Isolation and characterization of phosphate-solubilizing bacterial species from different crop fields of Salem, Tamil Nadu, India

Abstract

Phosphate-solubilizing bacterial strains (PSB) were isolated from maize, onion, jasmine, and tomato rhizosphere soils from four different localities of Salem (Tamil Nadu) -(i) Ammapalayam, (ii) Attayampatty, (iii) Seeragapadi, and (iv) Ariyanoor. Twelve efficient PSB isolates were selected from the colonies based on their ability to form clear zone on Pikovskaya's agar medium. The isolated PSB released high amount of phosphorus from calcium phosphate. The efficient bacterial strains isolated from rhizosphere soils released high amount of phosphorus. Oligonucleotide primers in RAPD characterization showed genetic variation among the bacterial strains. Amplified fragments showed 40% shared and 20% unique polymorphic bands. The rest 40% was monomorphic bands.

Plant Growth Enhancement using Rhizospheric Halotolerant Phosphate Solubilizing Bacterium Bacillus licheniformis QA1 and Enterobacter asburiae QF11 Isolated from Chenopodium quinoa Willd

“…A bulk of studies have suggested that the presence of PSBs can increase a plant’s tolerance to various abiotic stresses (e.g., drought, salinity, and nutrient deficiency) [ 56 , 57 ]. PSB have been isolated from the rhizosphere of several plants, including wheat [ 58 ], corn [ 59 ] and rice [ 60 ]. However, only few studies have reported on the PSB associated with quinoa roots [ 46 , 61 ].…”

Isolation and characterization of phosphate solubilizing bacteria from agricultural soils for a potential use in cultivating Capsicum frutescens

“…This is due to the fact that about 95% of phosphorus is present in insoluble form in soils, where the plant only can absorb phosphate in soluble form [4]. The phosphorus also forms strong bonds with calcium and magnesium in alkaline soil pH; and with iron and aluminium in acidic soils [5]. Hence, it subsequently causes the mobility of phosphorus in soil to plants become very slow progress.…”

“…However, the focus is mainly on looking for beneficial effect of PSBs to plants, such as some PSBs can convert insoluble forms of nutrients into soluble forms, so the plants can take it up. Subsequently, it is enhancing the growth of plants, including faster ripening of fruits and flowering, also supports the immune system [5]. The phosphorus solubilization ability of bacteria is routinely screened by Pikovskaya (PK) medium that incorporated with tricalcium phosphate (Ca 3 (PO 4 ) 2 ) [10], which is a differential medium.…”