Isolation and characterization of permanent cell lines from inner cell mass cells of bovine blastocysts

Stekelenburg-Hamers, A.E.P. van; Achterberg, T. A. E. Van; Rebel, Heggert; Fléchon, J.‐E.; Campbell, Keith; Weima, Sjerp M.; Mummery, Christine L.

doi:10.1002/mrd.1080400408

Cited by 44 publications

(18 citation statements)

References 60 publications

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“…There are several reports about the establishment of bovine ES-like cells from ICM cells [5][6][7][8] and birth of calves after nuclear transfer of these cells [6]. From the results obtained in the present study, if bovine ES-like cells form a clump to some extent after the trypsinization like mammary cells, they would contribute to ICMs of aggregates as well as in mice.…”

Section: Discussionsupporting

confidence: 65%

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In-Vitro Development of Aggregates of Bovine Inner Cell Mass Cells or Bovine Mammary Cells and Putative Tetraploid Embryos Produced by Electrofusion.

Ito

Iwasaki

1999

Journal of Reproduction and Development

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Abstract. The present study was carried out to establish aggregation procedures of bovine inner cell mass (ICM) cells and tetraploid embryos to produce cloned cattle in a different way from nuclear transfer. Bovine two-cell embryos produced by in vitro fertilization were exposed to the electric pulse to fuse 2 blastomeres resulting in tetraploid embryos. Nine (18.8%) of 48 embryos fused by electrofusion developed to blastocyst stage. Three (16.7%) aggregates consisting of two putative tetraploid embryos and one clump of ICM cells developed to blastocyst. And the aggregates which were aggregated with a clump of bovine mammary cells and a clump of ICM cells which were stained with Hoechst also developed to blastocysts. These blastocysts were illuminated by UV, overall or some parts of all ICM of the aggregates treated were observed to luminesce. This raises the possibility that the clumps of the cells can form some parts of ICM of the aggregates. These results indicate that the aggregates of a clump of ICM cells or mammary cells and tetraploid embryos developed to blastocyst stage and that some parts of ICMs of the aggregates were originated from the clump of cells.

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Section: Discussionsupporting

confidence: 65%

“…Furthermore there have been several reports on the establishment of bovine ES cell-like cell lines [5][6][7][8]. Therefore there are some possibilities to produce cloned calves efficiently, if bovine ES cell-like cells can be treated with this aggregation technique using tetraploid embryos.…”

mentioning

confidence: 99%

In-Vitro Development of Aggregates of Bovine Inner Cell Mass Cells or Bovine Mammary Cells and Putative Tetraploid Embryos Produced by Electrofusion.

Ito

Iwasaki

1999

Journal of Reproduction and Development

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“…This result suggests that an increased glucose concentration, instead of increasing glucose metabolism, is detrimental to 5-day-old embryos (morula). Although the role of GH on embryo development is not clear, GH was reported to increase the glucose uptake of the mouse embryo [19]. This effect of GH on glucose transport is due to direct recruitment of the transporters GLUT 1 and GLUT 4 to the plasma membrane [20].…”

Section: Discussionmentioning

confidence: 99%

Stage-Specific Effect of Growth Hormone on Developmental Competence of Bovine Embryos Produced In-Vitro

Iwata¹,

Ohota²,

Hashimoto

et al. 2003

Journal of Reproduction and Development

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Abstract. Many efforts have been made to develop effective culture conditions for the production of bovine blastocysts. Growth hormone (GH) and glucose are known to affect in vitro embryo development. To improve in vitro culture conditions, the culture medium containing fetal calf serum (FCS) or bovine serum albumin (BSA) was supplemented with GH at various periods of development, and the effects of GH on the rate of development and the quality of the blastocysts were studied. Then, starting at the morula stage, the effect of glucose and GH on the rate of development was studied. In all experimental periods, FCS was more effective than BSA at improving the development rate and increasing the cell number of blastocysts. Adding GH to the culture medium between 18 and 48 h after fertilization (1-8 cell stage embryo) did not affect either the rate of blastulation or the cell number regardless of the serum protein (FCS or BSA). From 48 to 120 h after fertilization (5-cell to morula stage) GH increased the cell number of the blastocysts in the presence of BSA, but not in the presence of FCS. From 120 to 192 h after fertilization (morula to blastocyst stage), GH improved the developmental rate and cell number in the presence of FCS, although there was no significant difference when BSA was used instead of FCS as the serum protein. When cows were implanted with blastocysts developed in the presence of GH from the morula stage, their pregnancy rate did not differ from that of the control. Increasing the glucose concentration in the medium from 1.5 mM to 3 mM starting at the morula stage (120 h after fertilization) slightly decreased the rate of development, but on the other hand, decreasing the glucose concentration to 0 mM did not affect either the rate of development or the cell number. Also, then GH had no effect on the developmental rate or the cell number in the absence of glucose. In conclusion, when the medium was supplemented with serum, GH improved embryo development from the morula stage, but an increased concentration of glucose decreased embryo development. Furthermore, GH did not improve the pregnancy rate of blastocysts developed in vitro.

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“…Thomson's group [6] derived human ESC lines from embryos with normal karyotypes and the potential to develop into trophoblasts and three embryonic germ layers. Progress has also been made in other species, including pigs [7][8][9], cattle [7,[10][11][12][13], sheep [14,15], rabbits [16], horses [17], dogs [18], and cats [19], but these studies did not fulfill all characteristics of mESCs. Also, at this time, researchers have failed to derive genuine ESCs from domesticated ungulates such as pigs, cattle, goats, sheep and derivation of germline transmittable chimeric offspring has proven to be elusive in these species.…”

Section: Introductionmentioning

confidence: 99%

Progress, problems and prospects of porcine pluripotent stem cells

Wang¹,

Pei²,

Li³

et al. 2014

Front. Agr. Sci. Eng.

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Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), can differentiate into cells of the three germ layers, suggesting that PSCs have great potential for basic developmental biology research and wide applications for clinical medicine. Genuine ESCs and iPSCs have been derived from mice and rats, but not from livestock such as the pig-an ideal animal model for studying human disease and regenerative medicine due to similarities with human physiologic processes. Efforts to derive porcine ESCs and iPSCs have not yielded high-quality PSCs that can produce chimeras with germline transmission. Thus, exploration of the unique porcine gene regulation network of preimplantation embryonic development may permit optimization of in vitro culture systems for raising porcine PSCs. Here we summarize the recent progress in porcine PSC generation as well as the problems encountered during this progress and we depict prospects for generating porcine naive PSCs.

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Isolation and characterization of permanent cell lines from inner cell mass cells of bovine blastocysts

Cited by 44 publications

References 60 publications

In-Vitro Development of Aggregates of Bovine Inner Cell Mass Cells or Bovine Mammary Cells and Putative Tetraploid Embryos Produced by Electrofusion.

In-Vitro Development of Aggregates of Bovine Inner Cell Mass Cells or Bovine Mammary Cells and Putative Tetraploid Embryos Produced by Electrofusion.

Stage-Specific Effect of Growth Hormone on Developmental Competence of Bovine Embryos Produced In-Vitro

Progress, problems and prospects of porcine pluripotent stem cells

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