Isolation and characterization of microsatellite markers for Cedrela odorata L. (Meliaceae), a high value neotropical tree

Hernández, Gustavo J. Acevedo; Buonamici, A.; Walker, Kim; Vendramin, G. G.; Navarro, Carlos; Cavers, Stephen

doi:10.1007/s10592-007-9334-y

Cited by 16 publications

(11 citation statements)

References 9 publications

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“…When compared to other congeners, C. fissilis harbors unusually high levels of genetic diversity. The mean value of expected heterozygosity ( H E = 0.82) was larger in C. fissilis than in the congeners C. balansae (mean H E = 0.62; Soldati et al, 2013) and C. lilloi (mean H E = 0.09; Inza et al, 2012) from the Argentinean Yungas; nevertheless, C. odorata from Costa Rica displayed a value of expected heterozygosity (mean H E = 0.87; Hernandez et al, 2008) that was very similar to what we observed for C. fissilis . It is usual to find high levels of genetic diversity in congeners that harbor wind pollination systems, long generation times, cross‐breeding and fertility systems, and occur in habitats that represent older stages of succession (Hamrick et al, 1979).…”

Section: Discussionmentioning

confidence: 88%

“…Genotyping was carried out using 12 microsatellite loci: eight markers (Ced2, Ced18, Ced41, Ced44, Ced54, Ced65, Ced95, and Ced131) developed by Hernandez et al (2008) for C. odorata , and three markers (CF26, CF34, and CF66) developed by Gandara (2009) for C. fissilis (Table 2). Primer pair CF66 amplified two loci—CF66A and CF66B, respectively (Gandara, 2009); distinct ranges of allele sizes (113–175 bp for CF66A; 199–253 bp for CF66B) allowed us to clearly differentiate the genotypes of each of the two loci (Table 2).…”

Section: Methodsmentioning

confidence: 99%

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Contemporary patterns of genetic diversity of cedrela fissilis offer insight into the shaping of seasonal forests in eastern South America

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Vinson

Rody

et al. 2016

American J of Botany

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In C. fissilis, genetic diversity is structured according to geography: the Atlantic range and the Chiquitano range each harbor a genealogical lineage. Interfertility and varying levels of admixture between lineages provide strong evidence that the lineages evolved under geographic, but not genetic, isolation. Admixture is of recent origin, owing to population expansion. Cedrela fissilis shares this dual pattern of distribution of genetic diversity with other phylogenetically unrelated taxa that are typically associated with seasonal forests.

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Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Contemporary patterns of genetic diversity of cedrela fissilis offer insight into the shaping of seasonal forests in eastern South America

Mangaravite

Vinson

Rody

et al. 2016

American J of Botany

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“…Total genomic DNA was extracted according to Cota-Sánchez et al (2006) with modifications (Riahi et al, 2010). Genotyping was carried out using 11 microsatellite loci: 8 markers (Ced2, Ced18, Ced41, Ced44, Ced54, Ced65, Ced95, and Ced1; Table 2) developed by Hernández et al (2008) for Cedrela odorata, and three markers (CF26 and CF66) developed by Gandara et al (2014) for C. fissilis (Table 2). Primer pair CF66 amplified two loci-CF66A and CF66B, respectively (Gandara et al, 2014); distinct ranges of allele sizes (113-167 bp for CF66A; 199-253 bp for CF66B) allowed us to clearly differentiate the genotypes of each of the two loci (Table 2).…”

Section: Dna Extraction Microsatellite Genotyping and Gene Sequencingmentioning

confidence: 99%

Genetic diversity of Cedrela fissilis (Meliaceae) in the Brazilian Atlantic Forest reveals a complex phylogeographic history driven by Quaternary climatic fluctuations

Mangaravite

Silveira

Huamán-Mera

et al. 2019

J of Sytematics Evolution

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Quaternary climatic fluctuations have shaped the geographic distribution of lineages, potentially affecting the demography, genetic structure, and patterns of genetic diversity of extant species. Different phylogeographic scenarios have been proposed for plants in neotropical cloud forests during the Last Glacial Maximum based on paleoecological data: the dry refugia hypothesis (DRH) and the moist forest hypothesis. We specifically focus on the Brazilian Atlantic Forest (BAF) range of Cedrela fissilis (Meliaceae), sampling 410 specimens from 50 localities. Our study combines analyses of the genetic diversity, phylogeographic patterns, and past geographic distributions with a particular focus on highland populations. We identified 283 alleles across the 11 microsatellite loci, ranging from 18 to 33 alleles per locus, distributed across five genetic groups. Most populations of C. fissilis from the BAF exhibited a diffuse genetic structure, reflected in low pairwise FST values, which could be the consequence of high gene flow. In addition, the plastid data showed a connection between the western, southern, and eastern populations in the North‐East of Brazil, but no association between genetic data and elevation was observed. Habitat suitability projections over the past 140 000 years showed less fragmentation relative to the present, indicating a higher connectivity and gene flow. Our results provide support for both the moist forest as well as the DRH, suggesting that most likely, a mixture of these processes has acted through space and time.

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“…La amplificación de ADN se llevó a cabo utilizando nueve primers ya existentes para C. odorata (Hernández et al 2008) y uno más diseñado con el programa Primer 3 y comprobado con el programa Pick Primers a partir de secuencias almacenadas en el banco de genes del Centro Nacional de Información de Biotecnología (NCBI) 1 (cuadro 2). La mezcla de reacción consistió de 3,5 μL de H 2 O, 12,5 μL de Go Taq® Hot Start Green Master Mix 1X, 1 μL de "upstream primer" (10 μM), 1 μL de "downstream primer" (10 μM) y 2 μL de ADN template.…”

Section: Métodosunclassified

Diversidad genética del cedro rojo ( Cedrela odorata ) en el estado de Tabasco, México

et al. 2018

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The red cedar (Cedrela odorata) is a valuable timber species. It is currently listed as a protected species, as well as on the IUCN red list due to the decline of its wild populations mainly due to habitat fragmentation and deforestation. The aim of the present study was to estimate the structure and genetic diversity of red cedar populations in the state of Tabasco, Mexico. Sixty eight samples of C. odorata from the states of Tabasco and Chiapas were analyzed. The DNA was extracted with the CTAB method (cetyl trimethyl ammonium bromide). DNA amplification was performed using 10 microsatellite loci (SSRs), nine of them previously reported as polymorphic for the species and one designed (AB0507) with the program Primer 3. For the analysis of the resulting data, NTsys, GenAlex and Cervus pc programs were used. Genetic differentiation was high (Fst = 0.419) and genetic diversity was low (Ho = 0.297). Compared with other studies, it showed less genetic diversity of the species, indicating superior genetic erosion, presently. With this study, it is possible to suggest more suitable populations for the extraction of germplasm and, thus, counteract the loss of genetic diversity of this species in the region.

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Isolation and characterization of microsatellite markers for Cedrela odorata L. (Meliaceae), a high value neotropical tree

Cited by 16 publications

References 9 publications

Contemporary patterns of genetic diversity of cedrela fissilis offer insight into the shaping of seasonal forests in eastern South America

Contemporary patterns of genetic diversity of cedrela fissilis offer insight into the shaping of seasonal forests in eastern South America

Genetic diversity of Cedrela fissilis (Meliaceae) in the Brazilian Atlantic Forest reveals a complex phylogeographic history driven by Quaternary climatic fluctuations

Diversidad genética del cedro rojo ( Cedrela odorata ) en el estado de Tabasco, México

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