Isolation and characterization of lumpy skin disease virus from cattle in India

Kumar, Naveen; Chander, Yogesh; Kumar, Ram; Khandelwal, Nitin; Riyesh, Thachamvally; Chaudhary, Khushboo; Karuppusamy, S.; Kumar, Sanjit; Kumar, Anand; Gupta, M. K.; Pal, Yash; Barua, Sanjay; Tripathi, Bhupendra Nath

doi:10.1371/journal.pone.0241022

Cited by 78 publications

(72 citation statements)

References 27 publications

(49 reference statements)

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“…The pathology investigation in present study revealed that the animals exhibited characteristic lesions of LSD such as fever, cutaneous nodules, lymphadenopathy, pustules on various body parts, lameness, etc., which were described earlier also (Prozesky, & Barnard1982;Abdallah, et al, 2018;Sanz-Bernardo et al, 2020;Sudhakar et al, 2020;Kumar et al, 2021). This study observed morbidity rate of 4.48%, which is in the range of the LSD morbidity rate reported earlier (Sudhakar et al, 2020;Kumar et al, 2021).…”

Section: Discussionsupporting

confidence: 83%

“…The serological assays are not useful to distinguish different strains of Capri pox viruses (CaPVs), therefore, molecular studies are useful to distinguish LSDV, SPPV and GTPV (Tulman et al, 2002). Sequencing the host-specific G-protein-coupled chemokine receptor (GPCR), or RNA polymerase (RPO30) genes, species-specific molecular assays have been developed and validated for differentiation of CaPVs and also for phylogenetic grouping of field strains of LSDV (Lamien et al, 2011;Kumar et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Many states in India have reported LSDV occurrence which included Maharashtra, Gujarat, MP, Goa, Karnataka, Kerala, Andhra Pradesh, Odisha, West Bengal and North-Eastern states. Based on phylogenetic analysis, the strains circulating in India were reported to be genetically close to South African NI2490/KSGP-like strains (Sudhakar et al 2020) and Kenyan LSDV strains (Kumar et al, 2021). Various workers demonstrated gel-based PCR and real-time PCR assays for the detection of LSDV in bovines and differentiation between wild-type LSDV and vaccine virus strains (Lamien, et al, 2011;Vidanovic et al, 2016;Pestova et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Lumpy skin disease (LSD): Pathomorphological features and molecular detection in dairy cattle of west coastal India

Nayakvadi

Joshi

Rajkumar

et al. 2021

Preprint

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Lumpy skin disease (LSD) is an emerging pox viral disease affecting cattle population worldwide. In India, the first outbreak of LSD is reported during August 2019 in Odisha state, which then followed by outbreaks in crossbred and indigenous cattle population of other states. Present investigation designed to study the prevalence, pathomorphological changes and molecular detection of LSD virus in naturally infected cattle. The overall morbidity of LSD was 4.48% among 30 dairy farms. Skin nodular biopsy, whole blood and serum samples (n= 66) were collected for the diagnosis of LSD by histopathology, PCR and sequencing. The envelope protein gene (P32), Fusion protein (F) and DNA dependent RNA polymerase 30 kDa subunit (RPO30) genes were targeted for PCR testing. Out of 66, 46 cattle showed generalized skin nodules and papules of various sizes (0.5 - 6.5cm) on the skin particularly at neck, face, nose, tail, perineum and udder. Microscopic examination of the skin nodule biopsy tissue revealed presence of diffuse granulomatous inflammation, hyperkeratosis, focal to diffuse vasculitis and lymphangitis, vacuolar degeneration, spongiosis and acanthosis. The inflammatory cells typically comprised of macrophages, lymphocytes, neutrophils and eosinophils along with diffuse necrosis in dermis in chronic cases. The eosinophilic intracytoplasmic viral inclusions in keratinocytes and epithelial cells were detected in few cases. Gel-PCR assay detected P32 gene in 83%, F gene in 72% and RPO30 gene in 77% of skin biopsy samples. Three blood samples were also found positive for P32 gene by PCR. Whereas TaqMan™ probe Real Time PCR targeting EEV glycoprotein gene (LSDV126) detected LSDV in 94% of biopsy samples and three blood samples which indicated its higher sensitive for the diagnosis of LSDV. Phylogenetic analysis of RPO30 gene sequence showed that the isolates from this study were grouped in same cluster with LSDV isolates of Bangladesh, Kenya and other Indian isolates detected during 2019-20.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lumpy skin disease (LSD): Pathomorphological features and molecular detection in dairy cattle of west coastal India

