Isolation and Characterization of Newcastle Disease Virus from Live Bird Markets in Tanzania

Msoffe, Peter L. M.; Chiwanga, Gaspar H.; Cardona, Carol J.; Miller, Patti J.; Suarez, David L.

doi:10.1637/aviandiseases-d-19-00089

Cited by 25 publications

(37 citation statements)

References 31 publications

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“…However, according to the most recent NDV phylogenetic classification using the full-length F gene, these sequences lack branch support and are no longer classified into a separate subgenotype [5]. Genotype V isolates have been previously reported in Tanzania [11,15,23], but most of the Tanzanian sequences deposited in GenBank were significantly shorter than the sequences from this study and therefore were not used. The single strain from Tanzania used in this study is from Mbeya [23], which is approximately 630 km southwest of Morogoro, near the northeast region of Zambia.…”

Section: Discussionmentioning

confidence: 80%

“…Sequences from Uganda and Kenya, countries bordering Northern Tanzania, also clustered with the isolates from Morogoro. Similar genotype V strains seem to have been circulating within these three East African countries and it has been suggested that uncontrolled cross-border trading of live birds might be responsible for these endemic strains [12,15].…”

Section: Discussionmentioning

confidence: 93%

“…With the exception of one strain from Mtwara, all genotype XIII sequences from this study originated from Morogoro, which could justify the genetic homogeneity observed within these isolates. Conversely, a recent study characterizing NDV strains from live bird markets in Tanzania detected nucleotide variability of up to 6% within genotype XIII sequences from Mbeya, Iringa, Arusha and Tanga [15]. This variability is likely due to obtaining the NDV samples in different geographic locations and the origin of the isolates (i.e., from live bird markets).…”

Section: Discussionmentioning

confidence: 93%

“…These chickens may also originate from different geographic locations, and some are sold in live bird markets, where the birds are housed in close proximity and with other avian species. These factors facilitate the circulation and dissemination of various vNDV genotypes, which complicate disease prevention and control measures in these countries [11][12][13][14][15]. To date, NDV isolates from several African countries have been grouped into genotypes I, IV, V, VI, VII, XI, XIII, XIV, XVII, XVIII and XXI, according to the recently updated NDV classification system [5].…”

Section: Introductionmentioning

confidence: 99%

“…To date, NDV isolates from several African countries have been grouped into genotypes I, IV, V, VI, VII, XI, XIII, XIV, XVII, XVIII and XXI, according to the recently updated NDV classification system [5]. In a 2012 study in Tanzania, NDV isolates from chickens in live bird markets representing six different regions were classified as genotype V and XIII [15]. In West and Central Africa, NDV samples belonging to genotypes XIV, XVII, and XVIII were collected between 2006 and 2011 from poultry in live bird markets and backyard and commercial farms [14].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Newcastle Disease Viruses Isolated from Chickens in Tanzania and Ghana

Silva

Aston

Chiwanga

et al. 2020

Viruses

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Newcastle disease (ND) is one of the most challenging infectious diseases affecting poultry production in Africa, causing major economic losses. To date, Newcastle disease virus isolates from several African countries have been grouped into class II NDV genotypes I, IV, V, VI, VII, XI, XIII, XIV, XVII, XVIII and XXI. Although ND is endemic in many African countries, information on circulating genotypes is still scarce. In Tanzania, outbreaks with genotypes V and XIII have been reported. In West and Central Africa, genotypes XIV, XVII, and XVIII are the most predominant. To investigate other genotypes circulating in Tanzania and Ghana, we performed molecular genotyping on isolates from Tanzania and Ghana using the MinION, a third-generation portable sequencing device from Oxford Nanopore Technologies. Using the MinION, we successfully sequenced the NDV F gene hypervariable region of 24 isolates from Tanzania and four samples from Ghana. In Tanzania, genotypes V, VII and XIII were detected. All isolates from Ghana belonged to genotype XVIII. The data obtained in this study reflect the genetic diversity of NDV in Africa and highlight the importance of surveillance for monitoring the distribution of NDV genotypes and viral evolution.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Characterization of Newcastle Disease Viruses Isolated from Chickens in Tanzania and Ghana

Silva

Aston

Chiwanga

et al. 2020

Viruses

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Epidemiology of Newcastle disease in poultry in Africa: systematic review and meta-analysis

Mngumi

Mpenda

Buza

2022

Trop Anim Health Prod

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Molecular characterization of Newcastle disease virus obtained from Mawenzi live bird market in Morogoro, Tanzania in 2020–2021

Tsaxra,

Abolnik,

Kelly

et al. 2023

Braz J Microbiol

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Newcastle disease (ND) is among the most important poultry diseases worldwide. It is the major threat to poultry production in Africa and causes major economic losses for both local and commercial chickens. To date, half of ND class II genotypes have been reported in Africa (I, IV, V, VI, VII, XI, XIII, XIV, XVII, XVIII, and XXI). The information on the circulating NDV genotypes is still scarce despite the endemic nature of ND in most countries on the African continent.A total of 659 oro-cloacal swabs were collected from local chickens in Mawenzi live bird market located in Morogoro, Tanzania, between June 2020 and May 2021. Newcastle disease virus was detected by using reverse transcription real-time polymerase chain reaction (RT-qPCR) and conventional PCR followed by sequencing of PCR products. The prevalence of NDV in the surveilled live bird markets was 23.5%. Sequencing and phylogenetic analysis revealed the presence of sub-genotype VII.2. The detected sub-genotype VII.2 has phylogenetic links to Zambian NDV strains implying a Southeast dissemination of the virus, considering that it was first detected in Mozambique. This study underscores the need of active NDV surveillance to determine the distribution of this NDV genotype in the country and monitor its spread and contribution to the emergence of new ND viruses.

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Isolation and Characterization of Newcastle Disease Virus from Live Bird Markets in Tanzania

Cited by 25 publications

References 31 publications

Molecular Characterization of Newcastle Disease Viruses Isolated from Chickens in Tanzania and Ghana

Molecular Characterization of Newcastle Disease Viruses Isolated from Chickens in Tanzania and Ghana

Epidemiology of Newcastle disease in poultry in Africa: systematic review and meta-analysis

Molecular characterization of Newcastle disease virus obtained from Mawenzi live bird market in Morogoro, Tanzania in 2020–2021

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