Isolation and Characterization of Phages Infecting<i>Bacillus subtilis</i>

Krasowska, Anna; Biegalska, Anna; Augustyniak, Daria; Łoś, Marcin; Richert, Malwina; Łukaszewicz, Marcin

doi:10.1155/2015/179597

Cited by 35 publications

(23 citation statements)

References 57 publications

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“…In our study, both bacteriophages sustained their optimum activity at neutral pH values, with a decrease in plaque count at acidic or alkaline conditions and total inhibition of phages infectivity at acidic (less than pH 4) and alkaline (more than pH 10) values. Our results are in agreement with [32] who stated that the phage optimum activity should be at neutral pH values. This is attributed to the adsorption of the phage on the host bacterial cell which is affected by pH value, due to charge alteration of protein capsid [33].…”

Section: Discussionsupporting

confidence: 93%

Isolation and Characterization of Two Bacteriophages Infecting Kosakonia sacchari Bacterium Causing Potato Soft Rot Disease

Ea¹,

AlKhzindar²,

Eta³

2018

J Bacteriol Parasitol

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Potato crop is one of the most economically important crops, and is considered as the fourth main food crop in the world. One of the most important serious diseases of potato is bacterial soft rot disease. In our study, soil samples were collected from potato rhizosphere from a potato cultivated field in Giza, Egypt. Two bacteriophages were isolated on Kosakonia sacchari as a host, causing potato soft rot disease. Based on the genome characterization and electron microscopy, the two phages were named as vB_KsaM-C1 belonging to Myoviridae and vB_KsaO-C2 belonging to Microviridae. Both phages sustained their activity more than 20 months with a remarkable decrease in phage titre. In addition both recorded the same TIP at 65°C and showed the optimum activity at pH 7. The DEP of vB_KsaM-C1 was 10-7, while that of phage vB_KsaO-C2 was 10-9. A small scale application of the two isolated bacteriophages on infected potato tubers discs, in the lab, successfully inhibited bacterial soft rot caused by Kosakonia sacchari.

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Section: Discussionsupporting

confidence: 93%

Isolation and Characterization of Two Bacteriophages Infecting Kosakonia sacchari Bacterium Causing Potato Soft Rot Disease

Ea¹,

AlKhzindar²,

Eta³

2018

J Bacteriol Parasitol

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“…Similarly, Krasowska et al . [ 18 ] reported that phage had the highest percent adsorption to the Bacillus host. Due to high activity at the low and high temperature and pH, B. subtilis phage seems to be the best candidate for use in industry.…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, Krasowska et al . [ 18 ] reported B. subtilis phages resistant to the acidic (4.0) and alkaline (9.0 and 10.0) pH. Low pH reportedly affects phage aggregation and reduces their abilities to penetrate the host cells [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bacteriophages with lytic activity against common bacterial pathogens

Shende¹,

Hirpurkar²,

Sannat³

et al. 2017

Vet World

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Aim:Present investigation was conducted to isolate and characterize bacteriophages with lytic activity against common bacterial pathogens.Materials and Methods:A total of 60 samples of animal waste disposal from cattle (42) and buffalo (18) farms were collected from three different strata, i.e., top, mid, and bottom of collection tank. Samples were primarily subjected to rapid detection methods, and then isolation of phage was done by double agar layer method using Bacillus subtilis (BsH) and Escherichia coli (EH) as host system. Phages were characterized on the basis of plaque morphology, temperature, pH susceptibility, and host range.Results:Recovery of phages was higher from dairy cattle farm waste (78.57%) as compared to buffalo farm waste (72.22%) and bottom layer of tank showed maximum recovery. Bacillus subtilis (91%) supported the growth of more phages as compared to E. coli (9%). Three different phage morphotypes were observed each against Bacillus subtilis (BsHR1, BsHR2, and BsHR3) and E. coli (EHR1, EHR2, and EHR3). Mean phage titer of above six phage isolates ranged between 3×1010 and 5×1012 plaque forming units/ml. Viability of phages was by, and large unaffected at 70°C within 2-3 min, and phage isolates were completely inactivated below pH 3 and above 11. Coliphage EHR1 had widest host range followed by BsHR1 and BsHR2 while EHR2, EHR3, and BsHR3 had low lytic activity.Conclusion:It could be concluded from the present study that the Bacillus and Coli phage has wide host range and thus exhibits the potential to be used as drug substitute tool against common bacterial pathogens.

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“…The resistance to environmental stresses assay was performed to investigate the phage application of primary conditions and future prospects (Krasowska et al, 2015). First, phage stability following exposure to varying temperatures and pH was determined.…”

Section: Resistance To Environmental Stressesmentioning

confidence: 99%

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Yang

Liang

Huang³

et al. 2020

Front. Microbiol.

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Accumulating evidence has indicated that the multiple drug resistant Vibrio parahaemolyticus may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 and vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged to the Siphoviridae family with icosahedral head and a long non-contractile tail. The double-stranded DNA genome of phage BA3 was composed of 58648 bp with a GC content of 46.30% while phage CA8 was 58480 bp with an average GC content of 46.42%. In total, 85 putative open reading frames (ORFs) were predicted in the phage BA3 genome while 84 were predicted in that of CA8. The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions. Furthermore, average nucleotide identity analysis, comparative genomic features and phylogenetic analysis revealed that BA3 and CA8 represented different isolates but novel members of the family, Siphoviridae. Regarding the host range of the 61 V. parahaemolyticus isolates, BA3 and CA8 had an infectivity of 8.2 and 36.1%, respectively. Furthermore, ∼100 plaque-forming units (pfu)/cell for phage BA3 and ∼180 pfu/cell for phage CA8 were determined to be the viral load under laboratory growth conditions. Accordingly, the phage-killing assay in vitro revealed that phage CA8 achieved approximately 3.65 log unit reductions. The present results indicate that CA8 is potentially applicable for biological control of multidrug resistant V. parahaemolyticus.

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Isolation and Characterization of Phages InfectingBacillus subtilis

Cited by 35 publications

References 57 publications

Isolation and Characterization of Two Bacteriophages Infecting Kosakonia sacchari Bacterium Causing Potato Soft Rot Disease

Isolation and Characterization of Two Bacteriophages Infecting Kosakonia sacchari Bacterium Causing Potato Soft Rot Disease

Isolation and characterization of bacteriophages with lytic activity against common bacterial pathogens

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

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