Isolation and Characterization of New Cyclohexylacetic Acid-Degrading Bacteria

Abstract

Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A selected strain, KUA-1, which is the first reported Gram-negative organism capable of growth on cyclohexylacetic acid, was identified as a Cupriavidus metallidurans, based on morphologic and physiologic characteristics and its 16S rRNA gene sequence. Metabolite analysis by HPLC-MS indicated that 1-cyclohexenylacetic acid is an intermediate of cyclohexaneacetic acid metabolism in strain KUA-1.

Genome Sequence Analysis of the Naphthenic Acid Degrading and Metal Resistant Bacterium Cupriavidus gilardii CR3

“…Another representative of this taxon, C . metallidurans strain KUA-1, can degrade the standard reference naphthenic acid, cyclohexylacetic acid (CHAA) [ 18 ].…”

“…To our knowledge, there is no pathway associated with degradation of this naphthenic acid in Cupriavidus . Although strain KUA-1 has been reported to degrade cyclohexylacetic acid [ 18 ], the degradation pathway and its substrate versatility remain largely unknown.…”

Genetic manipulation tools for Dietzia spp.

“…2007), have been shown to be degraded. A Dietzia strain isolated from enrichment cultures was found to be one of the few micro‐organisms able to utilize cyclohexylacetic acid (Iwaki et al. 2008).…”

“…Aromatic compounds, such as naphthalene (Brito et al 2006;von der Weid et al 2007), phenanthrene (Al-Awadhi et al 2007), benzoate (Maeda et al 1998), toluene, carbazole and quinoline (von der Weid et al 2007), have been shown to be degraded. A Dietzia strain isolated from enrichment cultures was found to be one of the few micro-organisms able to utilize cyclohexylacetic acid (Iwaki et al 2008).…”

Isolamento e identificação de bactérias com potencial para realizar biorremediação de cobre.

“…Obs. :1 -C. necator, 2 -C. oxalaticus, 3 -C. taiwanensis, 4 -C. metallidurans, 5 -C. pauculus (SAHIN et al, 2000;DE BAERE et al, 2001;CHEN et al, 2001;IWAKI et al, 2008;CUADRADO et al, 2010 Embora apenas a caracterização bioquímica não seja suficiente para identificar espécies bacterianas (CARBONELLE et al, 2011), esta caracterização é bastante útil para que sejam obtidas características fisiológicas dos microrganismos isolados, as quais permitem a otimização de seu cultivo in vitro, levando à obtenção de melhores resultados.…”