Isolation and characterization of phosphate-solubilizing bacteria from seagrass rhizosphere soil

Ghosh, Upasana; Subhashini, Ponnambalam; Dilipan, E.; Raja, S.; Thangaradjou, T.; Kannan, L.

doi:10.1007/s11802-012-1844-7

Cited by 17 publications

(11 citation statements)

References 33 publications

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“…Results showed that among these four potent isolates, the RK24 strain was the most efficient phosphate solubilizer on PVK medium with SI 4.66 and PSE 366.6. These results are interesting in comparison with results found by Qureshi et al (2012) and Ghosh et al (2012). Modified Pikovskaya's agar containing bromophenol blue as indicator was used, which can improve the clarity and visibility of the yellow-colored halo around colonies.…”

Section: Discussionsupporting

confidence: 80%

“…Thus, intensive screening of phosphate solubilizing bacteria with genetic potential for increased resistance to extreme salt, pH and temperature in arid and semi-arid area, can help to choose the best bacteria for use in production of biological fertilizers (Mehta et al 2001;Ramani 2011). Several studies have reported the isolation of phosphate solubilizing bacteria from rhizospheric soil (Barea et al 1976;Prasanna et al 2011;Ghosh et al 2012;Ambrosini et al 2012). In the present study, cabbage rhizosphere was selected for isolation of phosphate solubilizing bacteria.…”

Section: Discussionmentioning

confidence: 97%

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Screening cabbage rhizosphere as a habitat for isolation of phosphate-solubilizing bacteria

Motamedi¹,

Aalivand²,

Varzi³

et al. 2016

EEB

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Phosphorus plays a major role in plant growth, but due to insoluble complex formation in soil, it is mainly unavailable for plants. Phosphate solubilizing bacteria dissolve phosphate and hence are regarded as biofertilizer. These bacteria are present in different habitats and screening of such habitats can introduce potent phosphate solubilizing bacteria as biologic fertilizer. The aim was finding such isolates from cabbage rhizosphere. Soil samples were enriched and screened using Pikovskaya's agar. Isolates were identified by phenotypic and genotypic methods. Their biological activities were also investigated. Four of these isolates had highest phosphate solubilization index (1.3 to 4.7) and phosphate solubilization efficiency (33.3 to 366.6). Inorganic phosphate solubilizing ability of these strains was 6.74 to 89.1 mg L -1 . The predominant organic acid found was oxalic acid for RK33 isolate. All phosphate solubilizing strains were able to fix nitrogen, while only RK24 and RK33 produced phytase. These isolates were Aeromonas sp., Proteus sp., Proteus mirabilis and Raoultella terrigena. Finally, it can be concluded that cabbage rhizosphere is a potent habitat for finding phosphate solublizing bacteria, which can be applied as biofertilizer, thus avoiding undesirable side effects of chemical fertilizers.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 97%

Screening cabbage rhizosphere as a habitat for isolation of phosphate-solubilizing bacteria

Motamedi¹,

Aalivand²,

Varzi³

et al. 2016

EEB

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“…PSMs able to release phosphorus from the soil minerals play a key role in soil fertility (Wakelin et al 2012) when P availability is low or P demand is high. Most relevant literature pertains to PSB and their potential use for the enhancement of soil fertility (Collavino et al 2010; Ghosh et al 2012). However, a few filamentous nonmycorrhizal fungi are also involved in phosphate solubilization, especially Aspergillus spp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Wang

Sui

et al. 2018

AMB Expr

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Rhizospheric microorganisms can increase phosphorus availability in the soil. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphate-solubilizing bacteria. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents. Among several phosphate-solubilizing fungal isolates screened, strain CS-1 showed the highest phosphorus-solubilization ability. Based on phylogenetic analysis of the internal transcribed spacer region sequence, it was identified as Aspergillus niger. High-performance liquid chromatography analysis revealed that the mechanism of phosphorus solubilization by CS-1 involved the synthesis and secretion of organic acids, mainly oxalic, tartaric, and citric acids. Furthermore, strain CS-1 exhibited other growth-promoting abilities, including efficient potassium release and degradation of crop straw cellulose. These properties help to returning crop residues to the soil, thereby increasing nutrient availability and sustaining organic matter concentration therein. A pot experiment revealed that CS-1 apparently increased the assessed biometric parameters of wheat seedlings, implying the potential of this strain to be developed as a commercial microbial agent. We used Illumina MiSeq sequencing to investigate the microbial community composition in the rhizosphere of uninoculated wheat plants and wheat plants inoculated with the CS-1 strain to obtain insight into the effect of the CS-1 strain inoculation. The data clearly demonstrated that CS-1 significantly reduced the content of pathogenic fungi, including Gibberella, Fusarium, Monographella, Bipolaris, and Volutella, which cause soil-borne diseases in various crops. Strain CS-1 may hence be developed into a microbial agent for plant growth improvement.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0593-4) contains supplementary material, which is available to authorized users.

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“…This could be the first report of such Klebsiella spp., although previous reports described some Klebsiella spp. as phosphate solubilizers (Ghosh et al, 2012;Islama et al, 2007;Chung et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of phosphate solubilizing bacteria (Klebsiella oxytoca) with enhanced tolerant to environmental stress

Walpola¹,

Arunakumara²,

Yoon³

2014

Afr. J. Microbiol. Res.

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The present investigation was aimed at the isolation and characterization of phosphate solubilizing bacteria with enhanced tolerant to environmental stress. The bacterial strain isolated from a metalcontaminated soil collected from abandoned mines was identified as Klebsiella oxytoca according to 16S rRNA analysis. The strain proved the ability to solubilize inorganic phosphate under a wide range of pH (4 to 10), temperature (20 to 40C) and salt concentrations (0 to 7.5% NaCl). However, the maximum phosphate solubilization (615 µg ml -1 ) was recorded when the medium contained glucose (2%) and ammonium sulphate (0.1%), respectively as the source of carbon and nitrogen and NaCl (2.5%) with pH adjusted to 7 at 35C. As revealed by the results of plant growth promoting assays on 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and production of ammonia, hydrogen cyanide (HCN) and indole acitic acid (IAA), the strain was shown to be good plant growth promoter, which was further confirmed by the enhanced growth of mung bean seedlings inoculated with the strain (31.88 and 45.53% higher shoot and root length compared to un-inoculated control). Based on the results, the strain could be identified as an ideal candidate to be included in developing microbial inoculants suit for stress environments.

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Isolation and characterization of phosphate-solubilizing bacteria from seagrass rhizosphere soil

Cited by 17 publications

References 33 publications

Screening cabbage rhizosphere as a habitat for isolation of phosphate-solubilizing bacteria

Screening cabbage rhizosphere as a habitat for isolation of phosphate-solubilizing bacteria

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Isolation and characterization of phosphate solubilizing bacteria (Klebsiella oxytoca) with enhanced tolerant to environmental stress

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