Isolation and characterization of polymorphic microsatellite loci from Zelkova schneideriana Hand.-Mazz.

Abstract

ABSTRACT. Zelkova schneideriana is a highly valued hardwood species. An improved technique for isolating codominant compound microsatellite markers was used to develop simple sequence repeat markers for Z. schneideriana. A total of 12 microsatellite loci were identified. Overall, the number of alleles per locus ranged from 8-19, with an average of 11.75. Observed heterozygosity and expected heterozygosity values ranged from 0.109-0.709 and 0.832-0.929, respectively. Polymorphic information content is from 0.803-0.915, with an average of 0.854. These markers will be very important for future research related to the genetic diversity, population structure, patterns of gene flow, and mating system of this species.

‘Chongtian’: A New Zelkova schneideriana cultivar

“…Moreover, because of its poor natural regeneration (Carra et al 2019), the population of Z. schneideriana in the wild has been decreasing (Sun et al 2020). Since 1999, Z. schneideriana has been listed as a national second-grade protected plant species in China (Shao and Zhang 2021).Generally, Z. schneideriana has a large crown (Liu et al 2014), occupying a large planting space. In a limited area, the cultivated density could be highly increased by selecting cultivars that have small canopy diameter.…”

“…Generally, Z. schneideriana has a large crown (Liu et al 2014), occupying a large planting space. In a limited area, the cultivated density could be highly increased by selecting cultivars that have small canopy diameter.…”