Isolation and Characterization of Native Isolates of Bacillus thuringiensis from Vidarbha Region

Padole, Dipika Ashok Rao; Moharil, M. P.; Ingle, Krishnananda Pralhad; Munje, Shyamkant S

doi:10.20546/ijcmas.2017.601.094

Cited by 6 publications

(3 citation statements)

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“…The presence of very highly crystal proteins soluble in high basic pH is aligned with similar findings by Bukhari and Shakoori (2010) while studying the molecular characterization of Bt cry genes and their mosquitocidal activity. The severe pathogenicity levels of Bt parasporal crystal proteins against larvae of S. littoralis and D. melanogaster were explained by the presence of various insecticidal parasporal crystal proteins and by the possibility of different binding sites on the larvae midguts, similar findings reported by Ammouneh et al (2011) and Padole et al (2017). Phylogenetic trees were constructed using MEGA 5 following the multiple alignments of the sequences by Cluster W. The trees were constructed using the neighbor-joining (NJ) method and the maximum parsimony option model, with 1000 bootstrap replicates to estimate the support for each branch Cry-gene specific and competition-binding studies were carried out with lectins, and specific sugars confirmed that Cry1Ac and Cry1Fa share similar numbers of the midgut binding sites of both Anticarsia gemmatalis and Chrysodeixis includes (Bel et al 2017).…”

Section: Discussionsupporting

confidence: 85%

“…The selected colonies were grown on T3 agar medium (per liter: 3 g tryptone, 2 g tryptose, 1.5 g yeast extract, 0.05 M sodium phosphate [pH 6.8], and 0.005 g MnCl (Travers et al 1987)) and incubated at 30°C for 72 h. The cells of Bt potential isolates grown on T3 agar medium were stained with Gram staining as well as spore-staining reagents. All Bt strains were examined for the presence of vegetative cells, spores, and parasporal inclusion (Padole et al 2017). Bt isolates were further characterized by examining the enzymatic activities for catalase, urease, caseinase, gelatinase, amylase, and lecithinase according to Cinar et al (2008).…”

Section: Isolation and Identification Of Native B Thuringiensis Strainsmentioning

confidence: 99%

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Isolation and identification of Bacillus thuringiensis strains native of the Eastern Province of Saudi Arabia

Hassan

Ismail

Elashtokhy

et al. 2021

Egypt J Biol Pest Control

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Background Bacillus thuringiensis (Bt) produces a group of δ-endotoxin proteins designated as cry toxins. No doubt that Bt isolates are excellent potential candidates for biological control strategies. Results The present study showed that 12 Bt strains were isolated and characterized at morphological, biochemical, and molecular levels. All the tested 12 Bt strains were gram-positive, endospore-forming, and possessing typical Bt crystal structures under the scanning electron microscopy (SEM). Universal primers direct and reverse of five pairs were used to detect five Cry-type genes (Cry1, Cry2, Cry3, Cry4, Cry7, and Cry8) by the PCR sizes produced from the studied Bt strains. The 16S rRNA PCR technique, 16S gene primer, DNA template, dNTPs, and Taq polymerase produced unique and distinguishable restriction patterns used for the molecular characterization of the studied Bt strains. Based on the PCR products, the frequency of Cry-gene distribution among the tested strains was Cry1 100%, Cry4 85%, and Cry3 62%, and Cry2 and Cry7 frequency was 54%. Based on the activity of insecticidal of the tested Bt strains, Bt1, Bt9, Bt10, and Bt11 were extremely pathogenic; their pathogenicity ranged from 93 to 100% against dipteran and lepidopteran larvae, compared to the other Bt isolates. The nucleotide sequences of amplified 1500 bp conserved region of 16S rRNA genes of four strains blasted using NCBI database compared to NCBI database sequences, and they were reported as native strains of Bt showing high homology with the known Bt strains (99–100%). The nucleotide sequences of Bt1, Bt9, Bt10, and Bt11 were placed in the GenBank database under accession numbers MN860017, MN843958, MN843959, and MN843960, respectively. Conclusion The strategies of enhancing the sustainability of crops and vegetables that are targeted by a large number of pathogenic insects require a great effort of exploring novel species and strains of Bt. Herein, native strains of Bt were documented from the eastern province of Saudi Arabia that displayed bio-insecticidal action on larvae of Diptera and Lepidoptera.

