Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta

Igura, Koichi; Zhang, X.; Takahashi, Kenji; Mitsuru, Ayako; Yamaguchi, Satoru; Takahashi, Tsuneo

doi:10.1080/14653240410005366-1

Cited by 269 publications

(143 citation statements)

References 46 publications

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“…Placental sections of about 10 g (2.5 cm cubes) were extensively minced and then centrifuged at 550 g for 5 min. The pellet was then haemolysed in red blood cell lysis buffer (155 mM NH 4 Cl, 10 mM KHCO 3, 0.1 mM EDTA, pH 8.0), centrifuged at 550 g for 5 min at room temperature. The pellet was incubated at 37 C in 0.05% Trypsin-EDTA solution (Invitrogen/Gibco, California, USA) for 10 minutes, resuspended twice in 50% a-MEM medium/ 50% FBS, subjected to centrifugation at 550 g for 10 min at room temperature and then plated in a-MEM medium supplemented with 20% FCS, 0.02% L-ascorbate-2-phosphate, 1% L-Glutamine, 0.5% penicillin/streptomycin, 0.5% Fungizone and 1% Tylosin.…”

Section: Isolation Of Stem Cells From Term Placental Tissuementioning

confidence: 99%

“…PMSCs selectively attach to the plastic cultureware, proliferate rapidly and are usually prepared without additional enrichment strategies [2]. PMSCs can be differentiated in vitro under specific stimulatory environments into derivatives of the mesenchymal cell lineage such as osteocytes, adipocytes, myocytes and chondrocytes [1,[3][4][5][6][7]. In addition, there is evidence of differentiation in vitro into cell types characteristic of other lineages such as hepatocyte-like cells and neural-like cells [3,4,[7][8][9][10][11], but in vivo evidence for such differentiation is very limited.…”

Section: Introductionmentioning

confidence: 99%

“…FACS analysis has been used routinely for the analysis of PMSC populations [1,[3][4][5][6][7]. In this study, we used cell surface markers, including STRO-1, 3G5, CD146 and CD49a that enrich CFU-F populations, to characterise PMSC preparations by immunohistochemistry.…”

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche

et al. 2010

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Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche Castrechini, N. M.; Murthi, P.; Gude, N. M.; Erwich, Jan Jaap H. M.; Gronthos, S.; Zannettino, A.; Brennecke, S. R.; Kalionis, B.; Brennecke, S.P. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. t r a c tThe chorionic villi of human term placentae are a rich source of mesenchymal stem cells (PMSCs). The stem cell ''niche'' within the chorionic villi regulates how PMSCs participate in placental tissue generation, maintenance and repair, but the anatomic location of the niche has not been defined. A number of cell surface markers for phenotypic characterisation of mesenchymal stem cells (MSCs) were employed to identify the stem cell niche within the chorionic villi of first trimester and term human placenta. This included antibodies to pericyte cell surface markers STRO-1 and 3G5, which have been used to identify mesenchymal stem cells in other tissues, but have not been studied in placental tissues. PMSCs were isolated from term human placentae and shown to have stem cell properties by their ability to grow on untreated plastic culture ware, capacity for forming clones (i.e. clonogenicity) and their capability to differentiate into adipocytes, chondrocytes and osteocytes. Western analysis confirmed that STRO-1 and 3G5 are present in placental protein extracts and in PMSCs. Immunocytochemistry revealed PMSCs were positive for MSC cell surface markers (STRO-1, 3G5, CD105, CD106, CD146, CD49a, a-SMA) and negative for haematopoietic stem cell markers (CD117, CD34) and endothelial markers (CD34, vWF). Immunohistochemistry with antibodies to MSC cell surface markers on first trimester and term tissues revealed a vascular niche for PMSCs. Dual-label immunofluorescence analysis was used to compare STRO-1 antibody staining with that of endothelial cell marker vWF and found no significant overlap in staining. This indicated that some PMSCs have a pericyte-like phenotype. We propose that the vascular niche harbours a pool of PMSCs that can give rise to committed progenitors for tissue maintenance and repair, and that PMSCs contribute to vessel maturation and stabilization.

