Isolation and characterization of petroleum product emulsifying Pseudomonas strains from a generating set fuel tank

Abstract

This study characterized microbial strains isolated from diesel fuel samples collected from the tank of a generating set at the Institute of Research for Development in Pointe-Noire (Congo). Two bacterial isolates (G2 and G3) were distinguished by their color on agar plates and were characterized by their API 20E biochemical profiles and by 16S rRNA gene sequencing. The phenotypic properties of these isolates were consistent with their assignment to the genus Pseudomonas. Comparative 16S rRNA gene sequence analysis demonstrated that the G2 and G3 isolates were close relatives of P. aeruginosa strain GIM 32 and P. aeruginosa strain NV2, respectively, with 97% sequence identity. These two P. aeruginosa strains were able to grow in a mineral salt medium supplemented with 2% diesel fuel or SAE 90 gear oil as the only source of carbon. P. aeruginosa G3 showed faster growth and was able to emulsify diesel fuel (53%), gasoline (90%) and hexane (95%) more strongly than P. aeruginosa G2.

Great Abilities of Shinella zoogloeoides Strain from a Landfarming Soil for Crude Oil Degradation and a Synergy Model for Alginate-Bead-Entrapped Consortium Efficiency

“…This species is therefore worth investigating for hydrocarbon bioremediation. Other isolates obtained in this study belonged to Pseudomonas , a genus renowned for its hydrocarbon degradation capabilities, also via the synthesis of biosurfactants [ 9 , 12 , 39 ]. Particularly, we report the isolation of Pseudomonas guguanensis strain SGPP2.…”

“…Surfactants reduce the surface tension at the oil–water interface, thus encapsulating oil in microdroplets and consequently making it more bioavailable [ 4 ]. Micro-organisms, including bacterial genera Bacillus , Pseudomonas , Rhodococcus , Acinetobacter , Corynebacterium , and Nocardia [ 6 , 9 , 10 , 11 ], are able to synthesize surface active agents called biosurfactants from cheap substrates, such as industrial wastes, or from carbon sources, such as oils [ 4 , 11 ]. Biosurfactants are amphiphilic molecules with hydrophilic and hydrophobic moieties that optimize the microbial uptake of petroleum hydrocarbons by increasing the contact area with the contaminants.…”

“…This is the very first time such a study has been carried out on bacterial isolates from polluted soils originating from Congo. Indeed, although research on microbial degradation of hydrocarbons has expanded in the country over the last few years [ 9 , 14 ], there is no study on microbial entrapment for oil recovery. Usually, in remediation studies, strains are isolated from polluted soils that have not been submitted to any type of remediation.…”

Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou

“…The presence of these species in polluted environments is due to their ability to tolerate and use pollutants as a source of carbon and energy [27]. Most of these strains have already been identified in environments polluted by hydrocarbons [10,[27][28][29]. Liang et al (2014) also identified a strain of Pseudomonas sp.…”

Evaluation of the Bioremediation Potential and Antimicrobial Activity of <i>Pseudomonas</i> and Bacillus Bacteria Isolated from Landfills in Brazzaville, Congo

“…According to the US Environmental Protection Agency's (EPA) National Priority List, 40% of hazardous waste sites are co-contaminated with organic pollutants and heavy metals. Remediation of these sites poses a complex problem due to the mixed nature of the contaminants [10]. The ARIA database (Analyse, Recherche et Information sur les Accidents -Analysis, Research and Information on Accidents), operated by the French Ministry of Ecology, Sustainable Development and Town and Country Planning, has recorded accidental events in France and abroad that have or could have affected public health and safety, agriculture and the environment.…”