Isolation and characterization of microsatellite DNA markers in the grass <i>Poa alpina</i> L.

Maurer, Katrin; Gautschi, B.; Weyand, Anne; Stöcklin, Jürg; Fischer, Markus

doi:10.1111/j.1471-8286.2005.01019.x

Cited by 10 publications

(19 citation statements)

References 10 publications

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“…Clear and distinct microsatellite alleles (i.e., bands), or absence of such bands, were scored manually. The locus-specifi c size range of microsatellite amplicons was similar to the pilot study by Maurer et al (2005) . No PCR dropouts occurred; i.e., all individuals yielded readable banding profi les.…”

Section: Measurements-supporting

confidence: 51%

“…2.5 U polymerase (all latter substances provided by the manufacturer). PCRs were always run in the same machine (Eppendorf Mastercycler gradient, Eppendorf, Hamburg, Germany), with the same cycling conditions as in Maurer et al (2005) . Annealing temperature also followed the latter In the more severe conditions of the Alps with shorter growing seasons, asexual reproduction becomes more important than sexual reproduction and an increase of clonal plant species at higher elevations has therefore been observed ( Billings and Mooney, 1968 ;Bliss, 1971 ;Klimeš et al, 1997 ;Stöcklin et al, 2009 ;Št'astná et al, 2012 ).…”

Section: Molecular Analyses -Sample Collection-mentioning

confidence: 99%

“…Amplifi cation of microsatellite alleles of fi ve loci (CA1D4, GAC1, GA1C3, CA1F4, CAB12) was done as in the pilot study by Maurer et al (2005) . Some modifi cations were applied, i.e., PCR was performed with self-dissolving illustra puReTaq Ready-To-Go PCR Beads (GE Healthcare, Buckinghamshire, UK).…”

Section: Molecular Analyses -Sample Collection-mentioning

confidence: 99%

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Distribution of bulbil‐ and seed‐producing plants of Poa alpina (Poaceae) and their growth and reproduction in common gardens suggest adaptation to different elevations

Steiner

Armbruster

Scheepens

et al. 2012

American J of Botany

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Section: Measurements-supporting

confidence: 51%

Section: Molecular Analyses -Sample Collection-mentioning

confidence: 99%

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Distribution of bulbil‐ and seed‐producing plants of Poa alpina (Poaceae) and their growth and reproduction in common gardens suggest adaptation to different elevations

Steiner

Armbruster

Scheepens

et al. 2012

American J of Botany

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“…To account for potential regional and cultural differences, we had selected four villages of each of the three main cultural traditions in the Swiss Alps, Romanic, Germanic, and Walser . The experiment also served for estimating broad-sense heritability of several vegetative and reproductive characters of P. alpina and to relate it to plant species diversity and microsatellite diversity of Poa alpina known for all sites from previous studies (Maurer et al 2005(Maurer et al , 2006Rudmann-Maurer et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…In the valleys of Romanic regions, more diverse types of land use are still found than in the valleys of the other cultural traditions (Maurer et al 2006). If plants disperse between differently used grassland sites, the higher land use diversity in Romanic regions may suggest that not only microsatellite variation (Maurer et al 2005;RudmannMaurer et al 2007) but also heritable genetic variation of P. alpina is higher in Romanic regions.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of Poa alpina to altitude and land use in the Swiss Alps

et al. 2011

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Current land use and climate change are prompting questions about the ability of plants to adapt to such environmental change. Therefore, we experimentally addressed plant performance and quantitative-genetic diversity of the common Alpine Meadow Grass Poa alpina. We asked how land use and altitude affect the occurrence of P. alpina in the field and whether its common-garden performance suggests adaptation to conditions at plant origin and differences in quantitative genetic diversity among plant origins. Among 216 candidate grassland sites of different land use and altitude from 12 villages in the Swiss Alps, P. alpina occurred preferentially in fertilized and grazed sites and at higher elevations. In a common garden at 1,500 m asl, we grew two plants of [600 genotypes representing 78 grassland sites. After 2 years, nearly 90% of all plants had reproduced. In agreement with adaptive advantages of vegetative reproduction at higher altitudes, only 23% of reproductive plants from lower altitudes reproduced via vegetative bulbils, but 55% of plants from higher altitudes. In agreement with adaptive advantages of reproduction in grazed sites, allocation to reproductive biomass was higher in plants from grazed grasslands than from mown ones. For 53 grasslands, we also investigated broad-sense heritability H 2 , which was significant for all studied traits and twice as high for grazed as for mown grasslands. Moreover, possibly associated with their higher landscape diversity, H 2 was higher for sites of villages of Romanic cultural tradition than for those of Germanic and Walser traditions. We suggest promoting diverse land use regimes to conserve not only landscape and plant species diversity, but also adaptive genetic differentiation and heritable genetic variation.

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Environmental filtering, not local adaptation of established plants, determines the occurrence of seed- and bulbil-producing Poa alpina in a local flora

Stöcklin

Armbruster

2016

Basic and Applied Ecology

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Genetic variability among subspecies, ecotypes and populations is an important component of biodiversity, particularly when habitats are characterized by steep environmental gradients across short distances as in the landscape of the European Alps. In Poa alpina population differentiation is high due to polyploidy, facultative apomixis and the occurrence of seed-and bulbil-producing plants. We analysed microsatellite diversity of seed-and bulbil-producing populations from two nearby alpine grassland sites in the Swiss Alps and transplanted them reciprocally to test the hypothesis that established plants are locally adapted. The seed-and bulbil-producing populations differed molecularly strongly from each other despite their proximity. The seedproducing plants from the rich, calcareous grassland site performed well at both the home and the away site. Conversely, the bulbil-producing plants from the species-poor grassland had a comparatively low productivity at both sites, but performed better at the high-quality away site compared to its home site. Thus, local adaptation of established plants could not be confirmed by reciprocal transplantation. Results suggest that the distribution of seed-and bulbil-producing Poa alpina is a result of habitat quality, differences in plant performance, and the requirements for successful offspring recruitment. The prevailing occurrence of bulbil-producing plants at low productivity sites is probably due to different regeneration niches for seeds vs. bulbils, i.e. an advantage of bulbils at cold sites with poor soil and short growing seasons at this elevation.

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Isolation and characterization of microsatellite DNA markers in the grass Poa alpina L.

Cited by 10 publications

References 10 publications

Distribution of bulbil‐ and seed‐producing plants of Poa alpina (Poaceae) and their growth and reproduction in common gardens suggest adaptation to different elevations

Distribution of bulbil‐ and seed‐producing plants of Poa alpina (Poaceae) and their growth and reproduction in common gardens suggest adaptation to different elevations

Adaptation of Poa alpina to altitude and land use in the Swiss Alps

Environmental filtering, not local adaptation of established plants, determines the occurrence of seed- and bulbil-producing Poa alpina in a local flora

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