Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco

Janati, Walid; Mikou, Karima; Ghadraoui, Lahsen El

doi:10.3389/fmicb.2022.958300

Cited by 5 publications

(5 citation statements)

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“…Plant nutrient models have long demonstrated that slow diffusion of inorganic phosphate (Pi) is a major barrier to P acquisition by plants. Soil P is characterized by its restricted mobility when compared to other important nutrients, such as nitrogen or potassium Janati et al (2022). In the present study, Pikovskaya's media, which utilizes tricalcium phosphate as the only source of phosphorus, was used for the initial screening of the phosphate-solubilizing bacterial strain.…”

Section: Discussionmentioning

confidence: 99%

Production of Organic Acid and Solubilization of Inorganic Phosphate by a Bacterium Isolated From Contaminated Soil

Tuscano,

Gajbhiye

2024

Int. J. Res. Granthaalayah

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Many agricultural soils have significant phosphorus (P) reserves, much of which builds up because of frequent P fertilizer applications. However, roughly 95 to 99% of soil phosphorus is found as insoluble phosphates and is therefore unavailable for plant uptake. The current investigation characterized a bacterial strain that was obtained from contaminated soil and showed the ability to solubilize insoluble inorganic phosphates. An efficient phosphate-solubilizing bacterium was isolated in polluted soil in Mumbai. The phosphate solubilization index of this isolate was assessed using tribasic calcium phosphate-supplemented Pikovskaya’s (PVK) medium. After growing under constant agitation for seven days, the medium pH decreased from 7.0 to 3.5 units. Based on the colony morphology, microscopic analysis, and MALDI-TOF sequencing, the bacterial isolate was identified as Klebsiella pneumoniae. Phosphate solubilization was linked to a pH drop caused by bacterial growth in a medium with glucose as a carbon source. The secretion of organic acids by these phosphate-solubilizing bacteria is responsible for their ability to solubilize inorganic phosphate. GC-MS analysis revealed the presence of carbamic acid, dodecanoic acid, tetra decanoic acid, and trifluoroacetic acid in the culture supernatant. The amount of phosphate solubilized by the bacterium was determined by phosphomolybdate assay and was found to be 667.0 ug/ml which was much higher than the control bacterium S. aureus which was 131.0 ug/ml. To the best of our knowledge, this is the first report mentioning the isolation of phosphate solubilizing bacterium from polluted soil in Mumbai.

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Section: Discussionmentioning

confidence: 99%

Production of Organic Acid and Solubilization of Inorganic Phosphate by a Bacterium Isolated From Contaminated Soil

Tuscano,

Gajbhiye

2024

Int. J. Res. Granthaalayah

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“…The available P portion was determined by a spectrophotometer at 880 nm using the ascorbic acid colourimetric method. The absorbance was measured using standard solutions of KH 2 PO 4 with P concentrations ranging from 0.1 to 1 mg.L −1 ( Janati et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Growth stimulation of two legumes (Vicia faba and Pisum sativum) using phosphate-solubilizing bacteria inoculation

2023

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The application of chemical fertilizers for plant growth and protection is one of the reasons for the environment and ecosystem destruction, thus, sustainable agriculture is gaining popularity in research and among farming communities. Although most soils are high in total phosphorus (P), a large portion is unavailable to plants and regarded as a growth-limiting factor. P-solubilizing bacteria (PSB) exploitation is a newly developed bio-solution for enhancing rhizosphere P availability; however, the effect of these bacteria on soil quality and the different phases of plant growth remains unknown. This study aims to evaluate the impact of five strains of PSB, isolated from legume rhizosphere, on the growth of two plants (Vicia faba and Pisum sativum) and certain soil properties. The efficient strains of PSB used are characterized by the P-solubilization, the ACC deaminase activity, the fixation of N, and the IAA, HCN, and siderophores production. The activity of these bacteria is tested in vitro and in vivo under controlled conditions on the growth of the two plants supplemented with the rock P (RP). According to our findings, all PSBs strains outperformed the control in terms of enhancing the growth of the tested legumes with a percentage ranging from 77.78 to 88.88%, respectively. The results showed that all treatments significantly improved plant parameters like nitrogen- (N) and P-content in the plants (67.50, 23.11%), respectively. Also, an increase in the fresh and dry weights of above- (41.17, 38.57%) and below-ground biomasses (56.6, 42.28%), respectively. Compared to the control, this leads to an increase of 72% in root length, 40.91% in plant dry weight, and 40.07% in fresh weight. Rhizospheric soil in PSBs treatments displayed high levels of N, P, and organic matter. All treatments were found to have significantly higher levels of alkaline phosphatase, basal soil respiration, and β-glucosidase activity than the control. It is concluded that multi-traits PSB can be an alternative for utilizing chemical fertilizers to enhance soil quality and plant growth. Despite the potency of PSBs, its use as a source for the development of sustainable agriculture implies focusing on crop species and adaptation, stress tolerance and climate resilience.

