Isolation and Characterization of Nontubular Sca-1+Lin− Multipotent Stem/Progenitor Cells from Adult Mouse Kidney

Stem cell options for kidney disease

“…Of note, in the heart Camargo et al [37] have [39] Glycerol-induced ARF (mouse) MSCs Enhanced tubular proliferation [68] IR (rat) Papilla LRCs Proliferation and incorporation [149] IR (mouse) Bone marrow No functional improvement, intrarenal cells are the main source of repopulating cell during repair [22] Folic acid-induced acute tubular injury (mouse) Bone marrow Intrinsic tubular cell proliferation accounts for repair after damage [150] Folic acid-induced acute tubular injury (mouse) Bone marrow 10% incorporation in tubules and G-CSF doubles this rate [151] IR (rat) MSCs Improved renal function and less injury [152] Cisplatin-induced renal failure (mouse) MSCs Accelerated tubular proliferation [153] UUO (mouse) Bone marrow macrophages Reduced renal fibrosis [41] IR (rat) MSCs Improved renal function, increased proliferation and decreased apoptosis [84] IR (rat) rKS56 (S3 segment outgrowth) Replace tubular and improve function [80] Glycerol-induced tubulonecrosis (mouse) Human CD133 + cells Homing and tubular integration [66] UUO (rat) Label-retaining cells (LRC) Proliferates, migrates and transdifferentiates into fibroblast-like cells [27] Cisplatin-induced renal failure (mouse) G-CSF ± M-CSF Improvement in renal function and prevention of renal tubular injury [154] Anti-Thy1.1 GN (rat) MSCs Increased glomerular proliferation and reduction in proteinuria [53] Col4α3 deficiency (mouse) MSCs Prevented loss of peritubular capillaries and reduced fibrosis but no increase in function or survival [24] Col4α3 deficiency (mouse) Bone marrow Partial restoration of expression of the type IV collagen α3 chain, improved histology and function [25] Col4α3 deficiency (mouse) MSCs Improved function and glomerular scarring and interstitial fibrosis reduced [155] UUO (mouse) BM Instertitial BM-derived cells do not contribute significantly to collagen synthesis after damage [74] Adriamycin-nephropathy (mouse) Renal side population Functional amprovement but very low rate of engraftment. [78] IR (rat) Multipotent renal progenitor cells In vivo epithelial differentiation, no difference on renal function [67] Cultured metanephroi (rat) LRCs Integration [81] Glycerol injection (mouse) Human CD24 + CD133+ Tubular regeneration and function improvement [83] IR (mouse) Mice Sca-1 + cells Adopt tubular phenotype …”

“…The conclusion that CD24 + CD133 + cells in the adult human kidney represent a residual population directly derived from this embryonic kidney progenitor population can not be definitively established without lineage tracking, although the similarity of location is provocative. A non-tubular multipotent stem/progenitor cell population was isolated from the adult mouse kidney and characterized as being Sca-1 + Lin − cells [83]. These cells were capable of differentiation into myogenic, adipogenic and neural lineages.…”

Organ-injury-induced reactivation of hemangioblastic precursor cells

“…31 Mice were anesthetized with 100 mg kg À1 ketamine and 10 mg kg À1 xylazine injected intraperitoneally, and a flank incision was made. For unilateral I/R, the left renal pedicle was clamped for 40 min using a vascular clamp (Fine Science Tools Inc., Foster City, CA, USA).…”

“…31 Nuclei were counterstained with hematoxylin. Controls were performed by omitting the primary b-galactosidase antibody or by substituting the primary antibodies with goat IgG isotype.…”

Renal organogenesis

“…37 In addition, a number of studies seeking evidence for postnatal renal stem/progenitor cells have identified non-tubular mesenchymal populations with limited or no tubular potential that may represent such a population. [38][39][40] Outside of the kidney, there is growing evidence that MSC populations frequently exist within the perivasculature where they can express pericyte markers. 41 ( Fig.…”