Isolation and Characterization of Melanin from a Marine Fungus

Ravishankar, J.P.; Muruganandam, V.; Suryanarayanan, T. S.

doi:10.1515/botm.1995.38.1-6.413

Cited by 43 publications

(41 citation statements)

References 14 publications

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“…Daniel (1938) has showed that the log of optical density of a melanin solution when plotted against wavelength produces a linear curve with negative slopes (Ellis and Griffiths 1974;Suryanarayanan et al 2004). Such characteristic straight lines with negative slopes have been obtained for some terrestrial and marine fungi (Bartnicki-Garcia andReyes 1964, Chet et al 1967;Ellis and Griffiths 1974;Ravishankar et al 1995). The pigment of P. cystidiosus also gave a straight line with a negative slope indicating it as melanin (Fig.…”

Section: Treatment With Melanin Synthesis Inhibitorsmentioning

confidence: 89%

“…The pigment obtained from the fungus was dissolved in 3 ml of Borate buffer (pH 8.0) and its UV spectrum was recorded with Beckman Du-40 spectrophotometer (Ravishankar et al 1995). For IR spectrum, the pigment was further purified by acid hydrolysis with 5 ml of 7 N HCl in a sealed glass vial and kept for 2 h at 100°C (Bell and Wheeler 1986).…”

Section: Spectroscopic Studiesmentioning

confidence: 99%

“…The purified pigment was ground with infrared grade KBr (1: 10) and pressed into disks under vacuum using a Spectra Lab Pelletiser. The spectrum (4,000-400 cm -1 ) was recorded in a Burker 72 FTIR spectrophotometer (Ellis and Griffiths 1974;Ravishankar et al 1995). The EPR spectrum of the pigment was also taken in a Varian E112 EPR Spectrophotometer.…”

Section: Spectroscopic Studiesmentioning

confidence: 99%

“…The decrease in the absorption with increasing wavelength is almost linear in the case of melanins. Hence, the slopes of linear plots are often used to identify melanins (Chet et al 1967;Ellis and Griffiths 1974;Ravishankar et al 1995). Daniel (1938) has showed that the log of optical density of a melanin solution when plotted against wavelength produces a linear curve with negative slopes (Ellis and Griffiths 1974;Suryanarayanan et al 2004).…”

Section: Treatment With Melanin Synthesis Inhibitorsmentioning

confidence: 99%

“…Fungal melanins are conferring certain advantages to fungi such as increasing their survival potential in some environments and enhancing their virulence (Henson et al 1999;Rizner and Wheeler 2003). Although melanin pigments of plant pathogenic fungi (Howard and Ferrari 1989), human pathogenic fungi (Rizner and Wheeler 2003) and saprophytic fungi (Ravishankar et al 1995) have been investigated, the literature pertaining to the pigment in edible mushrooms are meager.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of melanin pigment from Pleurotus cystidiosus (telomorph of Antromycopsis macrocarpa)

Selvakumar

Rajasekar

Periasamy

et al. 2008

World J Microbiol Biotechnol

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Melanins are enigmatic pigments that are produced by a wide variety of microorganisms including several species of bacteria and fungi. For more than 40 years, fungi have been known to produce pigments called melanins. Melanin pigment production by mushrooms was not intensively studied. The present study was carried out on isolation and characterization of melanin from an edible mushroom Pleurotus cystidiosus var. formosensis. The mushroom produced dark mucous mass of hyaline arthrospores on mycelium. The coremia exclusively produced dikaryotic arthrospores with the remnant of a clamp connection. Continuous cell extension and division in the coremium stipe supplied cells for arthroconidiation at the coremium apex, which is surrounded by a liquid droplet (coremioliquid). The black coloured coremea (conidia) were produced by Antromycopsis macrocarpa (anamorph of P. cystidiosus) when cultured on potato dextrose agar medium. The agar plate was incubated at continuous light illumination for high amount of pigment (coremea) production. The slimy layer of the coremea was extracted and partially purified by alkaline and acid treatment. The black pigment was confirmed as melanin based on UV, IR and EPR spectra apart from chemical analysis. This is the first report on characterization of melanin obtained from Pleurotus cystidiosus var. formosensis.

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Section: Treatment With Melanin Synthesis Inhibitorsmentioning

confidence: 89%

Section: Spectroscopic Studiesmentioning

confidence: 99%

Section: Spectroscopic Studiesmentioning

confidence: 99%

Section: Treatment With Melanin Synthesis Inhibitorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation and characterization of melanin pigment from Pleurotus cystidiosus (telomorph of Antromycopsis macrocarpa)

Selvakumar

Rajasekar

Periasamy

et al. 2008

World J Microbiol Biotechnol

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Guaiacol as a natural melanin biosynthesis inhibitor to control northern corn leaf blight

Wang

Yuan

et al. 2022

Pest Management Science

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BACKGROUND The natural 1,8‐dihydroxynaphthalene (DHN) melanin biosynthesis inhibitors (MBIs) are one of the promising approaches to the integrated management of plant diseases but have received scarce attention until now. Herein, to explore the natural DHN MBIs used in the control of northern corn leaf blight (NCLB), a library of 53 essential oil compounds was used to screen the MBIs against Exserohilum turcicum, the causal pathogen of NCLB, using tricyclazole as a reference compound. RESULTS The results of morphological change in the colony, thermogravimetric analysis, ultraviolet–visible spectroscopy, and transmission electron microscopy confirmed that guaiacol could effectively inhibit the melanin production at 50 μg/mL under in vitro conditions. The in vitro bioassay results indicated that this inhibition effect was concentration‐dependent and the minimum inhibition concentration of guaiacol was 50 μg/mL. The in vivo experimental results demonstrated that guaiacol significantly inhibited appressorium formation and penetration on corn leaf sheaths at the concentration of 500 μg/mL. The pot experiment results revealed that there were no differences between guaiacol (500 μg/mL) and tricyclazole (100 μg/mL) in control efficacy. The enzymatic assay suggested that guaiacol might exert the activity through inhibiting DHN polymerization to form melanins, which was distinct from tricyclazole. CONCLUSIONS Taken together, this study screened out guaiacol as a natural MBI from 53 essential oil compounds and verified its effectiveness in the control of NCLB at 500 μg/mL. Above all, this research opened an avenue for exploring natural DHN MBIs in the integrated management of plant diseases. © 2022 Society of Chemical Industry.

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Relation of Halotolerance to Human-Pathogenicity in the Fungal Tree of Life: An Overview of Ecology and Evolution under Stress

Hoog

Zalar

Ende

et al. 2005

Cellular Origin, Life in Extreme Habitats and Astrobiology

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Isolation and Characterization of Melanin from a Marine Fungus

Cited by 43 publications

References 14 publications

Isolation and characterization of melanin pigment from Pleurotus cystidiosus (telomorph of Antromycopsis macrocarpa)

Isolation and characterization of melanin pigment from Pleurotus cystidiosus (telomorph of Antromycopsis macrocarpa)

Guaiacol as a natural melanin biosynthesis inhibitor to control northern corn leaf blight

Relation of Halotolerance to Human-Pathogenicity in the Fungal Tree of Life: An Overview of Ecology and Evolution under Stress

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