Isolation and Characterization of Novel Chlorella Vulgaris Mutants With Low Chlorophyll and Improved Protein Contents for Food Applications

Abstract

of pigment biosynthesis is a factor that might promote higher protein contents in this species. Moreover, because of their higher protein and lower chlorophyll contents, the MT01 and MT02 strains are likely candidates to be feedstocks for the development of novel, innovative food supplements and foods.

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

“…The nature and concentrations of nitrogen sources, as well as light intensities are important factors affecting pigment and protein accumulation (Benavente-Valdes et al 2016 ; Eze et al 2020 ; Schüler et al 2020 ), growth and biomass productivities (Huo et al 2018 ; Zhu et al 2019 ) by many species of microalgae. The impact of urea supplementation on cell growth in flask cultures has been shown (Fig.…”

“…Although the carotenoid contents were lower than the current report, they were all in agreement in terms of increased productivities under nitrogen-rich and increased light supply conditions. In another study, the carotenoids and protein contents of Chlorella vulgaris(a chlorophyll-deficient mutant) were reportedly enhanced by light irradiance under mixotrophic condition using F2 Guillard medium (Schüler et al 2020 ). Although the authors attributed the higher protein content in the mutant to induction of larger photosynthetic units such as chlorophyll-binding proteins and thylakoid proteins in the presence of light, the nitrate nutrient-sufficient F2 Guillard medium may have contributed to the same effect.…”

Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri

“…Furthermore, cells at high light intensity were, in both cell cycles, richer in protein compared to the low-light-grown cells ( Figure 2 B). This phenomenon might be exploited when a protein-rich algal biomass is required [ 68 , 72 ]. Altogether, this documents the plasticity of the multiple fission lifestyle, which allows the cells to slightly prolong the growth phase in order to be able to reach a critical cell size for starting another reproductive sequence, and to maintain the reproduction potential at the expense of producing slightly smaller daughter cells with altered cell compositions.…”

Technological Feasibility of Couscous-Algae-Supplemented Formulae: Process Description, Nutritional Properties and In Vitro Digestibility

“…The heterotrophic culture was created in a bench-top fermenter in semi-continuous mode (New Brunswick BioFlo ® CelliGen ® 115; Eppendorf AG, Hamburg, Germany), using glucose as the organic carbon source with a C: N ratio of 6.7:1. Chemically random mutagenesis was induced in order to develop chlorophyll-deficient C. vulgaris mutants with different pigmentations [ 15 ]. The biomass was collected aseptically, centrifuged (VWR Micro Star 12, Radnor, PA, USA), spray dried, powdered and stored, until analysis and use.…”

“…Alternative strategies to boost the organoleptic characteristics (appearance and taste) of foods containing algal biomass could help make them more acceptable to consumers [ 8 ]. Examples include the addition of other natural ingredients to improve the flavor and modify the food color, the combination of hetero- and photoautotrophic growth of wild types of microalgae (only possible for some strains) [ 14 ] and chlorophyl-deficient mutants obtained by chemical-induced mutagenesis [ 15 ]. Another approach is the extraction of the microalgae target compounds with the concomitant removal of chlorophyll [ 16 ].…”