Isolation and characterization of mesenchymal stem cells derived from fetal bovine liver

Lu, Tianjian; Hu, Pengfei; Su, Xiaohua; Li, Changli; Ma, Yuehui; Guan, Weijun

doi:10.1007/s10561-013-9410-0

Cited by 21 publications

(19 citation statements)

References 31 publications

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“…In-depth analysis has been performed for BMSCs (4,5), which exist in a wide range of tissues (6)(7)(8). Many researchers (6,8,9) believe that MSCs originate from adult stem cells.…”

Section: Introductionmentioning

confidence: 99%

Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

Cai

Yang

et al. 2016

Oncology Reports

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In recent years, mesenchymal stem cells (MSCs), which possess the ability to specifically home to tumor sites, with the potential of multi-directional differentiation and low immunogenicity, have been reported to inhibit the growth of various types of tumors. In the present study, we isolated MSCs from the rib perichondrium (PMSCs). By comparing PMSCs with bone marrow‑derived mesenchymal stem cells (BMSCs), we demonstrated that PMSCs present biological characteristics similar to those of BMSCs. Furthermore, we explored the effect and antitumor mechanism of PMSCs in rat SHZ-88 breast cancer cells. The growth, migration and invasion of the SHZ-88 cells were significantly inhibited, and the Wnt/β-catenin pathway and its target genes were downregulated in the SHZ-88 cells by PMSC-conditioned medium. The expression level of dickkopf-1 (DKK-1) was higher in the PMSCs than that noted in the SHZ-88 cells. Neutralization of DKK-1 in the PMSC‑conditioned medium attenuated the inhibitory effects of PMSCs on SHZ-88 cells. Therefore, PMSC-secreted DKK-1 is involved in the inhibition of SHZ-88 cell growth, migration and invasion, via the Wnt/β‑catenin signaling pathway. In addition, we demonstrated that PMSCs inhibited the growth of breast cancer in vivo and prolonged the survival time of tumor‑bearing rats. PMSCs inhibited the growth of transplanted breast tumors through the Wnt/β-catenin signaling pathway. In conclusion, our data confirmed that MSCs derived from the perichondrium present biological characteristics similar to those of BMSCs and inhibit the growth of breast cancer cells through the Wnt/β-catenin signaling pathway in vitro and in vivo. DKK-1 secreted by PMSCs played a vital role in controlling the Wnt/β-catenin signaling pathway in breast cancer.

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“…In-depth analysis has been performed for BMSCs (4,5), which exist in a wide range of tissues (6)(7)(8). Many researchers (6,8,9) believe that MSCs originate from adult stem cells.…”

Section: Introductionmentioning

confidence: 99%

Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

Cai

Yang

et al. 2016

Oncology Reports

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“…However, all encompass characteristics of multilineage differentiation potential [24,25]. Lu et al [8] have reported the isolation and characterization of MSCs that were derived from a fetal bovine liver (LMSCs) in their study. These bovine LMSCs could be subcultured for up to 44 passages, and expressed CD29, CD44, CD73, CD90, CD106, and CD166 but not CD34, CD45, and BLA-DR. Further, bovine LMSCs exhibited substantial proliferating ability and mesodermal differentiation potential.…”

Section: Discussionmentioning

confidence: 99%

“…Many researchers have sought to establish bovine MSCs from various tissues. To date, bovine MSCs have been isolated from bone marrow [6], umbilical cord blood [7], amniotic fluid [1], liver [8], adipose tissue [9], endometrial tissue [10], and Wharton's jelly [11]. The objective of the present study was to isolate MSCs from bovine tongue tissues, and to fully characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) by analyzing cell growth curves, cell surface markers, and stemness such as the mesodermal differentiation potential of Korean native cattle.…”

Section: Introductionmentioning

confidence: 99%

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Lee

Byeon

et al. 2020

Bioscience Reports

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Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In the present study, we attempted to isolate and characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was maintained for up to 20 or more passages. We observed a significant increase in the proliferation of boT-MSCs during the 20 consecutive passages under low-glucose Dulbecco’s modified Eagle’s medium culture condition among the three culture conditions. These boT-MSCs presented pluripotency markers (octamer-binding transcription factor 3/4 (Oct3/4) and sex determining region Y-box2 (Sox2)) and cell surface markers, which included CD13, CD29, CD44, CD73, CD90, CD105, CD166, and major histocompatibility complex (MHC) class I (MHC-I) but not CD11b, CD14, CD31, CD34, CD45, CD80, CD86, CD106, CD117, and MHC-II at third passage. Moreover, these boT-MSCs could differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Thus, the present study suggests that the tongue of bovines could be used as a source of bovine MSCs.

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“…MSCs may be isolated from various sources, including umbilical cord (UC), bone marrow (BM), liver, adipose tissue, multiple dental tissues and induced pluripotent stem cells (28,29). MSCs express CD44, CD73, CD90 and CD105, but not CD45, CD34 and CD14; these cells are characterized by their ability to adhere to plastics under standard cell culture conditions (30).…”

Section: Stem Cellsmentioning

confidence: 99%

Role of stem cell-derived exosomes in cancer

Fei

et al. 2017

Oncology Letters

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Exosomes are small, extracellular membrane- enclosed vesicles that contain a variety of molecules, including proteins, DNA, mRNA and non-coding RNA; these vesicles have been defined as new tools for intercellular communication between cells. Numerous types of cells, including stem cells, secrete exosomes into the extracellular environment, and are significant communicators in the tumor microenvironment. Stem cells are a unique cell population defined by their ability to indefinitely self-renew, differentiate into a variety of cell lines, and form clonal cell populations. Stem cells also secrete large amounts of exosomes, which have demonstrated great potential in a variety of diseases. Increasing evidence has revealed that the mechanism of interaction between stem cells and human tumor cells involves the exchange of biological material through exosomes. In this review, the latest developments in the role of stem cell-derived exosomes in cancer are highlighted.

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Isolation and characterization of mesenchymal stem cells derived from fetal bovine liver

Cited by 21 publications

References 31 publications

Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Role of stem cell-derived exosomes in cancer

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