Isolation and characterization of polyphenols-degrading bacteria from olive-mill wastewaters polluted soil

Abstract

In this study 28 bacterial strains, isolated from greenwaters-polluted-soil, were investigated for their ability to grow in presence of phenols added to Mineral Basal Medium (MBM) in aerobic conditions. In particular, three of them were found to be able to use as sole carbon source phenol, cathecol, caffeic acid and ferulic acid with efficiency ranging from 76% (phenol in 5 days, millimolar concentration from 3.7 10 -2 to 9 10 -3 ) to 95% (ferulic acid in 2 days millimolar concentration from 6.8 10 -1 to 3 10 -2 ). For these strains the taxonomic position was studied by amplification and sequencing of 16S rRNA genes. The isolated strains were classified belonging to Arthrobacter sulfureus, Pseudomonas synxantha and Pseudomonas oryzihabitans. Noteworthy, for the first time such Pseudomonas strains have been shown to be able to use polyphenols as the only carbon source in vitro. In fact, to the best of our knowledge, this kind of study were not done on Ps. Synxantha, while it was recently shown the ability of P. oryzihabitans to degrade catechol. These findings may open to new biotechnological applications for the degradation of polyphenols.

Synergistic interaction of phenylpropanoids with antibiotics against bacteria

“…This could possibly be explained by the fact that both these hydroxycinnamic acids are metabolized by the bacteria. The ABTKC for the chlorogenic and caffeic acids was negative, probably because these phenylpropanoids are used by many micro-organisms as carbon sources for their growth (Couteau et al, 2001;Aresta et al, 2010). Based on the time-kill curve studies, it could be concluded that cinnamic, p-coumaric and ferulic acids were the most active in combination with the antibiotics against most of the organisms.…”

Kinetics of biodegradation of diethylketone by Arthrobacter viscosus

“…were used before on the removal of organic compounds with very promising results. Wu et al (2010) used this bacterium to degrade di-n-octyl phthalate, Aresta et al (2010) used Arthrobacter sulfureus isolated from olive-mill wastewater polluted soil to degrade phenol and Quintelas et al (2006Quintelas et al ( , 2010 used Arthrobacter viscosus supported on GAC to remove phenol, o-cresol and chlorophenol from contaminated wastewater.…”

Degradation of Phenolic Compounds in Palm Oil Mill Effluent by Silica‐Immobilized Bacteria in Internal Loop Airlift Bioreactors

“…The synthetic wastewater was prepared from CFMM or deionized water (DI) mixed with specified amounts of phenol or other phenolic compounds. The synthetic wastewater contained m ‐cresol, o ‐cresol, 4‐hydroxybenzoic acid, 3‐methylcatechol, catechol, caffeic acid, and ferulic acid at concentrations from 100 to 500 mg/L to represent the phenolic residues found in industrial wastewater . Another synthetic wastewater sample containing 29 mg/L catechol, 30 mg/L phenol, 47 mg/L caffeic acid, and 82 mg/L ferulic acid was also prepared as described by Aresta et al , who used these phenolic compounds to investigate the activity of polyphenol‐degrading bacteria in an OMW treatment.…”

“…For example, Arthrobacter chlorophenolicus A6 can degrade phenol, 4‐chlorophenol, and 4‐nitrophenol ; the mutual interaction between Bacillu s sp. and Vicia sativa improved phenol degradation from soil ; Arthrobacter sulfureus , Pseudomonas synxantha , and Pseudomonas oryzihabitans can utilize phenol, catechol, caffeic acid, and ferulic acid as carbon sources ; and activated sludge from a wastewater treatment plant is able to degrade phenol, p ‐cresol, and o ‐cresol . However, high concentrations of phenolic compounds are toxic to bacterial cells.…”