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“…Phosphate solubilizing bacteria were isolated on National Botanical Research in P medium (NBRIP) agar plates [18,19]. To determine the efficiency of P solubilization, the PSB isolates were grown in an NBRIP agar medium supplemented with 5 g.L -1 TCP.…”

Section: Plos Onementioning

confidence: 99%

“…A standard range of KH 2 PO 4 (concentration from 0 to 1 mg.L -1 ) was used to calculate P content. To identify PSB genera, we extended the morphological and biochemical tests conducted by Janati et al (2022) [19]. In this paper, we examine the preliminary screening of PSB isolates and investigate the species affiliation of each potential isolate for phenotypic characterization of phosphate solubilizing bacteria based on growth in a different medium, according to Bergey's textbook (Systematic Bacteriology, 2nd ed., vol.5, eds., 2012).…”

Section: Screening Of Bacterial Plant Growth Promoting Traitsmentioning

confidence: 99%

“…The solubilization halo zone ranged from 1.1 cm to 4.2 cm, and the appearance of the halo zone in all strains was noticed after the 4 th day of incubation. Isolate WJEF8 shows a maximum solubilization index with PSI = 3.84 [19] (Table 5). Furthermore, we noted that an increase in incubation time leads to an improvement in the halo zone size of each isolate, with a correlation between incubation time and halo zone size.…”

Section: Plos Onementioning

confidence: 99%

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Phosphate solubilizing bacteria from soils with varying environmental conditions: Occurrence and function

Janati,

Bouabid,

Mikou

et al. 2023

PLoS ONE

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Phosphate solubilizing bacteria (PSB) is an advantageous way to supply phosphate (P) to plants. The Mediterranean climate of Morocco, especially the low-lying areas, is semi-arid with nutrient-depleted soils in which small-scale, low-income farmers dominate without access to expensive inorganic fertilizers. However, there is not a wide range of PSBs suitable for various agroecological situations. Furthermore, our understanding of the soil and climatic variables that influence their development is limited. This study aims to examine the impacts of specific environmental factors, such as climate and soil, on the abundance, potential, and diversity of PSBs in four agricultural regions of Morocco. To assess the possible impact of these factors on the P solubilization capacity of PSBs and plant growth-promoting (PGP) traits, we analyzed the soil and climate of each sample studied. Similarly, we tested the P solubilization efficiency of the isolates. The bacteria were isolated in a National Botanical Research Institute’s phosphate (NBRIP) agar medium. A total of 51 PSBs were studied in this work. The P-solubilization average of Rock P (RP) and Tricalcium P (TCP) of all strains that were isolated from each of the four regions ranged from 18.69 mg.L-1 to 40.43 mg.L-1 and from 71.71 mg.L-1 to 94.54 mg.L-1, respectively. The PGP traits of the isolated strains are positively correlated with the PSBs abundance and the sample characteristics (soil and climate). The morphological and biochemical characteristics of the strain allowed us to identify around nine different bacterial genera, including Bacillus, Pseudomonas, and Rhizobium. The findings showed that bacterial communities, density, and potency are closely correlated to various edapho-climatic conditions such as temperature, precipitation, soil nutrient status, and soil texture. These findings could be used to improve an effective plant-PSBs system and increase agricultural output by taking into account their specific ecological traits and plant growth mechanisms.

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Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco

Cited by 5 publications

References 74 publications

Production of Organic Acid and Solubilization of Inorganic Phosphate by a Bacterium Isolated From Contaminated Soil

Production of Organic Acid and Solubilization of Inorganic Phosphate by a Bacterium Isolated From Contaminated Soil

Growth stimulation of two legumes (Vicia faba and Pisum sativum) using phosphate-solubilizing bacteria inoculation

Phosphate solubilizing bacteria from soils with varying environmental conditions: Occurrence and function

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