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Section: Discussionsupporting

confidence: 85%

Section: Isolation and Identification Of Native B Thuringiensis Strainsmentioning

confidence: 99%

Isolation and identification of Bacillus thuringiensis strains native of the Eastern Province of Saudi Arabia

Hassan

Ismail

Elashtokhy

et al. 2021

Egypt J Biol Pest Control

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“…In this result, the different isolates showed variations among one another in their colony color, margin, surface, and elevation. The different colony morphology of Bt was described as white to off-white colony color with irregular colony margin and flat to slightly raised colony elevation (Chai et al 2016 andPadole et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization, and bio-insecticidal efficiency of Ethiopian isolates of Bacillus thuringiensis against Galleria mellonella L. (Lepidoptera: Pyralidae) and tomato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)

Gebremariam

Chekol

Assefa

2021

Egypt J Biol Pest Control

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Background Bacillus thuringiensis is a Gram-positive, rod-shaped, and spore-forming microbial entomopathogen. The silverleaf whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is a worldwide important cryptic species causing serious economic damage to several vegetable crops including tomato. The aim of this study was to characterize and evaluate the bio-insecticidal effects of locally isolated Bacillus thuringiensis (Bt) against Galleria mellonella and Bemisia tabaci. Results Thirty-one isolates of Bt were obtained from 70 soil samples based on cultural characterization coupled with insecticidal crystal protein detection. All of the 31 bacterial isolates were pre-screened for their bio-insecticidal property against the greater wax moth, Galleria mellonella L. larvae. Only 20 (64.5%) isolates were virulent to G. mellonella with a percentage mortality that ranged from 2.50 to 95%. The groEL gene sequences of all 20 entomopathogenic indigenous isolates displayed 99–100% similarity with Bt isolates. Bioassay evaluation of 12 selected isolates against 3rd to 4th nymphal instars of B. tabaci with spore-crystal suspensions of 1 × 109 spores/ml caused 0 to 31.25%, 0 to 57%, and 0 to 82.5% percentage mortality within 48, 96, and 144 h accordingly. Among isolates of Bt, isolate AAUDS-16 had the highest virulence, followed by the isolate AAUES-69D with LC50 values of 9.67 × 106 spores/ml and 1.16 × 107 spores/ml, respectively. The virulent isolates were tested for their growth response to a different temperature range between 15 and 40 °C. All isolates showed a maximum growth rate around 30 °C. Conclusions Bt isolates of AAUES-69D and AAUDS-16 displayed high insecticidal potential against B. tabaci nymphs and G. mellonella larvae, and showed a maximum growth rate in a wide range of temperature. High virulence and temperature-tolerant isolates should be important candidates for diverse insecticidal toxin studies.

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Molecular Characterization of Native Bacillus thuringiensis Strains from Root Nodules with Toxicity Against the Fall Armyworm (FAW, Spodoptera frugiperda) and Brinjal Ash Weevil (Myllocerus subfasciatus)

Delanthabettu

Narasimhappa²,

Asokan

et al. 2022

Curr Microbiol

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Isolation and Characterization of Native Isolates of Bacillus thuringiensis from Vidarbha Region

Cited by 6 publications

References 6 publications

Isolation and identification of Bacillus thuringiensis strains native of the Eastern Province of Saudi Arabia

Isolation and identification of Bacillus thuringiensis strains native of the Eastern Province of Saudi Arabia

Isolation, characterization, and bio-insecticidal efficiency of Ethiopian isolates of Bacillus thuringiensis against Galleria mellonella L. (Lepidoptera: Pyralidae) and tomato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)

Molecular Characterization of Native Bacillus thuringiensis Strains from Root Nodules with Toxicity Against the Fall Armyworm (FAW, Spodoptera frugiperda) and Brinjal Ash Weevil (Myllocerus subfasciatus)

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