Isolation and Characterization of Polyacrylamide-Degrading Bacteria from Dewatered Sludge

Yu, Feng; Fu, Ruimin; Xie, Yun; Wu-ling, Chen

doi:10.3390/ijerph120404214

Cited by 68 publications

(80 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed that the strain could utilize HPAM as their only carbon source and nitrogen source, and the same condition had also been described by Bao et al [18], Wei et al [19] and Yu et al [20] with HPAM removal rates of 33.7%, 48.5% and 56.8%, respectively. Compared with Wang et al [21] degraded hydrolyzed polyacrylamide by the single ozonation and removal rates of TOC and HPAM reached 37.1% and 83.4%, immobilized R2 had higher removal efficiency of TOC and slightly lower one of HPAM.…”

Section: Comparison Between Hpam Biodegradation By Free Strain R2 Andsupporting

confidence: 83%

See 1 more Smart Citation

Biodegradation of partially hydrolyzed polyacrylamide by immobilized bacteria isolated from HPAM‐containing wastewater

Liu

Ren

et al. 2016

Env Prog and Sustain Energy

View full text Add to dashboard Cite

The special function bacteria R2 was screened from hydrolyzed polyacrylamide (HPAM)-containing wastewater that could use HPAM as the sole carbon source and nitrogen source. Through optimizing the condition of HPAM biodegradation, the removal rate of R2 free strain reached to 41.6% when pH was 7, temperature was 358C, inoculation quantity (v/v) was 3% and activation time was 4. In order to overcome the defects of bacteria loss and low microbial utilization rate, microbial immobilization was carried out by mixed bacteria embedding immobilized method. The removal efficiencies of HPAM and TOC were 79.4% and 80.39%, respectively. The immobilized beads was immobilized by embedding medium contained polyvinyl alcohol (PVA) and sodium alginate with concentration of 9% and 3% (w/v), respectively, and the volume ratio of wet bacteria and embedding medium was 1:1 with chemical cross-linked in the saturated boric acid solution. The strain R2 was identified as Pseudomonas aeruginosa UCBPP-PA14 through tests of physiological-biochemical characterization and 16S rDNA sequence. The SEM and HPLC analyses revealed that polyacrylamide was degraded into smaller molecular organism and there was no acrylamide monomer exists after biodegradation. It also could be observed from FT-IR and UV spectrograms that amide group was transformed into carboxyl group completely.

show abstract

Section: Comparison Between Hpam Biodegradation By Free Strain R2 Andsupporting

confidence: 83%

“…and Yu et al . with HPAM removal rates of 33.7%, 48.5% and 56.8%, respectively. Compared with Wang et al .…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of partially hydrolyzed polyacrylamide by immobilized bacteria isolated from HPAM‐containing wastewater

Liu

Ren

et al. 2016

Env Prog and Sustain Energy

View full text Add to dashboard Cite

show abstract

“…High molecular weight polymer chains are degraded by microbes into monomeric and oligomeric units to enable assimilation [43]. Native soil bacteria ( Pseudomonas, Xanthomonas, Rhodococcus, Klebseilla ) have been found to degrade and utilize polyacrylamide (PAM) as sole source of C and N under both aerobic and anaerobic conditions [27, 28, 43, 44]. They secrete PAM-specific extracellular amidase ( amidohydrolase ) enzyme to hydrolyse the amide group into acrylic acid and ammonia, according to Fig.…”

Section: Resultsmentioning

confidence: 99%

Biodegradable water hyacinth cellulose-graft-poly(ammonium acrylate-co-acrylic acid) polymer hydrogel for potential agricultural application

