Isolation and characterization of microsatellite loci from the mulberry, Morus L.

Development and validation of SSR markers for Coffea arabica L.

“…The efficiency of each method is indicated by the proportion of clones containing SSRs in relation to the total number of clones examined (Zane et al 2002). The efficiency of 38% in this study of C. arabica was high, compared to the previous studies with mulberry, 26% (Zhao et al 2005), Brassica, 18.5% (Cui et al 2008), piqui (Caryocar brasiliense), 14.4% (Collevatti et al 1999), Jute (Corchorus capsularis), 34.5% (Mir et al 2008) and orange tree (Citrus), 25% (Novelli et al 2006). The additional evidence that the methodology was well executed was demonstrated by the fact that all clones presented sequenced inserts and none presented sequenced Escherichia Coli DNA contamination.…”

“…The development of SSR primers using enriched genomic library has been widely used for many species of plants such as eucalyptus (Brondani et al 1998), piqui (Collevatti et al 1999), pepper (Buso et al 2000), sugarcane (Cordeiro et al 2000), bean (Benchimol et al 2007), rice (Brondani et al 2001), avocado (Ashworth et al 2004), lychee (Viruel and Hormaza 2004), melon (Ritschel et al 2004), hop (Stajner et al 2005), mulberry (Zhao et al 2005) and wheat (Song et al 2005). RF Missio et al For Coffea species, a small number of SSR primers have been developed and are available for genetic studies, when compared to cultures such as maize, soybean, rice, wheat and barley (http:// www.gramene.org/).…”

Phylogenetics of Morus (Moraceae) Inferred from ITS and trnL-trnF Sequence Data

“…The ITS and trnL-trnF regions do not provide sufficient variation to resolve relationships at the "tips" of the tree within Morus. Zhao et al (2005) used sequences of ITS and the trnL intron to infer relationships based on genetic distance among some Asian taxa (with additional population sampling relative to the present study), but parsimony analysis of their sequences (obtained from GenBank; data not shown) yields unresolved relationships. Future studies employing more variable DNA regions with extensive sampling (including the breadth of diversity considered here) will be valuable.…”

“…Zhao et al (2005) sequenced the ITS region of nrDNA and the chloroplast trnL intron to investigate genetic distances among some Asian taxa (including M. alba, M. australis, M. macroura, and M. mongolica). Molecular markers widely used in population genetic studies including randomly amplified polymorphic DNA (RAPDs) and inter simple sequence repeats (ISSRs) have also been employed to study patterns of genetic relationships among species of Asian Morus (e.g.…”

Microsatellite Isolation and Characterization in Japanese Persimmon (Diospyros kaki)

“…Microsatellite markers or simple sequence repeats (SSRs) have been used extensively in genetic diversity analysis and DNA genotyping in recent years (Zhao et al 2005;Xie et al 2006). Important advantages such as codominant inheritance, multiple alleles at a single locus, high informativeness, discriminatory power, and reproducibility make them suitable for fingerprinting and analysis of genetic relatedness.…”