Isolation and Characterization of Protein Tyrosine Phosphatase 1B (PTP1B) Inhibitory Polyphenolic Compounds From Dodonaea viscosa and Their Kinetic Analysis

Uddin, Zia; Song, Yeong Hun; Ullah, Mahboob; Li, Zuopeng; Kim, Jeong‐Yoon; Park, Ki Hun

doi:10.3389/fchem.2018.00040

Cited by 15 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, natural PTP-1B inhibitors have also been reported in a variety of dietary substrates such as blueberry, marine sponges, Pueraria lobata root, and Chinese raspberry [58][59][60][61][62]. In actuality, the majority of published studies have focused on flavonoids, phenolics, terpenoids, steroids, and alkaloids, but no research has been published on peptides [60,61,[63][64][65][66][67]. Flavonoids isolated from the medicinal plant Morus alba L. (Moraceae) with four Diels-Alder type adducts (morusalbins A−D) are known for dual functionality in the inhibition of the PTP-1B enzyme (IC 50 value of 1.90−9.67 µM) and α-glucosidase (IC 50 value of 2.29−5.91 µM) [68].…”

Section: Ptp-1b Inhibitorsmentioning

confidence: 99%

Characteristics of Food Protein-Derived Antidiabetic Bioactive Peptides: A Literature Update

Nong

Hsu

2021

IJMS

View full text Add to dashboard Cite

Diabetes, a glucose metabolic disorder, is considered one of the biggest challenges associated with a complex complication of health crises in the modern lifestyle. Inhibition or reduction of the dipeptidyl peptidase IV (DPP-IV), alpha-glucosidase, and protein-tyrosine phosphatase 1B (PTP-1B) enzyme activities or expressions are notably considered as the promising therapeutic strategies for the management of type 2 diabetes (T2D). Various food protein-derived antidiabetic bioactive peptides have been isolated and verified. This review provides an overview of the DPP-IV, PTP-1B, and α-glucosidase inhibitors, and updates on the methods for the discovery of DPP-IV inhibitory peptides released from food-protein hydrolysate. The finding of novel bioactive peptides involves studies about the strategy of separation fractionation, the identification of peptide sequences, and the evaluation of peptide characteristics in vitro, in silico, in situ, and in vivo. The potential of bioactive peptides suggests useful applications in the prevention and management of diabetes. Furthermore, evidence of clinical studies is necessary for the validation of these peptides’ efficiencies before commercial applications.

show abstract

Section: Ptp-1b Inhibitorsmentioning

confidence: 99%

Characteristics of Food Protein-Derived Antidiabetic Bioactive Peptides: A Literature Update

Nong

Hsu

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The PTP1B inhibition assay was done by using the method of Uddin, Z. et al [ 20 ] with slight modification. A PTP1B kit (human, recombinant) was purchased from BIOMOL International LP (Plymouth, PA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Protein tyrosine phosphatase 1B (PTP1B) interacts with the activated IR and with insulin receptor substrate (IRS) proteins. Afterward, it dephosphorylates tyrosine residues on the IR and IRS proteins [ 19 , 20 , 21 ]. Therefore, inhibitors of PTP1B are predicted to be useful for preventing and treating T2DM in vitro.…”

Section: Introductionmentioning

confidence: 99%

Anti-Diabetic Effects of Acankoreagenin from the Leaves of Acanthopanax Gracilistylus Herb in RIN-m5F Cells via Suppression of NF-κB Activation

