Isolation and characterization of microsatellite loci for the cavehopper Palmorchestia hypogaea (Amphipoda: Talitridae)

Villacorta, Carlos Darwin Angulo; Cánovas, Fernando; Oromı́, Pedro; Juan, Carlos

doi:10.1007/s12686-009-9093-9

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…A few loci showed significant deviations from Hardy-Weinberg equilibrium after Bonferroni correction (probably due to the presence of null alleles). Average global F ST values not using and using ENA method were 0.13 and 0.12, respectively, and the corrected global F ST values per locus varied from 0.05 to 0.24, also being always very similar to the uncorrected ones (results not shown), suggesting in our case that even when present, null alleles have very little impact on the F ST estimates and should not lead to misleading interpretations (Chapuis et al 2005;Chapuis and Estoup 2007;Meglecz et al 2004;Villacorta et al 2009). No scoring errors and no evidence of linkage disequilibrium were detected between any pairs of loci after Bonferroni correction.…”

Section: Microsatellitessupporting

confidence: 59%

Mitochondrial DNA and microsatellites reveal significant divergence in the beachflea Orchestia montagui (Talitridae: Amphipoda)

et al. 2012

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Talitrids are semiterrestrial crustacean amphipods inhabiting sandy and rocky beaches; they generally show limited active dispersal over long distances. In this study we assessed levels of population genetic structure and variability in the talitrid amphipod Orchestia montagui, a species strictly associated to stranded decaying heaps of the seagrass Posidonia oceanica. The study is based on six populations (153 individuals) and covers five basins of the Mediterranean Sea (Tyrrhenian, Ionian, Adriatic, Western and Eastern basins). Samples were screened for polymorphisms at a fragment of the mitochondrial DNA (mtDNA) coding for the cytochrome oxidase subunit I gene (COI; 571 base pairs) and at eight microsatellite loci. MtDNA revealed a relatively homogeneous haplogroup, which clustered together the populations from the Western, Tyrrhenian and Eastern basins, but not the populations from the Adriatic and Ionian ones; microsatellites detected two clusters, one including the Adriatic and Ionian populations, the second grouping all the others. We found a weak geographic pattern in the genetic structuring of the species, with a lack of isolation by distance at either class of markers. Results are discussed in terms of probability of passive dispersal over long distances through heaps of seagrass

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Section: Microsatellitessupporting

confidence: 59%

Mitochondrial DNA and microsatellites reveal significant divergence in the beachflea Orchestia montagui (Talitridae: Amphipoda)

et al. 2012

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“…() used RAPDs to explore intra‐ and interspecific genetic differentiation in two species of caprellidean amphipods. Microsatellites were developed for numerous amphipods (Baird, Miller, & Stark, ; Rewicz, Wattier, Rigaud, Bacela‐Spychalska, & Grabowski, ; Villacorta, Canovas, Oromi, & Juan, ; Weiss & Leese, ), but were never used for taxonomic purposes and are not considered here.…”

Section: Taxonomic Practices In Delimiting Cryptic Speciesmentioning

confidence: 99%

Cryptic species as a window into the paradigm shift of the species concept

2018

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The species concept is the cornerstone of biodiversity science, and any paradigm shift in the delimitation of species affects many research fields. Many biologists now are embracing a new "species" paradigm as separately evolving populations using different delimitation criteria. Individual criteria can emerge during different periods of speciation; some may never evolve. As such, a paradigm shift in the species concept relates to this inherent heterogeneity in the speciation process and species category-which is fundamentally overlooked in biodiversity research. Cryptic species fall within this paradigm shift: they are continuously being reported from diverse animal phyla but are poorly considered in current tests of ecological and evolutionary theory. The aim of this review is to integrate cryptic species in biodiversity science. In the first section, we address that the absence of morphological diversification is an evolutionary phenomenon, a "process" counterpart to the long-studied mechanisms of morphological diversification. In the next section regarding taxonomy, we show that molecular delimitation of cryptic species is heavily biased towards distance-based methods. We also stress the importance of formally naming of cryptic species for better integration into research fields that use species as units of analysis. Finally, we show that incorporating cryptic species leads to novel insights regarding biodiversity patterns and processes, including large-scale biodiversity assessments, geographic variation in species distribution and species coexistence. It is time for incorporating multicriteria species approaches aiming to understand speciation across space and taxa, thus allowing integration into biodiversity conservation while accommodating for species uncertainty.

