associated with these vesicles are likely responsible for the digestion of holes in the cell walls. has potential applications in aquaculture and food safety, in control of the formation of biofilms in the environment, and in food processing. These findings may facilitate the probiotic use of to inactivate pathogens and may lead to the isolation of enzymes and other antimicrobial compounds of pharmacological value.

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confidence: 99%

Mechanisms for Pseudoalteromonas piscicida-Induced Killing of Vibrios and Other Bacterial Pathogens

Richards

Watson

Needleman

et al. 2017

Appl Environ Microbiol

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“…For example, probiotics isolated from the crab Scylla serrata used in the larviculture (100,000 liters) of the same crab improved larval survival by inhibiting bacteria of the family Flavobacteriaceae, which caused necrosis in larvae on day 7 of cultivation (Dan and Hamasaki, 2015). Similarly, Morya et al (2014) confirmed that the probiotic activity of the bacterium Pseudoalteromonas sp. in the aquaculture of the crab Portunus trituberculatus improved survival and reduced the pathogen load of Vibrio harveyi.…”

Section: Introductionmentioning

confidence: 79%

Seriola lalandi Larviculture with Probiotic Supplements in Mesocosm Systems

Plaza¹,

Leyton²,

Sayes³

et al. 2017

fisheries-aqua

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Abstract:The present study evaluated the survival of S. lalandi larvae cultivated in a mesocosm system (50 m 3 pool) and inoculated in submerged PVC cages with mesh walls and bottom (450 µm Swiss Nylon). The larvae were fed rotifers and artemia supplemented with the probiotic bacterium Pseudoalteromonas sp. SLP1 and the microalga Nannochloropsis gaditana. Larval survival was evaluated at the stage prior to changing the diet from live feed to pellets. Three months after feeding of the larvae with pellet, the growth (length/weight) and the specific growth rate (SGR) of the larvae supplemented with probiotics were recorded in the initial phase of the culture versus those not treated with probiotics. Higher survival was observed in larvae fed the probiotic supplement than in the control larvae (t-test=4.896, p<0.05). Larval growth in control and treatment increased, with significant differences observed during the three months. The SGR index did not present significant differences between the contol and treatment. These data indicate that after 3 months there were no differences in the growth of fish supplemented with or without probiotics. The results indicate that the addition of the microalga N. gaditana and the probiotic bacterium Pseudoalteromonas sp. SLP1 in the live feed, such as rotifers and artemia, is a good dietary alternative and improves the survival of S. lalandi larvae. The use of probiotics to optimize cultivation systems serves as an example of an ecological remediation alternative that can be applied to other cultures of commercial interest.

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“…Zhou et al [30] Oth:Pseudoalteromonas sp. G-:V. harveryi ATCC 14126 [86] Where probiotics are divided as follows: lb: lactobacilli; bb: Bifidobacterium; oth: other; and pathogens are divided as follows: G+: Gram positive; G−: Gram negative; fng: fungi; prs: parasite; ins: insect. Table 4.…”

Section: Recent Results Of Determining the In Vivo Antimicrobial Assamentioning

confidence: 99%

“…Probiotic candidates have been accessed from very diverse habitats including faeces of breastfed human infants [65,69,80,85,95], faeces of healthy adults [9,15,65,70], faeces of elderly [81], faeces of children [25,96], breast milk [42], human saliva [52], vaginal isolates of healthy women [66,75], various fermented foods or beverages including raw or fermented milk [23,35,44], kefir [97], cheese [51,56,98], whey [99], yogurt [16,41], dahi [100,101], other dairy products [25,36,61], sourdough [102], sausages [17], fermented meat [24], kimchi [10,62], maize [25,59], fermented olives [103], Yerba mate [79], ragi [64], soy sauce [86], soil [104], as well as animal origin including rat faeces [71], geese [68], c...…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effect of Probiotics against Common Pathogens

Fijan¹

2016

Probiotics and Prebiotics in Human Nutrition and Health

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The antimicrobial or antagonistic activity of probiotics is an important property that includes the production of antimicrobial compounds, competitive exclusion of pathogens, enhancement of the intestinal barrier function and others. There are many methods to ascertain probiotic properties, including various in vitro and in vivo methods. The in vivo methods include various modifications of the spot-on lawn assay, agar well diffusion assay "WD" , co-culturing methods, usage of cell lines and others. In many cases in vitro antagonist activity is observed, but in real settings it is not observed. The in vivo methods mainly used are animal models however, their use is being restricted according to the European legislation OJ L . The justification of animal models is also questionable as the results of studies on animals do not predict the same results for humans. The use of replacement alternative methods, for example incorporating human cells and tissues, avoids such confounding variables. Most important studies are doubleblinded randomized clinical trials however, these studies are difficult to perform as it is not easy to achieve uniform conditions. There is a clear need for more elaborate assays that would better represent the complex interactions between the probiotics and the final host. This complex situation is a challenge for scientists.

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Isolation and characterization of Pseudoalteromonas sp. from fermented Korean food, as an antagonist to Vibrio harveyi

Cited by 28 publications

References 24 publications

Mechanisms for Pseudoalteromonas piscicida-Induced Killing of Vibrios and Other Bacterial Pathogens

Mechanisms for Pseudoalteromonas piscicida-Induced Killing of Vibrios and Other Bacterial Pathogens

Seriola lalandi Larviculture with Probiotic Supplements in Mesocosm Systems

Antimicrobial Effect of Probiotics against Common Pathogens

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