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Section: Isolation Of Stem Cells From Term Placental Tissuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche

et al. 2010

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“…Cells may also be isolated during an ongoing pregnancy using relatively minimally invasive techniques such as chorionic villus sampling (CVS). It was shown that PMSCs are able to differentiate into adipocytes, osteoblasts, myofibroblasts, hepatic cells and neural-like cells under appropriate conditions [67,70]. The potential of PMSCs to differentiate into different cell types, pretested in experiments in vitro, is then confirmed in many experiments in vivo.…”

Section: Placenta/chorionic Villous Mesenchymal Stem/stromal Cellsmentioning

confidence: 78%

Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion

Witkowska-Zimny

Wróbel

2011

Cellular and Molecular Biology Letters

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Recently, stem cell biology has become an interesting topic, especially in the context of treating diseases and injuries using transplantation therapy. Several varieties of human stem cells have been isolated and identified in vivo and in vitro. Ideally, stem cells for regenerative medical application should be found in abundant quantities, harvestable in a minimally invasive procedure, then safely and effectively transplanted to either an autologous or allogenic host. The two main groups of stem cells, embryonic stem cells and adult stem cells, have been expanded to include perinatal stem cells. Mesenchymal stem cells from perinatal tissue may be particularly useful in the clinic for autologous transplantation for fetuses and newborns, and after banking in later stages of life, as well as for in utero transplantation in case of genetic disorders.This review highlights the characteristics and therapeutic potential of three human mesenchymal stem cell types obtained from perinatal sources: Wharton’s jelly, the amnion, and the chorion.

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“…The large number of sources of MSC-like cells in the adult body is one of the reasons why these cells are so attractive. MSC-like cells have been identified in the dental pulp, 8 skin, 9 umbilical cord blood, 10 placenta, 11 liver and lungs. 5 Under certain in vitro conditions MSCs are capable of differentiating into osteoblasts, adipose cells, cartilage cells, skeletal muscle cells and even into neuron-like cells.…”

Section: Introductionmentioning

confidence: 99%

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

Kuznetsova

Prodanets

Rodimova

et al. 2016

Cell Adhesion & Migration

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Mesenchymal stem cells (MSCs) are thought to be the most attractive type of cells for bone repair. However, much still remains unknown about MSCs and needs to be clarified before this treatment can be widely applied in the clinical practice. The purpose of this study was to establish the involvement of allogeneic MSCs in the bone formation in vivo, using a model of transgenic mice and genetically labeled cells. Polylactide scaffolds with hydroxyapatite obtained by surface selective laser sintering were used. The scaffolds were sterilized and individually seeded with MSCs from the bone marrow of 5-week-old GFP(C) transgenic C57/Bl6 or GFP(¡)C57/Bl6 mice. 4-mm-diameter critical-size defects were created on the calvarial bone of mice using a dental bur. Immediately after the generation of the cranial bone defects, the scaffolds with or without seeded cells were implanted into the injury sites. The cranial bones were harvested at either 6 or 12 weeks after the implantation. GFP(C) transgenic mice having scaffolds with unlabeled MSCs were used for the observation of the host cell migration into the scaffold. GFP(¡) mice having scaffolds with GFP(C) MSCs were used to assess the functioning of the seeded MSCs. The obtained data demonstrated that allogeneic MSCs were found on the scaffolds 6 and 12 weeks post-implantation. By week 12, a newly formed bone tissue from the seeded cells was observed, without an osteogenic predifferentiation. The host cells did not appear, and the control scaffolds without seeded cells remained empty. Besides, a possibility of vessel formation from seeded MSCs was shown, without a preliminary cell cultivation under controlled conditions.

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Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta

Cited by 269 publications

References 46 publications

Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche

Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche

Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

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