Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga, India

Paul, Dipak; Sinha, Sankar Narayan

doi:10.1016/j.aasci.2016.10.001

Cited by 127 publications

(77 citation statements)

References 30 publications

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“…Under salt stress Phosphate precipitate out and become unavailable for plant growth. PGPR induced P solubilization to make available P is another mechanism of PGPR to alleviate salt stress (Sharma 2013a;Chen et al, 2014;Paul & Sinha, 2017). Many authors have reported that PGPR also secrete phosphate enzyme which solubilize inorganic phosphate which is easily taken up by plant roots and elicit a strong growth promoting effect on plants grown under saline condition (Sharma, 2013b;Kadmiri et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Role of PGPR in the reclamation and revegetation of saline land

Ullah¹,

Bano²

2018

PAK.J.BOT.

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A field experiment was conducted to investigate the effect of PGPR inoculation on growth and yield of maize as well as on reclamation of saline sodic soil of Soil Salinity Research Institute Pindi Bhattian Pakistan, during 2015-16. Seed of maize genotype "Islamabad Gold" were soaked (2-3 h) prior to sowing in the broth culture of 4 bacterial strains i.e. Pseudomonas putida (accession no. KX580766), Pseudomonas fluorescens (accession no. KX644132), Exiguobacterium aurantiacum (accession no. KX580769), Bacillus pumilus (accession no. KX580768) and Lysinibacillus sphaericus (accession no. KX580767). In vitro analysis of bacteria confirmed that they metabolize ACC deaminase, solubilize insoluble phosphate and produce significant quantity of auxin in the presence of L-tryptophan. Inoculation of maize with bacteria along with application of 1L inocula / treatment in the field gave a significant (P = 0.05) increase in germination (76%), leaf chlorophyll (24%), proline (65%), anthocyanin (38%) and soluble sugar content (56%). P. putida inoculation resulted in maximum increase in plant height, leaf area, no of grains cob -1 (459.8), 1000 grain weight (330.9 g) and grain yield (3.25 tha -1 ). P. fluorescens was least effective. The rhizosphere soil analysed after harvesting exhibited significant decrease in electrical conductivity (49%), sodium absorption ratio (98%), and cation exchange capacity (94%) concomitant with a significant increase in organic matter (52%), NO3-N (37%), available P (48%) and K (31%). The highest efficiency of P. putida may be attributed to the maximum ACC deaminase activity, higher production of indole acetic acid and greater potential for Phosphate solubilization. The favorable effects of PGPR were more pronounced in the successive year 2016.

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Section: Discussionmentioning

confidence: 99%

Role of PGPR in the reclamation and revegetation of saline land

Ullah¹,

Bano²

2018

PAK.J.BOT.

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“…Solubilization of inorganic minerals such as phosphate, potassium, and zinc by bacterial strains was carried out using Pikovskaya medium, Aleksandrov medium, and Bunt and Rovira medium only under the nonstress condition. On the basis of the area of solubilization, solubilization index (SI) was calculated using the following formula [20]: The experiment was optimized in a completely randomized design with a factorial arrangement and replicated three times. First, the seeds were surfacesterilized using 2% sodium hypochlorite and placed on each sterilized filter paper (Whatman's No.…”

Section: Mineral Solubilizationmentioning

confidence: 99%

Mitigation of drought stress in rice crop with plant growth‐promoting abiotic stress‐tolerant rice phyllosphere bacteria

Sabarinathan

Gomathy

et al. 2020

J Basic Microbiol

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In the search of effective drought‐alleviating and growth‐promoting phyllosphere bacteria, a total of 44 bacterial isolates were isolated from the leaf surface of drought‐tolerant rice varieties, Mattaikar, Nootripattu, Anna R(4), and PMK3, and screened for their abiotic stress tolerance by subjecting their growth medium to temperature, salinity, and osmotic stress. Only eight isolates were found to grow and proliferate under different abiotic stress conditions. These isolates were identified using 16S ribosomal DNA gene sequence and submitted to the NCBI database. All the bacterial isolates were identified as Bacillus sp., except PB24, which was identified as Staphylococcus sp., and these isolates were further screened for plant growth‐promoting (PGP) traits such as IAA production, GA production, ACC deaminase activity, and exopolysaccharide production under three different osmotic stress conditions adjusted using polyethylene glycol (PEG 6000). Additionally, mineral solubilization was measured under the normal condition. Bacillus endophyticus PB3, Bacillus altitudinis PB46, and Bacillus megaterium PB50 were found to have multifarious PGP traits. Consecutively, the performance of an individual strain to improve the plant growth was investigated under the osmotic stress (25% PEG 6000) and nonstress condition by inoculating them into rice seeds using hydroponics culture. Furthermore, the drought‐alleviating potency of bacterial strains was assessed in the rice plants using pot experiment (−1.2 MPa) through bacterial foliar application during the reproductive stage. Finally, as a result of seed inoculation and foliar spray, the application of B. megaterium PB50 significantly improved the plant growth under osmotic stress, protected plants from physical drought through stomatal closure, and improved carotenoid, total soluble sugars, and total protein content. Metabolites of PB50 were profiled under both stress and nonstress conditions using gas chromatography–mass spectroscopy.

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“…solubilizing microorganisms (PSM) in soil particularly in plant rhizospheres which play an important role in biogeochemical phosphorus cycling in terrestrial and aquatic environments (Paul and Sinha, 2017). Different strains of fungi, bacteria and yeast have been used for large scale phytase production (Naves et al, 2012).…”

Section: Research Articlementioning

confidence: 99%

Screening, Characterization and Physicochemical Optimization of Phosphorus Solubilization Activity of Potential Probiotic Lactobacillus spp.

Arif¹

2018

PVJ

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Present study was designed to develop indigenous phytate hydrolyzing probiotic Lactobacilli to increase phosphorous availability in poultry gut with ultimate goal of efficient and enhanced poultry production. A total of 90 Lactobacilli were isolated from indigenous poultry droppings and homemade fermented milk products. Lactobacilli were screened for phytate hydrolyzing ability and enzyme activities were measured calorimetrically by ammonium molybdate assay. Out of 62 isolates showing phytate hydrolysis, 16 were confirmed by cobalt chloride staining. Out of 16, three isolates (PDP10, PDP24 and FYP38) were identified as Lactobacillus gallinarum, Lactobacillus reutri and Lactobacillus fermentum respectively, and further selected for physico-chemical optimization of phytase production. PDP10

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Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga, India

Cited by 127 publications

References 30 publications

Role of PGPR in the reclamation and revegetation of saline land

Role of PGPR in the reclamation and revegetation of saline land

Mitigation of drought stress in rice crop with plant growth‐promoting abiotic stress‐tolerant rice phyllosphere bacteria

Screening, Characterization and Physicochemical Optimization of Phosphorus Solubilization Activity of Potential Probiotic Lactobacillus spp.

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