Isolation and characterization of living circulating tumor cells in patients by immunomagnetic negative enrichment coupled with flow cytometry

Lu, Yusheng; Liang, Huaping; Yu, Ting; Xie, Jingjing; Chen, Shuming; Dong, Haiyan; Sinko, Patrick J.; Lian, Shu; Xu, Jianguo; Wang, Jichuang; Yu, Shasha; Shao, Jingwei; Yuan, Bo; Wang, Lie; Lee, Jia

doi:10.1002/cncr.29444

Cited by 62 publications

(58 citation statements)

References 47 publications

(52 reference statements)

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“…In our searching for novel therapies targeting CTC-based metastasis, we first characterized colorectal patients’ CTCs as Ep-CAM + CD45 − pancytokeratin + cell population 2 . We then revealed that these CTCs usually possess more than one type of surface biomarkers.…”

Section: Introductionmentioning

confidence: 99%

The traditional Chinese medicine Achyranthes bidentata and our de novo conception of its metastatic chemoprevention: from phytochemistry to pharmacology

Zhou

Qian

Dong

et al. 2017

Sci Rep

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Our recent biosystems analysis revealed similarities between embryonic implantation and cancer cell adhesion, which suggests that abortifacients may be good for safe and effective metastatic chemoprevention targeting circulating tumor cells (CTC). Here we test the hypothesis by using the well-known abortion herb Achyranthes bidentata Blume (A. bidentata). Five compounds were separated from the herb root. Among them, ginsenoside Ro was the most potent in inhibiting embryonic implantation within non-cytotoxic concentrations. It specifically inhibited the metastatic dissemination capability of colon cancer cells HT29, including the migration and invasion ability, and their adhesion to human endothelium through inhibiting integrin αvβ6, MMP-2, MMP-9, and ERK phosphorylation by HT29. Pretreatment of nude mice with oral ginsenoside Ro followed by HT29 intravenous inoculation and 40-day oral ginsenoside Ro significantly prevented lung metastasis with downregulation of integrin αvβ6 and no toxicity. The present study firstly introduces the new conception of utilizing safe and effective abortion botanic medicines for CTC-based metastatic chemoprevention.

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Section: Introductionmentioning

confidence: 99%

The traditional Chinese medicine Achyranthes bidentata and our de novo conception of its metastatic chemoprevention: from phytochemistry to pharmacology

Zhou

Qian

Dong

et al. 2017

Sci Rep

Self Cite

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“…These techniques generally rely on cell surface antigen expression for capturing CTCs or cell size to enrichment of CTCs by filtration techniques. CTC isolation methods and instruments were elaborately reported recently (8)(9)(10)(11). In our study, we used commercially available immunomagnetic beads coated with the monoclonal antibody Ber-EP4 (12), which recognizes specific epitopes of the extracellular domain of the EpCAM molecule.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of circulating tumor cells in advanced gastric cancer patients receiving chemotherapy

Liu¹,

Ling²,

Qi³

et al. 2017

Molecular and Clinical Oncology

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Abstract. The identification of circulating tumor cells (CTCs) may provide important prognostic information in several types of solid tumors, including gastric cancer. The aim of this study was to investigate whether CTC count may be used to predict survival in patients with advanced gastric cancer treated with chemotherapy. The CELLection™ Epithelial Enrich kit was used to isolate and purify CTCs from samples of peripheral blood. Immunofluorescent staining was used for CTC counting. High CTC counts were associated with poor tumor differentiation and high serum CEA levels (P=0.021 and 0.005, respectively). After 3 months, 16 patients with decreasing CTC counts after the first cycle of chemotherapy obtained complete response, partial response or stable disease, while 13 patients with increasing CTC counts developed progressive disease. The patients with decreasing CTC counts also exhibited longer progression-free survival (PFS) (P≤0.001) and overall survival (OS) (P= 0.002) compared with those with increasing CTC counts. Among all 59 patients, those with a CTC count of ≤2 cells̸5 ml blood exhibited longer PFS (P≤0.001) and OS (P≤0.001) compared with those with a CTC count of >2 cells̸5 ml blood. The multivariate analysis suggested that an increase of the CTC count after the first cycle of chemotherapy was only an independent prognostic marker of poor PFS (P=0.019). However, a baseline CTC count of >2 cells̸5 ml blood was an independent poor prognostic marker for PFS (P= 0.008) and OS (P= 0.001) in all 59 patients. Our study suggested that patients with a low baseline CTC count or decrease of the CTC count after the first cycle of chemotherapy may benefit significantly from palliative chemotherapy. In conclusion, CTC count may be a good chemotherapy monitoring marker and an ideal prognostic marker for patients receiving palliative chemotherapy.

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“…The assay was carried out as per the procedures described previously 34 . All samples were analyzed on a BD FACSAriaIII cell sorter (BD Bioscience, USA).…”

Section: Methodsmentioning

confidence: 99%

Downregulation of KCTD12 contributes to melanoma stemness by modulating CD271

Shen

et al. 2019

Cancer Biol Med

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Objective: Cancer metastasis remains the primary cause of cancer-related death worldwide. In a previous study, we found that levels of BTB/POZ domain-containing protein KCTD12 are lower in metastatic melanoma cells than in parental melanoma cells. The purpose of this study was to identify the roles of KCTD12 in cancer metastasis. Methods: The Cancer Genome Atlas (TCGA) datasets were used to evaluate the relationship between KCTD12 and skin cutaneous melanoma (SKCM) prognosis. The effects of endogenous KCTD12 on biological behaviors were examined using the MTT assay. The impacts of KCTD12 on melanoma stemness were explored using spheroid formation assay. KCTD12 knockout A375 cells were generated to confirm the inhibitory effect of KCTD12 on CD271, and a mouse metastatic model was used to determine the impact of KCTD12 on melanoma metastasis in vivo. Results: KCTD12 levels were lower in lung metastatic cells than in paired parental melanoma cells, and low KCTD12 expression indicated a poor prognosis in SKCM. Cancer metastasis-related capacities were higher in lung metastatic cells than in parental melanoma cells. Moreover, KCTD12 knockdown enhanced tumor growth and metastasis both in vitro and in vivo. Mechanistically, the interaction between KCTD12 and CD271 might be responsible for the stemness transformation after KCTD12 knockdown. Conclusions: This study identifies for the first time the role of the interaction between KCTD12 and CD271 in inducing melanoma cell stemness transformation. Moreover, KCTD12 repression enhances melanoma cell growth, adhesion, migration and invasion.

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Isolation and characterization of living circulating tumor cells in patients by immunomagnetic negative enrichment coupled with flow cytometry

Cited by 62 publications

References 47 publications

The traditional Chinese medicine Achyranthes bidentata and our de novo conception of its metastatic chemoprevention: from phytochemistry to pharmacology

The traditional Chinese medicine Achyranthes bidentata and our de novo conception of its metastatic chemoprevention: from phytochemistry to pharmacology

Prognostic value of circulating tumor cells in advanced gastric cancer patients receiving chemotherapy

Downregulation of KCTD12 contributes to melanoma stemness by modulating CD271

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