Isolation and characterization of microsatellite markers in the new world screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)

Abstract

The new world screw‐worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw‐worm, a set of 10 polymorphic microsatellite markers was isolated from an AC‐enriched genomic library. Amplification of the reported loci in 30 screw‐worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross‐species amplifications suggested that these loci may be useful in other Calliphoridae species.

Microsatellite markers for population genetic studies of the blowfly Chrysomya putoria (Diptera: Calliphoridae)

“…average was slightly smaller than the average number of alleles observed in C. albiceps microsatellites, 5.7 (Torres & Azeredo-Espin 2008), but both were substantially smaller than the average allele number per locus observed in the native South American Calliphoridae, Chrysomya hominivorax, 7.4 (Torres et al 2004, Torres & Azeredo-Espin 2005. The reduced variability at Chrysomya microsatellite loci is in agreement with the expectation that introduced populations show reduced levels of diversity because of founder effects and post-introduction demographic bottlenecks (Dlugosch & Parker 2008).…”

Development of new polymorphic microsatellite markers for the New World screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)

“…Although an (AG) 13 oligomer was used for the precloning enrichment, other repeat motifs were also found in the cloned products. No redundant loci were detected when compared with those described by Torres et al . (2004).…”

“…Ten dinucleotide microsatellites for the NWS have already been developed (Torres et al . 2004), but more are needed to increase the number of markers available for building polymerase chain reaction (PCR) multiplexed systems and to reduce the laboratory costs in automated genetic analysis.…”

“…Microsatellites were isolated using a different protocol from that described in Torres et al . (2004) and the repeat probe (AG) n was used instead of (GT) n in the enrichment step.…”

Genetic Approaches for Studying Myiasis-causing Flies: Molecular Markers and Mitochondrial Genomics

“…These contemporary markers range from the analysis of mtDNA by restriction fragment length polymorphism (RFLP) (Roehrdanz, 1989;Roehrdanz and Johnson, 1988;Infante & AzeredoEspin, 1995;Geurgas, Infante-Malachias and Azeredo-Espin, 2000), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (Litjens, Lessinger & AzeredoEspin, 2001) and sequencing of the mitochondrial genome of C. hominivorax , C. chloropyga (=C. putoria) and D. hominis (Azeredo-Espin et al, accession number AY 463155) to highly polymorphic markers such as the mtDNA-Control Region Junqueira et al, 2002), random amplified polymorphic DNA (RAPD), and microsatellite DNAs (Azeredo-Espin, 1993; Infante and Azeredo-Espin, 1995; Infante-Malachias, Yotoko and Azerodo-Espin, 1999;AzeredoEspin, 2001;Skoda, Pornkulwat Foster, 2002;Torres et al, 2004).…”