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“…Gene flow is limited to exchanges between nearby surface populations of P. epigea. Villacorta et al (2009) and Pavesi et al (in Bonizzoni et al, 2011) described the isolation of microsatellite loci (eight each) for P. hypogaea and O. montagui, respectively. No microsatellite data are available as yet for P. hypogea to complement the existing mtDNA data.…”

Section: Main Findingsmentioning

confidence: 99%

Patterns of genetic structuring and levels of differentiation in supralittoral talitrid amphipods: an overview

Pavesi

Ketmaier

2013

Crustac

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Talitrids are the only family within the order Amphipoda to have colonised supralittoral and terrestrial environments. They live in a variety of settings, from sandy to rocky and pebble beaches, to river and lake banks, and to leaf litter and caves. A cotnmon feature is the absence of a planktonic larval stage to facilitate passive dispersal over long-distances. However, some species have broad distributions. Genetic studies over the past 25 years have tried to explain this apparent contradiction by assessing patterns of species genetic structuring on different geographical scales. Here, we review the molecular studies available to date and focus on the population genetics of talitrids. Most of these studies considered populations in the Mediterranean area, but also along the Atlantic coast and in Canary Island caves. From this review, the group emerges as a potential model to understand processes of dispersal and divergence in non-highly-vagile supralittoral organisms. At the same time, studies on these issues are still too restricted geographically: a worldwide scale including different regions would provide us with a better perspective on these problems. RIASSUNTOLa famiglia Talitridae è Tunica, all'intemo dell'ordine Amphipoda, ad essere rappresentata da specie che hanno colonizzato l'ambiente emerso. Queste si possono trovare in una grande varietà di habitat: dalle spiagge sabbiose, rocciose e ciottolose, alie rive dei fiumi e dei laghi, fino alie lettiere delle foreste umide o agli ambienti cavernicoli. Una caratteristica comune a tutti gli anfipodi è l'assenza di uno stadio larvale planctónico che faciliti o promuova una dispersione passiva su distanze medio-lunghe. Nonostante questo, alcune specie presentano un areale piuttosto esteso. GU studi genetici condotti negU ultimi 25 anni hanno cercato di spiegare questa apparente contraddizione attraverso stime ed analisi della struttura genética di specie diverse su scale geografiche piti o menô ) GENETIC STRUCTURING IN SUPRALITTORAL TALITRIDS 891 estése. In questo lavoro ci proponiamo quindi di riassumere gh studi molecolari condotti sino ad oggi ed aventi come oggetto la genética di popolazione degli anfipodi talitridi. La maggior parte dei lavori a disposizione in letteratura considera popolazioni e specie dell'area Mediterránea, ma alcuni coprono anche le coste atlantiche nord-orientali e le grotte delle isole Canarie. Da questi studi emerge chiaramente la grande potenzialità dei talitridi come gruppo-modello ideale per comprendere i processi di dispersione e di divergenza in organismi caratterizzati da bassi livelli di vagilita. Alio stesso tempo, le conclusioni fin qui ottenute si riferiscono ad aree geografiche relativamente hmitate: da qui la nécessita di amphare la scala spaziale. Questi lavori rappresenterebbero un contributo sostanziale, consentendo, da un lato, di migliorare la nostra conoscenza dei modelli di strutturazione genética di questo gruppo di crostacei, e dall'altro di meglio comprendere le dinamiche evolutive che regolano le comunità de...

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Isolation and characterization of microsatellite loci for the cavehopper Palmorchestia hypogaea (Amphipoda: Talitridae)

Cited by 3 publications

References 11 publications

Mitochondrial DNA and microsatellites reveal significant divergence in the beachflea Orchestia montagui (Talitridae: Amphipoda)

Mitochondrial DNA and microsatellites reveal significant divergence in the beachflea Orchestia montagui (Talitridae: Amphipoda)

Cryptic species as a window into the paradigm shift of the species concept

Patterns of genetic structuring and levels of differentiation in supralittoral talitrid amphipods: an overview

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