Isolation and Characterization of Lysinibacillus Sphaericus as Potential Bacteria for Toxic Metals Bioremediation in Industrial Wastewater of Western Himalayan Region

Abstract

The discharge of industrial waste effluent contains toxic substances which cause serious threat to human health as well as environment. The present study was conducted to determine the isolation and characterization of heavy metal removing Bacteria and its efficacy for removing Pb, Cd, Cu and Ni toxicity. In this study, wastewater samples were collected from different contaminated industrial sites in western Himalayan region. The isolate was recovered on nutrient agar plate supplemented with different concentration of Pb, Cd, Cu and Ni by the standard pour plate method. The isolated strain was characterized on the basis of morphological and biochemical test. The strain was identified as Lysinibacillus sphaericus. After identification heavy metal removal efficacy of the bacterial isolate for lead was 100% (1or 3ppm), 95% (5ppm), 90% (7ppm) and 85% (10 ppm). Heavy metal removal efficacy of the bacterial isolates for Cd was 100% (1ppm, 3ppm), 95% (5 and 7ppm) and 90% (10ppm). Heavy metal removal efficacy of the isolated bacteria for Ni was 100% (1ppm), 96% (3ppm), for 5ppm it removes 92%, 89% at 7ppm and at 10ppm the removal efficacy is 85%. Heavy metal removal efficacy of the isolated bacteria for Cu was 100% at 1ppm concentration, 98% at 3ppm, 94% at 5ppm, 91% at 7ppm and 88% at 10 ppm. The nucleotide sequences of Lysinibacillus sphaericus generated through 16S rDNA technique, the accession number provided to the bacterial sequence is OM904067. This strain was best for heavy metal tolerance and for bioremediation of heavy metal polluted environment.