Man-xia

Yang

Zou³

et al. 2018

Molecules

View full text Add to dashboard Cite

Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. In this manuscript, acankoreagenin isolated from the leaves of Acanthopanax gracilistylus (LAG) is thought to possess excellent anti-diabetic properties. In vitro, anti-diabetic activities were assessed based on the inhibitory activities with α-glucosidase (IC50 13.01 μM), α-amylase (IC50 30.81 μM), and PTP1B (IC50 16.39 μM). Acankoreagenin showed better anti-diabetic effects. Then, an investigation was performed to analyze the insulin secretion effects of the insulin-secreting cell line in RIN-m5F cells. It was found that acankoreagenin could increase the insulin release in RIN-m5F cells. It was also found that acankoreagenin reduced NO production, activity of caspase-3, and the reactive oxygen species levels in the cells injured by processing of cytokines. In western blotting, inactivation of NF-κB signaling was confirmed. Acankoreagenin (20 μM) showed a higher I-κBα expression and lower NF-κB expression than the control group and showed a better expression than the positive control L-NAME (1 mM) (p < 0.05). This study demonstrates the anti-diabetic effects of acankoreagenin in vitro and suggests acankoreagenin might offer therapeutic potential for treating diabetes mellitus.

show abstract

“…The activation of PPAR-γ signi cantly improves insulin sensitivity and combats hyperglycaemia in T2DM 2,26 . Protein tyrosine phosphatase 1B (PTP1B), an enzyme from the PTP family, is primarily involved in insulin and leptin signalling regulation 27 . PTP1B enzyme highly expressed in liver, muscles and fat, down-regulates insulin signalling by catalysing the dephosphorylation of activated insulin receptor and insulin receptor substrate 28,29 .…”

Section: Introductionmentioning

confidence: 99%

Stevia rebaudiana Bertoni leaf extract phytochemicals inhibit the Type 2 Diabetes mellitus receptor targets

Shahid

Azfaralariff

Govender

et al. 2022

Preprint

View full text Add to dashboard Cite

Stevia rebaudiana Betoni (SR) or the candy leaf herbal plant is widely consumed as a non-caloric natural sweetener in substitute of table sugar. The perennial plant is native to South America and is cultivated across the globe. Over the years, there has been an increasing scientific interest in Stevia leaves, which naturally inherent phytochemicals of potent pharmacological properties. Amongst the various pharmacological activities previously evident through in vitro or in vivo studies includes blood sugar regulators, antibacterial, and antiviral properties. Type 2 diabetes mellitus (T2DM), or adult-onset diabetes, is a chronic condition characterized as non-effective insulin production by the pancreas. As a result, blood sugar rises and leads to fatality under an extremely high blood sugar condition. In this study, a library of SR leaf phytochemicals was screened using a comprehensive computational approach for the identification of potential anti-T2DM drug compounds. The in silico inhibitory activities of SR phytochemicals against selected T2DM receptor targets (T2DMrTs) were measured by molecular docking analysis and the best complex, as indicated by the minimum binding affinity and extent of structural interactions, was validated further by molecular dynamics (MD) simulation using the GROMACS webserver. The SR phytochemicals were searched in open-source databases and the corresponding pharmacological properties were predicted by the SwissADME tool. The following proteins were set as the T2DMrTs: α-amylase, α-glucosidase, DPP-4, GLP-1R, PPAR-γ and PTP1B. A total of 177 SR phytochemicals were mined and further screening by molecular docking analysis and pharmacological evaluation identified 19 compounds satisfying the minimum binding energy set at < -7 kcal/mol, Lipinski’s rule of five, and oral bioavailability score. Under all possible receptor-ligand, pair-wise combinations, the complexes showed strong to good stability under a 50 ns MD simulation run. The findings inform new information on SR phytochemicals for T2DM therapeutics and drug development.

show abstract

Isolation and Characterization of Protein Tyrosine Phosphatase 1B (PTP1B) Inhibitory Polyphenolic Compounds From Dodonaea viscosa and Their Kinetic Analysis

Cited by 15 publications

References 24 publications

Characteristics of Food Protein-Derived Antidiabetic Bioactive Peptides: A Literature Update

Characteristics of Food Protein-Derived Antidiabetic Bioactive Peptides: A Literature Update

Anti-Diabetic Effects of Acankoreagenin from the Leaves of Acanthopanax Gracilistylus Herb in RIN-m5F Cells via Suppression of NF-κB Activation

Stevia rebaudiana Bertoni leaf extract phytochemicals inhibit the Type 2 Diabetes mellitus receptor targets

Contact Info

Product

Resources

About