Nayakvadi

Joshi

Rajkumar

et al. 2021

Preprint

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“…We noticed that the results of growth kinetics come similar to that obtained by growth curve as the best harvestation time was 4-5 DPI. This previous kinetics and Growth curve come in agree with Kumar et al (2021) whose kinetics of the viral DNA synthesis and one-step growth curve analysis suggested that Vero cell-adapted LSDV initiates synthesizing its genome at ~24 post-infection (hpi) with a peak level at ~96 hpi whereas evidence of progeny virus particles was observed at 36-48 h with a peak titer at ~120 h. To detect whether the virus antigen existed, we performed an IFA test on CPE-positive Vero cells. Virus-specific green fluorescence was found in the cytoplasm of infected cells (Fig.…”

Section: Discussionsupporting

confidence: 82%

Adaptation and Comparative Growth Behaviour of Egyptian SPV isolates in Vero

Elbagory

Soud

Hassan

2021

Benha Veterinary Medical Journal

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Although primary lamb testis (LT) cell culture seemed to be sensitive and the most suitable for adaptation of recently isolated sheep poxvirus (SPV), giving rapid and complete Cytopathic Effect (CPE), these cells were difficult to be maintained as a monolayer cell line. Moreover, their primary and secondary cultures still have many of the detrimental, inherent characteristics and contaminating elements. Therefore, the use of more stable cell lines (Vero cells) becomes a necessity for an economic preparation of SPV seed vaccine. Three SPV isolates, which previously passaged and adapted in lamb testis cell culture (isolates with titer reaching to 6.5 Log10 TCID50 / ml), were chosen to be transferred and propagated on Vero cell line. With further studies on its growth curve and growth kinetics to identify the growth behavior of these isolates, followed by detection of the virus antigen in the infected Vero cells by indirect florescent antibody technique (IFAT) as the first step for antigenic identification of the isolated adapted virus. The chosen optimum multiplicity of infection (MOI) was (0.01) with the optimum virus harvestation time was 4 days post-inoculation (DPI) and virus titre was equal to 10 5.5 . Finally, we recommend the use of Vero cells as a continuous cell line for preparation of seed of SPV prepared from an Egyptian isolate.

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“…Pada kulit yang mengalami nekrotik, virus pada nodul dapat bertahan hingga 33 hari atau lebih, pada kerak kering hingga 35 hari, dan setidaknya 18 hari dalam kulit yang dikeringkan. Namun virus peka terhadap sinar matahari dan deterjen (Kumar et al 2021;OIE 2017).…”

Section: Etiologi Dan Sifat Virus Lsd Etiologiunclassified

Lumpy Skin Disease: Ancaman Penyakit Emerging Bagi Kesehatan Ternak Sapi Di Indonesia

Sendow¹,

Assadah²,

Ratnawati³

et al. 2021

WARTAZOA

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Lumpy skin disease (LSD) menyebabkan kerugian ekonomi bagi peternak sapi, yang disebabkan oleh virus LSD, genus Capripoxvirus, famili Poxviridae. Penyakit ini di karakterisasi dengan adanya nodul pada kulit. Virus ini hanya menginfeksi sapi dan kerbau rawa yang memiliki tingkat mortalitas rendah, namun tingkat morbiditas tinggi. Sedangkan virus ini tidak menginfeksi kambing dan domba. Hingga saat ini, LSD belum pernah dilaporkan di Indonesia, oleh karena itu pengenalan penyakit LSD diperlukan terutama bagi dokter hewan dan paramedis lapangan sehingga infeksi LSD dapat diketahui dan penanganannya dapat dilakukan lebih dini. Tulisan ini akan membahas mengenai penyakit LSD, cara penyebaran penyakit, epidemiologi, diagnosis, faktor risiko serta pengendalian penyakit LSD, sehingga diharapkan masuknya infeksi LSD dapat terdeteksi sedini mungkin dan diantisipasi dengan lebih arif oleh pemangku kebijakan.

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Isolation and characterization of lumpy skin disease virus from cattle in India

Cited by 78 publications

References 27 publications

Lumpy skin disease (LSD): Pathomorphological features and molecular detection in dairy cattle of west coastal India

Lumpy skin disease (LSD): Pathomorphological features and molecular detection in dairy cattle of west coastal India

Adaptation and Comparative Growth Behaviour of Egyptian SPV isolates in Vero

Lumpy Skin Disease: Ancaman Penyakit Emerging Bagi Kesehatan Ternak Sapi Di Indonesia

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