Isolation and characterization of microsatellite loci in the ascidian <i>Ciona intestinalis</i> (L.)

Procaccini, Gabriele; Pischetola, M.; Lauro, Roberto Di

doi:10.1046/j.1365-294x.2000.01071-4.x

Cited by 11 publications

(11 citation statements)

References 5 publications

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“…31 and 32). In this respect, microsatellites lend themselves as informative markers for phylogeography, migration, and recruitment studies (23,24).…”

Section: Discussionmentioning

confidence: 99%

“…Eighty Ciona spp. individuals (20 from each locality per population) were genotyped with four previously reported (23) and eight newly selected microsatellite markers (24). Allele detection was conducted by using an automated capillary sequencer (CEQ 2000XL DNA Analysis system; Beckman Coulter, Fullerton, CA), and electropherograms were analyzed with Beckman CEQ 2000 version 3.0 software.…”

Section: Methodsmentioning

confidence: 99%

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Cryptic speciation in a model invertebrate chordate

Caputi

Andreakis²,

Mastrototaro³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

178

218

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We applied independent species concepts to clarify the phylogeographic structure of the ascidian Ciona intestinalis, a powerful model system in chordate biology and for comparative genomic studies. Intensive research with this marine invertebrate is based on the assumption that natural populations globally belong to a single species. Therefore, understanding the true taxonomic classification may have implications for experimental design and data management. Phylogenies inferred from mitochondrial and nuclear DNA markers accredit the existence of two cryptic species: C. intestinalis sp. A, genetically homogeneous, distributed in the Mediterranean, northeast Atlantic, and Pacific, and C. intestinalis sp. B, geographically structured and encountered in the North Atlantic. Species-level divergence is further entailed by crossbreeding estimates. C. intestinalis A and B from allopatric populations cross-fertilize, but hybrids remain infertile because of defective gametogenesis. Although anatomy illustrates an overall interspecific similarity lacking in diagnostic features, we provide consistent tools for in-field and in-laboratory species discrimination. Finding of two cryptic taxa in C. intestinalis raises interest in a new tunicate genome as a gateway to studies in speciation and ecological adaptation of chordates.anatomy ͉ Ciona intestinalis ͉ cryptic species ͉ gamete compatibility ͉ phylogenetics

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“…31 and 32). In this respect, microsatellites lend themselves as informative markers for phylogeography, migration, and recruitment studies (23,24).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cryptic speciation in a model invertebrate chordate

Caputi

Andreakis²,

Mastrototaro³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

178

218

View full text Add to dashboard Cite

show abstract

“…Specimens were multilocus genotyped with 4 previously reported [21] and 8 newly selected microsatellite markers [64]. Allele detection was conducted using an automated capillary CEQ 2000XL DNA Analysis system (Beckman Coulter; http://www.beckmancoulter.com) and electropherograms were analyzed with the Beckman CEQ 2000 v3.0 software.…”

Section: Methodsmentioning

confidence: 99%

Natural Variation of Model Mutant Phenotypes in Ciona intestinalis

et al. 2008

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BackgroundThe study of ascidians (Chordata, Tunicata) has made a considerable contribution to our understanding of the origin and evolution of basal chordates. To provide further information to support forward genetics in Ciona intestinalis, we used a combination of natural variation and neutral population genetics as an approach for the systematic identification of new mutations. In addition to the significance of developmental variation for phenotype-driven studies, this approach can encompass important implications in evolutionary and population biology.Methodology/Principal FindingsHere, we report a preliminary survey for naturally occurring mutations in three geographically interconnected populations of C. intestinalis. The influence of historical, geographical and environmental factors on the distribution of abnormal phenotypes was assessed by means of 12 microsatellites. We identified 37 possible mutant loci with stereotyped defects in embryonic development that segregate in a way typical of recessive alleles. Local populations were found to differ in genetic organization and frequency distribution of phenotypic classes.Conclusions/SignificanceNatural genetic polymorphism of C. intestinalis constitutes a valuable source of phenotypes for studying embryonic development in ascidians. Correlating genetic structure and the occurrence of abnormal phenotypes is a crucial focus for understanding the selective forces that shape natural finite populations, and may provide insights of great importance into the evolutionary mechanisms that generate animal diversity.

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“…), while the last three were selected from a DNA library (Procaccini et al . ). Microsatellite PCR reactions followed published protocols with slight modifications.…”

Section: Methodsmentioning

confidence: 97%

High connectivity and directional gene flow in European Atlantic and Mediterranean populations ofCiona intestinalissp. A

et al. 2014

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Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.

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Isolation and characterization of microsatellite loci in the ascidian Ciona intestinalis (L.)

Cited by 11 publications

References 5 publications

Cryptic speciation in a model invertebrate chordate

Cryptic speciation in a model invertebrate chordate

Natural Variation of Model Mutant Phenotypes in Ciona intestinalis

High connectivity and directional gene flow in European Atlantic and Mediterranean populations ofCiona intestinalissp. A

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