Isolation and characterization of microsatellites from<i>Seriola dumerili</i>(Risso 1810)

Porta, José María Peña; Novel, Patricia; Martı́nez-Rodrı́guez, Gonzalo; Álvarez, M. Carmen

doi:10.1111/j.1365-2109.2008.02075.x

Cited by 5 publications

(6 citation statements)

References 8 publications

(6 reference statements)

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“…Amongst these species, only the greater amberjack ( S. dumerili ) is widely distributed in the Mediterranean Sea, being native to the coasts of Spain, France, Italy and Greece (Froese & Pauly ) and, therefore, the preferred species of this genus for aquaculture diversification in these countries. Accordingly, a significant number of studies have been published in relation with the species reproductive biology (Grau ; Marino, Mandich, Massari, Andaloro & Porrello ; Micale, Maricchiolo & Genovese ; García Hernández, García, Zandbergen & Agulleiro ), broodstock management (De la Gándara, Alonso & García‐Gómez ; Roo, Schuchardt, Socorro, Guirao, Hernández‐Cruz & Fernández‐Palacios ), occurrence of spontaneous spawns (Jerez, Samper, Santamaria, Villamandos, Cejas & Felipe ; Jerez, Cejas, Villamandos, Samper, Felipe & Santamaría ), gonad composition (Rodríguez‐Barreto, Jerez, Cejas, Martin, Acosta, Bolaños & Lorenzo ) or microsatellite characterization (Porta, Novel, Martinez‐Rodriguez, Alvarez & Porta ). In addition, several hormonal treatments have been tested in greater amberjack, including human chorionic gonadotropin (HCG) (García, Díaz & Agulleiro ; Kozul, Skaramuca, Glamuzina, Glavic & Tutman ), luteinizing hormone‐releasing hormone analogues (GnRHa) injections (Lazzari, Fusari, Boglione, Marino & Di Francesco ; García‐Gómez & De la Gándara ) and GnRHa implants (Mylonas, Papandroulakis, Smboukis, Papadaki & Divanach ),…”

Section: Introductionmentioning

confidence: 99%

Multiple GnRHa injections to induce successful spawning of wild caught greater amberjack (Seriola dumerili) matured in captivity

et al. 2013

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Reliable reproduction and sufficient amounts of juveniles obtained in captivity constitute a main bottleneck for mass production of greater amberjack. The aim of the present study was to combine several aspects of broodstock management and hormonal induction, to obtain a large number of high quality spawns. Captured Seriola dumerili subadults were kept for three years in 10 m 3 tanks. When fish were larger than 75 cm, they were individually tagged, periodically explored to determine gonad maturation and injected with gonadotropin releasing hormone analogue from June to October. A total of 15 inductions to four males and two females produced 330 821 AE 219 519 eggs/induction and a total of 22 spawns. The number of spawns and female fecundity (2.48 millions eggs/female/spawning season) were similar to those of free wild populations and higher than those previously obtained in captivity for this species. Egg quality increased from an initial low in the first two spawns in June to give, during July to October, a mean of 96.01 AE 6.50% fertilized eggs, 92.58 AE 17.56% hatched eggs, 78.66 AE 12.60% larval survival at day 3 after. These results showed that intramuscular injections of 20 lg kg À1 body weight GnRHa under natural temperature and photoperiod conditions in the Canary Islands produced a high induction efficiency.

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Section: Introductionmentioning

confidence: 99%

Multiple GnRHa injections to induce successful spawning of wild caught greater amberjack (Seriola dumerili) matured in captivity

et al. 2013

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“…Porta et al [13] Argyrosomus regius gCT15, gA2B, CA3, CA4, CA6, CA14, CA13, CA10, gA16, gA17 and gA6…”

Section: Authors Species Locimentioning

confidence: 99%

“…From the microsatellites published in the genetic map of different species of Sciaenidae, 11 microsatellites were markers of meagre, Argyrosomus regius [13], six of Japanese meagre, A. japonicus [10], three of red drum, Sciaenops ocellatus [14,17] and one of yellow meagre, Cynoscion acoupa [11]; therefore, we had 21 pairs of primers which were used to amplify microsatellites using the same PCR conditions (Table 1). The length of the amplicons ranged between 80-266 base pairs (bp), thereby ensuring a wide amplification spectrum, and optimizing the efficiency of the multiplex reaction [27].…”

Section: Primer Desingmentioning

confidence: 99%

“…In the family of the Scianids, many microsatellites have been described [10][11][12][13][14][15][16]. To date, in the case of meagre (Argyrosomus regius), no microsatellite marker panels have been developed, although several specific and interspecific microsatellites have been used in several works [10][11][12][13]17], and only a few studies [18][19][20] showed the multiplex they used for the parental assignment. Vallecillos et al [21] developed a panel of markers similar to this study but with some minor modifications.…”

Section: Introductionmentioning

confidence: 99%

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Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species

Vallecillos

María-Dolores

Villa³

et al. 2022

Fishes

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In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups.

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“…Genetic markers, such as microsatellites, have been successfully applied to a number of aquaculture species to evaluate parentage and pedigree relationships (Gold et al, 2010; Gruenthal & Drawbridge, 2012; Norris et al, 2000; Perez‐Enriquez et al, 2020; Porta et al, 2009; Whatmore et al, 2013). In aquaculture, these analyses are indispensable in developing and evaluating breeding programmes (Fessehaye et al, 2006), minimizing inbreeding effects (Sekino et al, 2003; Vandeputte & Haffray, 2014) and preventing genetic diversity loss due to reproductive variance (Boudry et al, 2002; Rodriguez‐Barreto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Spawning dynamics and egg production characteristics of captive Seriola dorsalis assessed using parentage analyses

Schmidt

Stuart

Hyde

et al. 2021

Aquaculture Research

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In pelagic fish species, studying reproductive behaviour and spawning dynamics is challenging. In culture scenarios, these parameters are difficult to investigate for broadcast spawners such as Seriola. Hubbs‐SeaWorld Research Institute has been culturing California yellowtail (S. dorsalis) since 2003 and now it is poised to become one of the first commercial offshore aquaculture marine finfish species in Southern California. Brood fish are typically kept in large tanks and spawn volitionally throughout the spawning season. This method is successful in yielding a consistent supply of eggs; however, it limits the understanding of spawning group dynamics or individual brood fish contribution, information that will be important to scaling production to commercial levels. In this study, genetic‐based offspring parentage analyses were used to evaluate captive spawning dynamics in S. dorsalis over two consecutive spawning seasons. The analyses determined that typically one female released eggs during a spawning event, compared with an average of six males who released milt during these same spawning events. Notably, the analyses revealed that a single female dominated spawning output in both years, participating in 63 spawning events and producing 49.7 million eggs. The largest single spawn contribution by a female was 2.5 million eggs, corresponding to a batch fecundity estimate of 110,000 eggs kg−1. The spawning interval for the most productive females was 5–6 days. Using genetic tools to characterize spawning events will help to improve breeding programme designs for S. dorsalis, and this information can serve as a proxy for estimating spawning capacities of this species in the wild.

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Isolation and characterization of microsatellites fromSeriola dumerili(Risso 1810)

Cited by 5 publications

References 8 publications

Multiple GnRHa injections to induce successful spawning of wild caught greater amberjack (Seriola dumerili) matured in captivity

Multiple GnRHa injections to induce successful spawning of wild caught greater amberjack (Seriola dumerili) matured in captivity

Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species

Spawning dynamics and egg production characteristics of captive Seriola dorsalis assessed using parentage analyses

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