Isolation and Characterization of Paraquat-Degrading Extracellular Humus-Reducing Bacteria from Vegetable Field

Wu, Chaoqun; Liu, Jin Kun; Chen, Shan Shan; Deng, Xiao; Li, Qin Fen

doi:10.4028/www.scientific.net/amr.807-809.1026

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…It has been reported that Corynebacterium fascians Dows tolerated extremely high concentrations of bipyridylium ion (10000 mg/L) in dextrose broth up to 4 weeks (Tu and Bollen, 2006). Wu et al (2013) reported that Enterobacter cloacae PQ02 degraded approximately 95% of the initial paraquat dose (50 mg/L) in the presence of extra electron donor anthraquinone-2,6-disulfonic acid (AQDS) and sucrose within 7 days. These carbon sources can easily be utilized by bacteria and accelerate their growth during lag phase.…”

Section: Microbial Degradation Of Paraquatmentioning

confidence: 99%

“…Considering the hazards of paraquat residues on the environment and humans, it is necessary to study paraquat-degrading microorganisms. Microbial degradation is a significant pathway for paraquat breakdown (Mercurio et al, 2014; Wang et al, 2016) and various microorganisms including fungi, bacteria and yeast, have been reported for effective paraquat degradation (Wu et al, 2013; Bai et al, 2014). Anti-oxidative enzyme superoxide dismutase (SOD) contributes toward paraquat tolerance by removing superoxides from living cells, produced during paraquat toxicity (Dos Santos and Silva, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Paraquat Degradation From Contaminated Environments: Current Achievements and Perspectives

et al. 2019

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Paraquat herbicide has served over five decades to control annual and perennial weeds. Despite agricultural benefits, its toxicity to terrestrial and aquatic environments raises serious concerns. Paraquat cannot rapidly degrade in the environment and is adsorbed in clay lattices that require urgent environmental remediation. Advanced oxidation processes (AOPs) and bioaugmentation techniques have been developed for this purpose. Among various techniques, bioremediation is a cost-effective and eco-friendly approach for pesticide-polluted soils. Though several paraquat-degrading microorganisms have been isolated and characterized, studies about degradation pathways, related functional enzymes and genes are indispensable. This review encircles paraquat removal from contaminated environments through adsorption, photocatalyst degradation, AOPs and microbial degradation. To provide in-depth knowledge, the potential role of paraquat degrading microorganisms in contaminated environments is described as well.

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Section: Microbial Degradation Of Paraquatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Paraquat Degradation From Contaminated Environments: Current Achievements and Perspectives

et al. 2019

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“…To understand the mechanism of such enhanced reactivity, we investigated the degradation of paraquat in a humics microbial reducing system. Our results show that paraquat degrade well in humics microbial reducing system with AQDS as the sole electron acceptor, and the addition of organic substrate can enhance the degradation; the degradation rate reached 100% in 5 days of incubation ( Wu et al, 2013a ). However, it is still unclear why organic substrate can enhance the degradation rate of paraquat and what the degradation pathway is.…”

Section: Discussionmentioning

confidence: 74%

“…However, according to Bandara and Kearney (1991) , paraquat degradation was faster in anaerobic soil in comparison to aerobic soil, and organic matter enhanced and facilitated microbial degradation of paraquat under anaerobic conditions. Subsequently, Wu et al (2013a) reported the successful and almost complete anaerobic degradation of paraquat (50 mg/L) by three facultative anaerobic HRMs isolated from vegetable soil. In the presence of the quinone AQDS and sucrose, the herbicide was degraded within 5 days, resulting in a significantly higher degradation rate than those determined under aerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

A Newly Discovered Humic-Reducing Bacterium, Pseudomonas geniculata PQ01, Isolated From Paddy Soil Promotes Paraquat Anaerobic Transformation

Chen

et al. 2020

Front. Microbiol.