Rop

Mbui

Njomo

et al. 2019

Heliyon

View full text Add to dashboard Cite

Swollen cellulose fibres isolated from water hyacinth were utilized in the synthesis of water hyacinth cellulose-graft-poly(ammonium acrylate-co-acrylic acid) polymer hydrogel (PHG). Acrylic acid (AA) partially neutralized with NH 3 was heterogeneously grafted onto swollen cellulose by radical polymerization reaction using N,N -methylene- bis -acrylamide (MBA) as the cross-linker and ammonium persulphate (APS) as the initiator. The reaction conditions were optimized through assessment of grafting parameters such as grafting cross-linking percentage (GCP), percentage grafting cross-linking efficiency (%GCE) and water absorption tests. Characterization of the copolymer by Fourier Transform Infra-red (FTIR) spectroscopy revealed successful grafting of the monomer onto cellulose. Transmission electron microscopy (TEM) image of acetone-extracted PHG displayed micro-porous structure. The optimized product swelled in distilled water up to 165 times its own dry weight. The swelling was influenced by the pH and presence, nature and concentration of ions. The hydrogel had the capacity to retain moisture in soil, and degradation testing revealed a higher mass loss in cellulose grafted copolymer compared to the copolymer without cellulose. Degradation by soil microbial isolates showed significantly higher (P ≤ 0.05) accumulation of NH 4 + in the cellulose grafted copolymer up to 0.05% (w/v) from 40 to 100 h, relative to similar amounts of copolymer without cellulose. The use of water hyacinth, a notorious weed in Kenyan waters, to produce cellulose-based polymer hydrogels has not been explored and yet, it could form an effective and beneficial way of utilizing this plant. A mechanism of graft polymerization reaction has also been proposed. The synthesized product can be applied in agriculture and other fields where biodegradability and effective utilization of water is essential.

show abstract

“…Previous study has shown that molecular weights above 3 Â 10 3 Da are recalcitrant to microbial mineralization (El-Mamouni et al, 2002). In addition, although Bacillus (Bao et al, 2010), Acinetobacter (Matsuoka et al, 2002), Pseudomonas (Yu et al, 2015), and Clostridium (Ma et al, 2010) grew in the four reactors, their abundances were low. Thus, further studies are required to investigate the mineralization conditions of PAM.…”

Section: Discussionmentioning

confidence: 79%

Cleavage of the main carbon chain backbone of high molecular weight polyacrylamide by aerobic and anaerobic biological treatment

2017

View full text Add to dashboard Cite

High molecular weight partially hydrolyzed polyacrylamide (PAM) can be bio-hydrolyzed on the amide side group, however, solid evidence regarding the biological cleavage of its main carbon chain backbone is limited. In this study, viscometry, flow field-flow fractionation multi-angle light scattering (FFF-MALS), and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) analysis were used to investigate the biodegradability of PAM with a nominal molecular weight of 2 × 10 Da (Da) in two suspended aerobic (25 and 40 °C) and two upflow anaerobic blanket reactors (35 and 55 °C) operated for 470 d under a hydraulic residence time (HRT) of 2 d. Both anaerobic and aerobic biological treatment reduced the viscosity from 2.02 cp in the influent to 1.45-1.60 cp, and reduced the molecular weight of PAM using FFF-MALS from 2.17 × 10 Da to less than one-third its original size. The removals of both the amide group and carbon chain backbone in the PAM molecule were further supported by the FTIR analysis. In comparison with the other conditions, thermophilic anaerobic treatment exhibited higher efficiency for PAM biodegradation. Batch test excluded the influence of temperature on the molecular weight of PAM over the range 25-55 °C, suggesting that cleavage of the main carbon chain backbone was attributed to biological degradation. Our results suggested that high molecular weight PAM was biodegradable, but mineralization did not occur.

show abstract

Isolation and Characterization of Polyacrylamide-Degrading Bacteria from Dewatered Sludge

Cited by 68 publications

References 37 publications

Biodegradation of partially hydrolyzed polyacrylamide by immobilized bacteria isolated from HPAM‐containing wastewater

Biodegradation of partially hydrolyzed polyacrylamide by immobilized bacteria isolated from HPAM‐containing wastewater

Biodegradable water hyacinth cellulose-graft-poly(ammonium acrylate-co-acrylic acid) polymer hydrogel for potential agricultural application

Cleavage of the main carbon chain backbone of high molecular weight polyacrylamide by aerobic and anaerobic biological treatment

Contact Info

Product

Resources

About