Isolation and Characterization of Pancreatic Canine Fetal Cells at the Final Stage of Gestation

Abstract

The incidence of diabetes mellitus in dogs is increasing in recent years, mainly because of genetic and/or environmental factors, including endocrine disorders (like in humans); failure of suitable control of blood sugar levels, which triggers hyperglycemia; glycosuria and weight loss, which demands the development of innovative treatments to cure or treat this complex disease in dogs. The present study established for the first time a protocol to obtain and characterize cells derived from pancreas of canine fetuses. Those fetuses do not have a defined breed and were at the final stage of gestation. The protocol aims to provide morphological data to enable future applications of these cells for therapeutic approaches. In cell culture, pancreatic cells showed a fibroblastlike appearance with a mono-layered growth pattern and were not tumorigenic. They exhibited a positive expression for the pluripotent proliferation markers NANOG and PCNA and expressed PDX1, a transcription factor that is important for activation of the insulin gene promoter. In addition, Tyrosine Hydroxylase-positive (TH+) sympathetic nerve fibers were identified. Histologically, the pancreatic epithelium was developed, pancreatic glands in the fetuses were like those in the parenchyma of postconception dogs and pancreatic islets were unevenly distributed and organized in small clusters along the glands close to the vasculature. Staining with dithizone indicated the presence of insulin in the cells. A large number of beta cells were confirmed by immunofluorescence. In conclusion, the canine fetal pancreas cells could be an alternative and adequate source of cell lineages for stem

Treatment of Canine Type 1 Diabetes Mellitus: The Long Road from Twice Daily Insulin Injection towards Long-Lasting Cell-Based Therapy

“…There are few publications mentioning spontaneous in vivo differentiation (teratoma) using canine iPSCs, but the crucial chimera formation has not been reported yet [94]. Regarding T1DM, the isolation and characterisation of pancreatic canine foetal cells in a 2D model cell culture system were described where the expression of the pluripotent marker NANOG, the proliferation marker PCNA, and the pancreatic transcription factor PDX1 indicated that these cells could be a source of stem cell studies [135].…”

Boric Acid (H3(BO3): Recognize The Molecular Interactions in Solutions

“…Momen Dipol (11) Nama IUPAC (28) Trihidrooksidoboron Asam Borat (15) Nama Lain (10) Sumber (26) Struktur (12) Nama (9) Kegunaan (14) Sifat (1) Asam Boraks (4) Sasolit Asam Ortoborat (27) Borofaks…”