Isolation and characterization of novel Fusobacterium nucleatum bacteriophages

Wang, Yue; Liu, Zhitong; Chen, Qian; Yi, Liqi; Zhang, Xu; Cai, Mufeng; Qin, Jinhong; Zhang, Yan; Du, Guanhuan; Hong, Jie; Guo, Xiaokui; Liu, Chang

doi:10.3389/fmicb.2022.945315

Cited by 10 publications

(11 citation statements)

References 65 publications

(102 reference statements)

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“…Despite numerous efforts, the successful isolation of oral bacteriophages has proven challenging. For instance, in a study screening 300 saliva samples from healthy or periodontitis patients and 10 stool samples from colorectal cancer (CRC) patients, only 5 phages were isolated and tested against 11\u00B0 F. nucleatum bacteria ( Wang et al, 2022 ). Similarly, out of 254 test samples (including saliva, teeth, dental plaque, dental effluent, and general sewage), only one lytic phage targeting S. mutans , named SMHBZ8, was identified ( Ben-Zaken et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

SVep1, a temperate phage of human oral commensal Streptococcus vestibularis

Wu,

Zhu,

Yang

et al. 2023

Front. Microbiol.

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IntroductionBacteriophages play a vital role in the human oral microbiome, yet their precise impact on bacterial physiology and microbial communities remains relatively understudied due to the limited isolation and characterization of oral phages. To address this gap, the current study aimed to isolate and characterize novel oral phages.MethodsTo achieve this, oral bacteria were isolated using a culture-omics method from 30 samples collected from healthy individuals. These bacteria were then cultured in three different types of media under both aerobic and anaerobic conditions. The samples were subsequently subjected to full-length 16S rRNA gene sequencing for analysis. Subsequently, we performed the isolation of lytic and lysogenic phages targeting all these bacteria.ResultsIn the initial step, a total of 75 bacterial strains were successfully isolated, representing 30 species and 9 genera. Among these strains, Streptococcus was found to have the highest number of species. Using a full-length 16S rRNA gene similarity threshold of 98.65%, 14 potential novel bacterial species were identified. In the subsequent phase, a temperate phage, which specifically targets the human oral commensal bacterium S. vestibularis strain SVE8, was isolated. The genome of S. vestibularis SVE8 consists of a 1.96-megabase chromosome, along with a 43,492-base pair prophage designated as SVep1. Annotation of SVep1 revealed the presence of 62 open reading frames (ORFs), with the majority of them associated with phage functions. However, it is worth noting that no plaque formation was observed in S. vestibularis SVE8 following lytic induction using mitomycin C. Phage particles were successfully isolated from the supernatant of mitomycin C-treated cultures of S. vestibularis SVE8, and examination using transmission electron microscopy confirmed that SVep1 is a siphovirus. Notably, phylogenetic analysis suggested a common ancestral origin between phage SVep1 and the cos-type phages found in S. thermophilus.DiscussionThe presence of SVep1 may confer immunity to S. vestibularis against infection by related phages and holds potential for being engineered as a genetic tool to regulate oral microbiome homeostasis and oral diseases.

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Section: Discussionmentioning

confidence: 99%

SVep1, a temperate phage of human oral commensal Streptococcus vestibularis

Wu,

Zhu,

Yang

et al. 2023

Front. Microbiol.

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“…nucleatum . This has led to the idea that these bacteria may play a part in the development of oral cancer ( 28 , 29 ). Through a variety of processes, including the production of toxins, enzymes, and signaling molecules, this bacteria may cling to the oral epithelium, cause inflammation, alter the immune system, and encourage carcinogenesis ( 30 ).…”

Section: Oral Bacteria Associated With Osccmentioning

confidence: 99%

Oral microbiota and oral squamous cell carcinoma: a review of their relation and carcinogenic mechanisms

Sukmana,

Saleh,

Najim

et al. 2024

Front. Oncol.

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Oral Squamous Cell Carcinoma (OSCC) is the most common type of head and neck cancer worldwide. Emerging research suggests a strong association between OSCC and the oral microbiota, a diverse community of bacteria, fungi, viruses, and archaea. Pathogenic bacteria, in particular Porphyromonas gingivalis and Fusobacterium nucleatum, have been closely linked to OSCC. Moreover, certain oral fungi, such as Candida albicans, and viruses, like the human papillomavirus, have also been implicated in OSCC. Despite these findings, the precise mechanisms through which the oral microbiota influences OSCC development remain unclear and necessitate further research. This paper provides a comprehensive overview of the oral microbiota and its relationship with OSCC and discusses potential carcinogenic pathways that the oral microbiota may activate or modulate are also discussed.

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“…Therefore, researchers are exploring strategies that selectively manipulate certain bacterial species within the gut microbiota, leading them to investigate bacteriophages. [223,224] Bacteriophages are viruses that have all the common viral properties and exhibit tight host-cell specificity. [225] Previous research mainly focused on the bacteriophages ɸFunu1 and ɸFunu2 associated with F. nucleatum TA B L E  Inorganic-organic hybrid materials against F. nucleatum and F. nucleatum biofilms.…”

Section:  Bacteriophagesmentioning

confidence: 99%

Emerging strategies for combating Fusobacterium nucleatum in colorectal cancer treatment: Systematic review, improvements and future challenges

Liu,

Yu,

Dong

et al. 2023

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Colorectal cancer (CRC) is generally characterized by a high prevalence of Fusobacterium nucleatum (F. nucleatum), a spindle‐shaped, Gram‐negative anaerobe pathogen derived from the oral cavity. This tumor‐resident microorganism has been closely correlated with the occurrence, progression, chemoresistance and immunosuppressive microenvironment of CRC. Furthermore, F. nucleatum can specifically colonize CRC tissues through adhesion on its surface, forming biofilms that are highly resistant to commonly used antibiotics. Accordingly, it is crucial to develop efficacious non‐antibiotic approaches to eradicate F. nucleatum and its biofilms for CRC treatment. In recent years, various antimicrobial strategies, such as natural extracts, inorganic chemicals, organic chemicals, polymers, inorganic‐organic hybrid materials, bacteriophages, probiotics, and vaccines, have been proposed to combat F. nucleatum and F. nucleatum biofilms. This review summarizes the latest advancements in anti‐F. nucleatum research, elucidates the antimicrobial mechanisms employed by these systems, and discusses the benefits and drawbacks of each antimicrobial technology. Additionally, this review also provides an outlook on the antimicrobial specificity, potential clinical implications, challenges, and future improvements of these antimicrobial strategies in the treatment of CRC.

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Isolation and characterization of novel Fusobacterium nucleatum bacteriophages

Cited by 10 publications

References 65 publications

SVep1, a temperate phage of human oral commensal Streptococcus vestibularis

SVep1, a temperate phage of human oral commensal Streptococcus vestibularis

Oral microbiota and oral squamous cell carcinoma: a review of their relation and carcinogenic mechanisms

Emerging strategies for combating Fusobacterium nucleatum in colorectal cancer treatment: Systematic review, improvements and future challenges

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