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Due to toxicity and persistence of paraquat (a widely used herbicide), eco-friendly remediation approaches to its contamination and effective antidotes to its poisoning have been highly desired and raised increasing concerns. Paraquat degradation was lesser in aerobic soil in comparison with anaerobic soil, and humic-reducing microorganisms (HRMs) play a key role in paraquat anaerobic transformation process. However, the degradation pathways and related mechanisms remain poorly understood. In this study, we investigated the specific interaction mechanisms of the paraquat transformation processes mediated by a humic-reducing strain under anaerobic conditions. A strain of pure culture, designated as PQ01, was successfully isolated from paddy soil using anaerobic enrichment procedure, and identified as Pseudomonas geniculata using phenotypic and phylogenetic analysis. Sucrose, glucose, pyruvate, formic acid, and acetic acid were shown to be favorable electron donors for the reduction of anthrahydroquinone-2,6-disulfonate (AQDS) reduction by PQ01. The strain also had the ability of reducing Fe(III) (hydr)oxides in the presence of sucrose with efficiencies in the order of ferrihydrite > α-FeOOH/γ-FeOOH > γ-Fe 2 O 3 > α-Fe 2 O 3 . In the “PQ01 + paraquat + AQDS + sucrose” system, AQDS reduction and paraquat biotransformation by strain PQ01 occurred simultaneously, and the presence of sucrose significantly enhanced the biotransformation. Specific mechanisms of the electron transfer processes are promoted by both PQ01 and AQDS, and proceed in two aspects: (1) paraquat served as electron donor in the anaerobic reduction of AQDS by strain PQ01; (2) AQDS was reduced by PQ01 anaerobic metabolism to produce AH 2 QDS, which can directly react with paraquat under anaerobic conditions to generate a single crystal compound (molecular formula of the unit structure is C 2 6 H 2 0 N 2 O 8 S 2 ), causing the paraquat to decline dramatically. In conclusion, this main mechanism included the microbial reduction of AQDS to AH 2 QDS, followed by the abiotic reaction between AH 2 QDS and paraquat. This study reported the new characteristics of P. geniculata capable of reducing humics analogs, Fe(III) (hydr)oxides, and paraquat, and proposed a novel electron transformation mechanism of the HRMs’ mediated degradation of organic contaminants.

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“…According to Huang et al (2019) [32], some bacterial and fungal species can degrade paraquat in soils and slurry. These paraquat−degrading microbial strains are Pseudomonas putida [33]; Agrobacterium tumefaciens, Aerobacter aerogenes, Pseudomonas fluorescens, and Bacillus cereus [34]; Enterobacter cloacae PQ02 [35]; and Aeromonas veronii NK67 [36]. They can effectively degrade paraquat and utilize it as a carbon and/or nitrogen source to grow [34,37].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation Capabilities of Paraquat-Degrading Bacteria Immobilized on Nanoceramics

Jindakaraked

Khan

Kajitvichyanukul

2023

Toxics

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The biodegradation of paraquat was investigated using immobilized microbial cells on nanoceramics fabricated from nanoscale kaolinite. Pseudomonas putida and Bacillus subtilis, which degrade paraquat, were immobilized separately on nanoceramics (respectively called ICnc−P and ICnc−B). The attachment of bacteria to nanoceramics resulted from electrostatic force interactions, hydrogen bonding, and covalent bonding (between the cells and the support materials). The initial 10 mg L−1 concentration of paraquat in water was removed by the adsorption process using nanoceramics at 68% and ceramics at 52%, respectively. The immobilized cells on the nanoceramics were able to remove approximately 92% of the paraquat within 10 h, whereas the free cells could only remove 4%. When the paraquat was removed, the cell−immobilized nanoceramics exhibited a significant decrease in dissolved organic nitrogen (DON). ICnc−B was responsible for 34% of DON biodegradation, while ICnc−P was responsible for 22%. Ammonia was identified as the end product of ammonification resulting from paraquat mineralization.

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Isolation and Characterization of Paraquat-Degrading Extracellular Humus-Reducing Bacteria from Vegetable Field

Cited by 7 publications

References 10 publications

Paraquat Degradation From Contaminated Environments: Current Achievements and Perspectives

Paraquat Degradation From Contaminated Environments: Current Achievements and Perspectives

A Newly Discovered Humic-Reducing Bacterium, Pseudomonas geniculata PQ01, Isolated From Paddy Soil Promotes Paraquat Anaerobic Transformation

Biodegradation Capabilities of Paraquat-Degrading Bacteria Immobilized on Nanoceramics

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