Isolation and characterization of plasmacytoid dendritic cells from Flt3 ligand and granulocyte-macrophage colony-stimulating factor–treated mice

Björck, Pia

doi:10.1182/blood.v98.13.3520

Cited by 301 publications

(255 citation statements)

References 29 publications

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“…Phenotypically, the DEC205 ϩ Gr-1 ϩ cells analyzed in this study differ from the recently described mouse plasmacytoid DCs (15)(16)(17) mainly by their lack of B220 expression and the presence of the CD11b marker, although they do retain a common functional characteristic with plasmacytoid DCs regarding the inability to stimulate an allogeneic MLR in vitro (Fig. 3).…”

Section: Discussionmentioning

confidence: 51%

“…Further phenotypic studies revealed the expression of F4/80 and CD11b, suggesting that they present a monocyte/ macrophage phenotype. In addition, results from functional studies showed that the DEC205 ϩ Gr-1 ϩ cells secreted a distinct cytokine profile compared with reports of murine plasmacytoid DCs (15)(16)(17).…”

Section: Discussionmentioning

confidence: 79%

“…2). Overall, these findings suggested that this population might be functionally distinct from the conventional CD11c ϩ DC population or the plasmacytoid B220 ϩ Gr-1 ϩ CD11b Ϫ DCs (15)(16)(17).…”

Section: Phenotypic Characterization Of a Dec205 ϩ Gr-1 ϩ Population mentioning

confidence: 88%

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Identification and Characterization of Novel Bone Marrow Myeloid DEC205+Gr-1+ Cell Subsets That Differentially Express Chemokine and TLRs

Lamb¹,

Capocasale²,

Duffy³

et al. 2007

The Journal of Immunology

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Bone marrow-derived immunomodulatory cytokines impart a critical function in the regulation of innate immune responses and hemopoiesis. However, the source of immunomodulatory cytokines in murine bone marrow and the cellular immune mechanisms that control local cytokine secretion remain poorly defined. Herein, we identified a population of resident murine bone marrow myeloid DEC205+CD11c−B220−Gr1+CD8α−CD11b+ cells that respond to TLR2, TLR4, TLR7, TLR8, and TLR9 agonists as measured by the secretion of proinflammatory and anti-inflammatory cytokines in vitro. Phenotypic and functional analyses revealed that DEC205+CD11b+Gr-1+ bone marrow cells consist of heterogeneous populations of myeloid cells that can be divided into two main cell subsets based on chemokine and TLR gene expression profile. The DEC205+CD11b+Gr-1low cell subset expresses high levels of TLR7 and TLR9 and was the predominant source of IL-6, TNF-α, and IL-12 p70 production following stimulation with the TLR7 and TLR9 agonists CpG and R848, respectively. In contrast, the DEC205+CD11b+Gr-1high cell subset did not respond to CpG and R848 stimulation, which correlated with their lack of TLR7 and TLR9 expression. Similarly, a differential chemokine receptor expression profile was observed with higher expression of CCR1 and CXCR2 found in the DEC205+CD11+Gr-1high cell subset. Thus, we identified a previously uncharacterized population of resident bone marrow cells that may be implicated in the regulation of local immune responses in the bone marrow.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 79%

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Identification and Characterization of Novel Bone Marrow Myeloid DEC205+Gr-1+ Cell Subsets That Differentially Express Chemokine and TLRs

Lamb¹,

Capocasale²,

Duffy³

et al. 2007

The Journal of Immunology

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“…We were unable to use the widely accepted rat granulocyte antibody HIS48, because the IgM isotype control of the HIS48 gave a strong unspecific staining (data not shown). It has been reported that FL induces human and mouse plasmacytoid DCs in vivo and in vitro and that in some mouse strains plasmacytoid DCs express the granulocyte marker Gr-1 [25][26][27][28][29][30]. It is at present unclear whether the MHCII + RK4 + subpopulation of BMDCs represents the rat equivalent of human and mouse plasmacytoid DCs.…”

Section: Discussionmentioning

confidence: 99%

Activation of purified allogeneic CD4+ T cells by rat bone marrow-derived dendritic cells induces concurrent secretion of IFN-γ, IL-4, and IL-10

et al. 2005

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“…In addition, other DC subsets have been implicated in other aspects of antiviral immunity. Most notably, pDCs respond to viruses by producing IFN-␣, which is important for effective antiviral immunity (14,15).…”

mentioning

confidence: 99%

Cutting Edge: Conventional CD8α+ Dendritic Cells Are Preferentially Involved in CTL Priming After Footpad Infection with Herpes Simplex Virus-1

Smith¹,

Belz

Wilson³

et al. 2003

The Journal of Immunology

166

168

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CTL play a major role in immunity to HSV type 1, but little is known about the priming process. In this study, we have examined the class I-restricted presentation of an immunodominant determinant from HSV-1 glycoprotein B after footpad infection. We have found that the only cell types capable of presenting this determinant in draining popliteal lymph nodes within the first 3 days after infection are the CD11c+CD8α+CD45RA− dendritic cells. Given that such class I-restricted presentation is essential for CTL priming, this implies that these conventional CD8α+ dendritic cells are the key subset involved in CTL immunity to this virus.

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Isolation and characterization of plasmacytoid dendritic cells from Flt3 ligand and granulocyte-macrophage colony-stimulating factor–treated mice

Cited by 301 publications

References 29 publications

Identification and Characterization of Novel Bone Marrow Myeloid DEC205+Gr-1+ Cell Subsets That Differentially Express Chemokine and TLRs

Identification and Characterization of Novel Bone Marrow Myeloid DEC205+Gr-1+ Cell Subsets That Differentially Express Chemokine and TLRs

Activation of purified allogeneic CD4+ T cells by rat bone marrow-derived dendritic cells induces concurrent secretion of IFN-γ, IL-4, and IL-10

Cutting Edge: Conventional CD8α+ Dendritic Cells Are Preferentially Involved in CTL Priming After Footpad Infection with Herpes Simplex Virus-1

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