Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)

Pirseyedi, Seyed Mostafa; Valizadehghan, Sahar; Mardi, Mohsen; Ghaffari, Mohammad Reza; Mahmoodi, Parvaneh; Zahravi, M; Zeinalabedini, Mehrshad; Nekoui, S. M. Khayam

doi:10.3390/ijms11052010

Cited by 67 publications

(49 citation statements)

References 9 publications

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“…This is due to the superior value of the total number of effective alleles, the effective number of patterns/ assay unit and the average number of polymorphic amplicons /assay unit. This finding agrees with previous studies where SRAPs proved to be high effective tools were compared to other marker systems that used each technique separately in pomegranate germplasm, such as, RAPD (Sarkhosh et al, 2006;Narzary et al, 2009), ISSR (Talebi et al, 2005;Narzary et al, 2010), SSR (KoohiDehkordi et al, 2007;Pirseyedi et al, 2010) and SRAP (Soleimani et al, 2012). Other studies in several plants have also confirmed that SRAP is superior information than other methods, for example, RAPD (Liu et al, 2007), ISSR (LiWang et al, 2008) and SSR .…”

Section: Discussionsupporting

confidence: 82%

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Amar¹,

El-Zayat²

2017

Plant Omics

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This study offers the comprehensive analysis of the genetic diversity among six local varieties (Manfalouti; Nab El-Gamal; Hegazy; Wardi; Assuity; Araby) and three international varieties (Wonderful; Marked Bani Rafie; Red Bani Rafie) of pomegranate in Egypt, based on the behavior of ISTR, ISSR and SRAP markers profiles. A set of 24 ISTR, 16 ISSR and 30 SRAP primers combination were compared, concerning exposed the degree of resolution and efficiency of the discriminating capacity technique alongside with the level of genetic polymorphism, to determine which varieties evolved from the others. Considering the results, SRAP assay had superior, more sensitive, higher discrimination capacity and gives much more evidence about the total number of effective alleles (1411), number of polymorphic amplicons (312), PIC (0.94), assay efficiency index (47.04), effective multiples ratio (10.04) and marker index (9.74). Unexpectedly, the ISTR profile demonstrated a significant moderate level of polymorphism among tested genotypes. To further determine the genetic relationships and the distance among varieties, a graphic demonstration of combined UPGMA tree and the PCA analysis did assemble with clear resolution and accurate along with three categories.Our findings confirm that combining different marker system were greatly better and more effective considered an important priority step toward diversity study and characterization. We can point out that, the two-widespread varieties in Egypt Manfalouti, and Nab El-Gamal, formed a high homogeneity in genetic similarity with the Wonderful, as an essential global species for breeding programs, efforts widening the genetic base of pomegranates and the introduction of new genotypes in Egypt.

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Section: Discussionsupporting

confidence: 82%

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Amar¹,

El-Zayat²

2017

Plant Omics

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“…The main differences were in the observed and expected heterozygosities and the PIC values, which indicated that the characteristics of the loci were strongly influenced by the diversity and origin of the samples included in the analysis. These previous studies focused on the diversity of accessions from Tunisia (14,16) and Iran (15). However, similar genetic parameters to the present study were reported by Soriano et al (7) for the PGCT primer set.…”

Section: Genetic Characteristics Of Pomegranatecontrasting

confidence: 50%

“…Interestingly, the values of the genetic parameters calculated in this study were not very similar to those from the previous studies of Hasnaoui et al (14), Pirseyedi et al (15) and Ebrahimi et al (16). The main differences were in the observed and expected heterozygosities and the PIC values, which indicated that the characteristics of the loci were strongly influenced by the diversity and origin of the samples included in the analysis.…”

Section: Genetic Characteristics Of Pomegranatecontrasting

confidence: 49%

“…Although pomegranate is considered as an underused and minor fruit species (11,12), efforts to develop new and highly informative microsatellite molecular markers have been developed in P. granatum (7,(13)(14)(15)(16)(17). Their applicability to P. granatum DNA genotyping procedures and diversity studies has been demonstrated in Iran (15,16), Tunisia (14,17) and Turkey (13).…”

Section: Introductionmentioning

confidence: 99%

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The Genetic, Biochemical, Nutritional and Antimicrobial Characteristics of Pomegranate (Punica granatum L.) Grown in Istria

Višnjevec

Ota

Skrt

et al. 2017

Food Technol. Biotechnol.

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SummaryThis study characterises the genetic variability of local pomegranate (Punica granatum L.) germplasm from the Slovenian and Croatian areas of Istria. The bioactive components and antioxidant and antimicrobial properties of ethanol and water extracts of different parts of pomegranate fruit were also determined, along with their preliminary nutritional characterisation. Twenty-six different genotypes identified with microsatellite analysis indicate the great diversity of pomegranate in Istria. The pomegranate fruit ethanol extracts represent rich sources of phenolic compounds (mean value of the mass fraction in exocarp and mesocarp expressed as gallic acid is 23 and 16 mg/g, respectively). The ethanol extracts of pomegranate exocarp and mesocarp showed the greatest antimicrobial activity against Candida albicans, Candida parapsilosis, Rhodotorula mucilaginosa, Exophiala dermatitidis and Staphylococcus aureus, and the same water extracts against S. aureus and Escherichia coli. To the best of our knowledge, this study represents the first report of the characterisation of pomegranate genetic resources from Istria at different levels, including the molecular, chemical, antimicrobial and nutritional properties.

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“…Compared to the main fruit crops, pomegranate has not been given much attention from geneticists, cytogeneticists or molecular biologists. There have been reports on pomegranate cultivar identification and genetic analysis using molecular markers (Talebi-Baddaf et al, 2003;Sarkhosh et al, 2006;Masoud et al, 2008;Ebrahimi et al, 2010;Hasnaoui et al, 2010;Pirseyedi et al, 2010). However, the studies on effective identification of pomegranate cultivar are limited, since they utilized selected statistical techniques known as cluster analysis to study the banding pattern.…”

Section: Introductionmentioning

confidence: 99%

A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers

Zhang

Tan²,

Cao³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. DNA marker can be used for precise plant cultivar identification. However, DNA markers have often not been used effectively for the identification of plant cultivars due to a lack of an effective analysis strategy. We used a novel strategy for effective identification of plant individuals based on a new way of recording DNA fingerprints of the genotyped plants; a cultivar identification diagram can be manually generated and used as key reference information for quick identification of plant and/ or seed samples. Forty-seven pomegranate varieties popularly cultivated in various provinces of China were subjected to RAPD marker analysis. Using the cultivar identification diagram strategy, they were clearly separated by the fingerprints of 11 RAPD primers. The utility and accuracy of the cultivar identification diagram analysis results were confirmed by the identification of three randomly chosen groups of cultivars among the 47 varieties. A novel strategy for identification of P. granatum cultivars 3033

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Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)

Cited by 67 publications

References 9 publications

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

The Genetic, Biochemical, Nutritional and Antimicrobial Characteristics of Pomegranate (Punica granatum L.) Grown in Istria

A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers

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