Isolation and characterization of lung resident mesenchymal stem cells capable of differentiating into alveolar epithelial type II cells

Abstract

Controversies and risks continue to be reported about exogenous mesenchymal stem cell-based therapies. In contrast with employing exogenous stem cells, making use of lung resident mesenchymal stem cells (LR-MSCs) could be advantageous. Our study sought to isolate the LR-MSCs and explore their potential to differentiate into alveolar epithelial type II cells (ATII cells). Total lung cells were first precultured, from which the Sca-1(+) CD45(-) CD31(-) population was purified using fluorescence activated cell sorting (FACS). By these methods, it would seem that the Sca-1(+) CD45(-) CD31(-) cells were LR-MSCs. Similar to bone marrow derived mesenchymal stem cells (BM-MSCs), these cells express Sca-1, CD29, CD90, CD44 and CD106, but not CD31 or CD45. They share the same gene expression file with the BM-MSCs and have a similar DNA content during long-term culturing. Furthermore, they could be serially passaged with all these properties being sustained. Above all, LR-MSCs could differentiate into ATII cells when co-cultured with ATII cells in a trans-well system. These findings demonstrated that the Sca-1(+) CD45(-) CD31(-) cells appear to be LR-MSCs that can differentiate into ATII cells. This approach may hold promise for their use in the treatment of lung disease.

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

“…Further, RT-PCR was also used to confirm expression of the specific genes like Sox9, collagen type II, Runx2 and Ppar-γ for chondrogenic, osteogenic and adipogenic, respectively. Similar findings have also been reported by other researchers (Fortier et al, 2011;Gade et al, 2013;Tan et al, 2013;Gao et al, 2014;Gong et al, 2014). In a report that compared tri-lineage differentiation potential of rMSCs with that of human showed higher osteogenic and adipogenic differentiation potential in later with no difference in relation to chondrogenic differentiation potential (Tan et al, 2013).…”

Nonadherent culture method downregulates stem cell antigen-1 expression in mouse bone marrow mesenchymal stem cells

“…Sca-1 is widely recognized as a marker that can be used to enrich stem cells in a number of tissues (18)(19)(20). In addition, Sca-1 expression has been shown to be enriched on isolated mouse BM MSCs with a regenerative and self-renewal capacity (15,19,21,22).…”

Stem Cells and Neuronal Differentiation

“…There are an increasing number of reports describing their presence in adipose tissue [137][138][139]; periodontal tissues such as dental pulp, dental ligament, follicle, and papilla [137,140]; peripheral blood [141]; umbilical cord Wharton's jelly, cord blood, and chorionic villi of the placenta [142][143][144][145]; amniotic fluid [146]; fetal liver [147]; and lung [148]. The stromal cell population isolated from these tissues of origin need to be characterized by the set of MSC criteria such as plastic adherence; expression of CD105, CD73, and CD90; negative expression of CD45, CD34, CD14 or CD11b, CD79 alpha or CD19, and HLA-DR surface molecules; and in vitro differentiation to osteoblasts, adipocytes, and chondroblasts [149].…”